Anti-NR0B1 / Dax1 抗体 (ab97369)
Key features and details
- Rabbit polyclonal to NR0B1 / Dax1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-NR0B1 / Dax1 antibody
NR0B1 / Dax1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NR0B1 / Dax1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment: APEATLGPCW GCSCGSDPGV GRAGLGGRPV ALLYRCCFCG EDHPRQGSIL YSLLTSSKQT HVAPAAPEAR PGGAWWDRSY FAQRPGGKEA LPGGRATALL YRCCFCGEDH PQQGSTLYCV PTSTNQAQAA PEERPRAPWW DTSSGALRPV ALKSPQVVCE AASAGLLKTL RFVKYLPCFQ VLPLDQQLVL VRNCWASLLM LELAQDRLQF ETVEVSEPSM LQKILTTRRR ETGGNEPLPV PTLQHHLAPP AEARKVPSAS QVQAIKCFLS KCWSLNISTK , corresponding to amino acids 96 - 376 of Human NR0B1/ Dax1 (NP_000466).
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ポジティブ・コントロール
- WB: 293T, A431, H1299, HeLaS3, HepG2 and Raji cell lysates IF: HeLa cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97369の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 52 kDa.
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ICC/IF |
1/100 - 1/200.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 52 kDa. |
ICC/IF
1/100 - 1/200. |
ターゲット情報
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機能
Orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency. -
関連疾患
Defects in NR0B1 are the cause of X-linked adrenal hypoplasia congenital (XL-AHC) [MIM:300200]; also known as X-linked Addison disease (AHX). XL-AHC is a developmental disorder of the adrenal gland that results in profound hormonal deficiencies and is lethal if untreated. It is characterized by the absence of the permanent zone of the adrenal cortex and by a structural disorganization of the glands. Hypogonadotropic hypogonadism (HHG) is frequently associated with this disorder. HHG is a condition resulting from or characterized by abnormally decreased gonadal function, with retardation of growth and sexual development.
Defects in NR0B1 are the cause of 46,XY sex reversal type 2 (SRXY2) [MIM:300018]. It is a condition characterized by male-to-female sex reversal in the presence of a normal 46,XY karyotype. Note=XY individuals with a duplication of part of the short arm of the X chromosome and an intact SRY gene develop as females. The single X chromosome in these individuals does not undergo X-chromosome inactivation; therefore, these individuals presumably carry 2 active copies of genes, including the NR0B1 gene, in the duplicated region. Individuals with deletion of this region develop as males. Genes within the dosage-sensitive sex reversal region are, therefore, not essential for testis development, but, when present in a double dose, interfere with testis formation. -
配列類似性
Belongs to the nuclear hormone receptor family. NR0 subfamily. -
ドメイン
Homodimerization involved an interaction between amino and carboxy termini involving LXXLL motifs and steroid binding domain (AF-2 motif). Heterodimerizes with NR5A1 and NROB2 through its N-terminal LXXLL motifs. -
細胞内局在
Nucleus. Cytoplasm. Shuttles between the cytoplasm and nucleus. Homodimers exits in the cytoplasm and in the nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 190 Human
- Omim: 300473 Human
- SwissProt: P51843 Human
- Unigene: 268490 Human
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別名
- AHC antibody
- AHCH antibody
- AHX antibody
see all
画像
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab97369 は 1 報の論文で使用されています。
- Johnson KM et al. Role for the EWS domain of EWS/FLI in binding GGAA-microsatellites required for Ewing sarcoma anchorage independent growth. Proc Natl Acad Sci U S A 114:9870-9875 (2017). PubMed: 28847958