Anti-NRAS 抗体 (ab77392)
Key features and details
- Goat polyclonal to NRAS
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-NRAS antibody
NRAS 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to NRAS -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide within Human NRAS aa 150 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Accession Number: NP_002515.1
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ポジティブ・コントロール
- WB: MCF7 cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab77392 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab77392の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (6) |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
1 hour primary incubation at room temperature is recommended for this product. |
特記事項 |
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WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). 1 hour primary incubation at room temperature is recommended for this product. |
ターゲット情報
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機能
Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. -
関連疾患
Defects in NRAS are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia.
Defects in NRAS are the cause of Noonan syndrome type 6 (NS6) [MIM:613224]. A syndrome characterized by facial dysmorphic features such as hypertelorism, a downward eyeslant and low-set posteriorly rotated ears. Other features can include short stature, a short neck with webbing or redundancy of skin, cardiac anomalies, deafness, motor delay and variable intellectual deficits. -
配列類似性
Belongs to the small GTPase superfamily. Ras family. -
翻訳後修飾
Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi. -
細胞内局在
Cell membrane. Golgi apparatus membrane. Shuttles between the plasma membrane and the Golgi apparatus. - Information by UniProt
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参照データベース
- Entrez Gene: 4893 Human
- Entrez Gene: 18176 Mouse
- Entrez Gene: 24605 Rat
- Omim: 164790 Human
- SwissProt: P01111 Human
- SwissProt: P08556 Mouse
- SwissProt: Q04970 Rat
- Unigene: 486502 Human
see all -
別名
- ALPS4 antibody
- AV095280 antibody
- GTPase NRas antibody
see all
画像
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (12)
ab77392 は 12 報の論文で使用されています。
- Yan Y et al. NRAS expression is associated with prognosis and tumor immune microenvironment in lung adenocarcinoma. J Cancer Res Clin Oncol 148:565-575 (2022). PubMed: 34746975
- Ma Y et al. Neuronal miR-29a protects from obesity in adult mice. Mol Metab 61:101507 (2022). PubMed: 35490865
- Nam HJ et al. GPR110 promotes progression and metastasis of triple-negative breast cancer. Cell Death Discov 8:271 (2022). PubMed: 35614051
- Bery N et al. A potent KRAS macromolecule degrader specifically targeting tumours with mutant KRAS. Nat Commun 11:3233 (2020). PubMed: 32591521
- Dey S et al. miR-29a Is Repressed by MYC in Pancreatic Cancer and Its Restoration Drives Tumor-Suppressive Effects via Downregulation of LOXL2. Mol Cancer Res 18:311-323 (2020). PubMed: 31662451