Anti-NR2F2 抗体 [H7147] (ab41859)
Key features and details
- Mouse monoclonal [H7147] to NR2F2
- Suitable for: IP, ELISA, IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-NR2F2 antibody [H7147]
NR2F2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [H7147] to NR2F2 -
由来種
Mouse -
アプリケーション
適用あり: IP, ELISA, IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Cow -
免疫原
Recombinant fragment:
PGQGGPAS TPAQTAAGGQ GGPG
, corresponding to amino acids 43-64 of Human NR2F2 -
ポジティブ・コントロール
- Human Convoluted tubule and Rat Glomerular
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特記事項
This product was changed from ascites to tissue culture supernatant on 3rd April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Physiological saline. -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
H7147 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab41859の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 49 kDa (predicted molecular weight: 46 kDa).
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ICC/IF | (1) |
Use at an assay dependent concentration.
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特記事項 |
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IP
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 49 kDa (predicted molecular weight: 46 kDa). |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
Ligand-activated transcription factor. Activated by high concentrations of 9-cis-retinoic acid and all-trans-retinoic acid, but not by dexamethasone, cortisol or progesterone (in vitro). Regulation of the apolipoprotein A-I gene transcription. Binds to DNA site A. -
組織特異性
Ubiquitous. -
関連疾患
Congenital heart defects, multiple types, 4 -
配列類似性
Belongs to the nuclear hormone receptor family. NR2 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 281945 Cow
- Entrez Gene: 7026 Human
- Entrez Gene: 11819 Mouse
- Entrez Gene: 113984 Rat
- Omim: 107773 Human
- SwissProt: Q9TTR7 Cow
- SwissProt: P24468 Human
- SwissProt: P43135 Mouse
see all -
別名
- Apolipoprotein A-I regulatory protein 1 antibody
- Apolipoprotein AI regulatory protein 1 antibody
- ARP-1 antibody
see all
画像
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ab41859 staining human NR2F2 in convoluted tubule by Immunohistochemistry (FFPE-sections) 10ug/ml.
This image was generated using the ascites version of the product.
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ab41859 staining rat NR2F2 in Glomerular by Immunohistochemistry (FFPE-sections) 10ug/ml.
This image was generated using the ascites version of the product.
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All lanes : Anti-NR2F2 antibody [H7147] (ab41859) at 1 µg/ml
Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Additional bands at: 72 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutesThis image was generated using the ascites version of the product.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (23)
ab41859 は 23 報の論文で使用されています。
- Polvani S et al. Isoforms of the orphan nuclear receptor COUP‑TFII differentially modulate pancreatic cancer progression. Int J Oncol 60:N/A (2022). PubMed: 35348189
- Meçe O et al. Lipid droplet degradation by autophagy connects mitochondria metabolism to Prox1-driven expression of lymphatic genes and lymphangiogenesis. Nat Commun 13:2760 (2022). PubMed: 35589749
- Wei JR et al. Identification of visual cortex cell types and species differences using single-cell RNA sequencing. Nat Commun 13:6902 (2022). PubMed: 36371428
- O'Leary TP et al. Neuronal cell types, projections, and spatial organization of the central amygdala. iScience 25:105497 (2022). PubMed: 36425768
- Aguiari P et al. Persistent COUP-TFII expression underlies the myopathy and impaired muscle regeneration observed in resistance to thyroid hormone-alpha. Sci Rep 11:4601 (2021). PubMed: 33633251