製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Nrf2
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: IP, ChIP, IHC-Fr, WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Cow
  • 免疫原

    Recombinant fragment, corresponding to a region within internal sequence amino acids 108 to 413 of Human Nrf2 (Q16236).

  • ポジティブ・コントロール
    • HeLa cells; HeLa cell lysate; HeLa cytoplasmic lysate; HeLa membrane; HeLa nucleur lysate; HeLa cytoskeleton lysate; NIH-3T3 cell lysate; Human Cal27 xenograft tissue.
  • 特記事項
    NRF2 is bound to ubiquitin when it is the nucleus so MW would be higher than cytoplasm.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab137550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP 1/100 - 1/500.
ChIP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 24846271
WB 1/500 - 1/3000.

Detects a band of approximately 110 kDa (predicted molecular weight: 68 kDa).

ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.
  • 組織特異性
    Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.
  • 配列類似性
    Belongs to the bZIP family. CNC subfamily.
    Contains 1 bZIP domain.
  • ドメイン
    Acidic activation domain in the N-terminus, and DNA binding domain in the C-terminus.
  • 翻訳後修飾
    Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.
  • 細胞内局在
    Cytoplasm > cytosol. Nucleus. Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents.
  • Information by UniProt
  • 参照データベース
  • 別名
    • erythroid derived 2 antibody
    • HEBP1 antibody
    • like 2 antibody
    • NF E2 related factor 2 antibody
    • NF-E2-related factor 2 antibody
    • NF2L2_HUMAN antibody
    • NFE2 related factor 2 antibody
    • NFE2-related factor 2 antibody
    • Nfe2l2 antibody
    • Nrf 2 antibody
    • NRF2 antibody
    • Nuclear factor (erythroid derived 2) like 2 antibody
    • Nuclear factor antibody
    • nuclear factor erythroid 2 like 2 antibody
    • Nuclear factor erythroid 2 related factor 2 antibody
    • Nuclear factor erythroid 2-related factor 2 antibody
    • Nuclear factor erythroid derived 2 like 2 antibody
    see all

画像

  • All lanes : Anti-Nrf2 antibody (ab137550) at 1/1000 dilution

    Lane 1 : Non-transfected (-) 293T whole cell extracts
    Lane 2 : NRF2-transfected (+) 293T whole cell extracts

    Lysates/proteins at 30 µg per lane.
  • ab137550 staining Nrf2 in Rat liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/250 in BSA in TBS) for 30 minutes. A undiluted HRP-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.

    See Abreview

  • ab137550 staining Nrf2 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde. Samples were incubated with primary antibody diluted at 1/500 (green). Phalloidin, a cytoskeleton marker, diluted at 1/200 (red) was used.

  • ChIP was performed with HepG2 chromatin extract and 5 μg of either normal rabbit IgG or Anti-Nrf2 antibody (ab137550). The precipitated DNA was detected by PCR with primer set targeting to GCLC gene locus.

  • Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 cells labeling Nrf2 with ab137550 at 1/500 dilution (green). Cells were fixed in 4% paraformaldehyde at RT for 15 min. Hoechst nuclear staining (blue).

  • Anti-Nrf2 antibody (ab137550) at 1/500 dilution + C8D30 whole cell extract at 30 µg
  • ab137550 at 5 μg immunoprecipitating Nrf2 in HepG2 whole cell extracts.

    Lane 1 (-): Ctrl IgG instead of ab137550 in HepG2 whole cell extracts.
    Lane 2 (+): ab137550 + HepG2 whole cell extracts.

    For western blotting an anti-Rabbit IgG was used as a secondary reagent.

  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, labelling Nrf2 with an137550 at 1/200 dilution.
  • Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, labelling Nrf2 with ab137550 at 1/500 dilution.
  • All lanes : Anti-Nrf2 antibody (ab137550) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HeLa cytoplasmic lysate
    Lane 3 : HeLa nuclear lysate

    Lysates/proteins at 20 µg per lane.

参考文献

This product has been referenced in:
  • Ren HL  et al. Downregulated Nuclear Factor E2-Related Factor 2 (Nrf2) Aggravates Cognitive Impairments via Neuroinflammation and Synaptic Plasticity in the Senescence-Accelerated Mouse Prone 8 (SAMP8) Mouse: A Model of Accelerated Senescence. Med Sci Monit 24:1132-1144 (2018). Read more (PubMed: 29474348) »
  • Zhang L  et al. Mercaptoethanol Protects the Aorta from Dissection by Inhibiting Oxidative Stress, Inflammation, and Extracellular Matrix Degeneration in a Mouse Model. Med Sci Monit 24:1802-1812 (2018). Read more (PubMed: 29589594) »
See all 39 Publications for this product

レビューと Q&A

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1-3 of 3 Abreviews

Application
Western blot
Sample
Bos taurus Cell lysate - whole cell (MAC-T cell line)
Gel Running Conditions
Non-reduced Denaturing (10%)
Loading amount
60 µg
Treatment
transfected with shnrf2
Specification
MAC-T cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

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Verified customer

投稿 Jun 01 2017

Application
Western blot
Loading amount
25000 cells
Gel Running Conditions
Reduced Denaturing (10)
Sample
Human Cell lysate - whole cell (Prostate)
Specification
Prostate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Verified customer

投稿 Jul 29 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Liver)
Specification
Liver
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
Permeabilization
No

Abcam user community

Verified customer

投稿 Jan 29 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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