保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
バッファーPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
精製度Protein A purified
Our Abpromise guarantee covers the use of ab62601 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2.5 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 61 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/12500.
機能Binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development.
組織特異性Abundantly expressed in pancreas, less in liver, very low levels in heart and lung. Expressed in the Hep-G2 cell line. Isoform 1 and isoform 2 seem to be present in fetal and adult liver and Hep-G2 cells.
配列類似性Belongs to the nuclear hormone receptor family. NR5 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
- Information by UniProt
- Alpha-1-fetoprotein transcription factor antibody
- B1-binding factor antibody
- B1F antibody
Lane 1 : Marker
Lane 2 : Anti-NR5A2 / LRH1 antibody (ab62601) at 2.5 µg/ml
Lane 2 : HepG2 cell lysate at 10 µg
Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 61 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Gel concentration 12%
ab62601 has not yet been referenced specifically in any publications.