Anti-NrCAM 抗体 - Neuronal Marker (ab24344)

Thank you for your enquiry. For further information about the protein sequence, please refer to the publication below. http://www.jcb.org/cgi/reprint/113/6/1399 Please bear in mind that this product has not been tested in flow cytometry and therefore, we cannot guarantee that it will work well in your research. Unfortunately, we do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. If the antibody is being used in an untested species or application, we cannot offer a replacement or refund; however, we invite you to use our Abreview system. The Abreview system allows researchers to submit reviews of our products in exchange for Abpoints. Abpoints can be redeemed for discounts on future antibody purchases or rewards such as Amazon.com gift certificates. Should you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview right from the online datasheet. To find out more about our Abreview system, please see the following URL: https://www.abcam.com/abreviews I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Yes, Karen and I spoke and decided on the advice Karen gave to you. I have still have the same comments. What results did you obtain from the no primary control you ran? If you are still seeing the high background with the secondary only, have you tried a different secondary? The SuperSignal detection system is extremely sensitive, and it may be necessary to dilute out the primary antibody further than you have. Have you tried using ECL+ or ECL instead of SuperSignal? In addition, we would let our blots stand before exposing to film since the signal was so strong. I would suggest either trying a less sensitive ECL system, using less primary antibody (try 1/5000), or letting the blot stand for a while before exposing them to the film. I am not surprised that other antibodies have worked well in this system because super signal is a good system. I am concerned that the antibody was optimized at 1/500 dilution + 20 µg of postnatal day 8 mouse brain extract in SDS using ECL plus and under the conditions you have tried with the supersignal system you have a completely black blot suggesting overexposure, too much antibody, too much supersignal etc. I really believe you need to optimize the antibody based on our suggestions.

Thank you for your reply and attaching the images. You have a very poor result but at this point I have no way of determining if the antibody is causing the problem or not. It would be highly unusual that an antibody would yield a pattern of staining an entire membrane whether there is protein present or not. I will not be able to issue a refund unless you fill out the form at the following link completely, you have purchased the antibody in the last 90 days, and we determine that we do not have any additional suggestions to give you. Please fill out the form at the following link so that I may be able to better assist you: https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=24344&mode=questionaire I look forward to hearing from you,

I'm sorry to hear you are having a problem with ab24344. I would like to suggest the following modifications to your protocol: Please use a fresh blot and try: blocking in 5% milk for 30 min, incubating in primary overnight in 5% milk at 4 degrees, and then incubate in secondary for 1 hour at room temperature. Please also fill out the form at the following link: https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=24344&mode=questionaire so I have more information and send me a picture of your blot. I look forward to your reply.