Application
Western blot
Sample
Human Cell lysate - whole cell (A549)
Loading amount
15 µg
Specification
A549
Treatment
50 uM BSO
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/2000
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Milk
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Donkey
Clonality: Polyclonal
Conjugation: HRP polymer
Dilution
1/5000
Detection
Detection method
HRP
Exposure
4 minute(s) and 0 second(s)
Bands
Specific: ~70 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Amelia Romoser
Verified customer
投稿 Jan 28 2011