Anti-NQO1 抗体 (ab97385)
Key features and details
- Rabbit polyclonal to NQO1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-NQO1 antibody
NQO1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NQO1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide contain a sequence corresponding to a region within amino acids 213 and 224 of Human NQO1 9(NP_000894).
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ポジティブ･コントロール
- 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates for WB; HeLa cells for IF; CL1-0 xenograft for IHC-P.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97385の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 31 kDa. In some tested cell lines double bands (~26-34kDa) are seen: 293T, A431, H1299, HeLa, HepG2.
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| IHC-P | (1) |
1/100 - 1/500.
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| ICC/IF |
1/100 - 1/200.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 31 kDa. In some tested cell lines double bands (~26-34kDa) are seen: 293T, A431, H1299, HeLa, HepG2. |
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IHC-P
1/100 - 1/500. |
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ICC/IF
1/100 - 1/200. |
ターゲット情報
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機能
The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. -
配列類似性
Belongs to the NAD(P)H dehydrogenase (quinone) family. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 1728 Human
- Omim: 125860 Human
- SwissProt: P15559 Human
- Unigene: 406515 Human
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別名
- Azoreductase antibody
- Cytochrome b 5 reductase antibody
- DHQU antibody
see all
画像
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Western blot - Anti-NQO1 antibody (ab97385)All lanes : Anti-NQO1 antibody (ab97385) at 1/1000 dilution
Lane 1 : MOLT4 whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 31 kDa
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-NQO1 antibody (ab97385)Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab97385 at 1/200 dilution. Lower image merged with DNA probe. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NQO1 antibody (ab97385)Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using ab97385 at 1/500 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NQO1 antibody (ab97385)Image from Hevir N et al., Molecular and Cellular Endocrinology, Volume 367, Issue1-2, March 2013, Pages 74-84. Fig 4.; doi: 10.1016/j.mce.2012.12.019. Epub 2012 Dec 28 with permission from Elsevier.Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human ovarian endometriosis tissue, staining NQO1 with ab97385.
Antigen retrieval was performed in sodium citrate buffer in a microwave oven. Samples were blocked with rabbit serum for one hour before incubating overnight with primary antibody (1/200). Stainined was detected using DAB.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab97385 は 4 報の論文で使用されています。
- Zhi W et al. Melatonin elicits protective effects on OGD/R-insulted H9c2 cells by activating PGC-1a/Nrf2 signaling. Int J Mol Med 45:1294-1304 (2020). PubMed: 32323734
- Shi X & Fu L Piceatannol inhibits oxidative stress through modification of Nrf2-signaling pathway in testes and attenuates spermatogenesis and steroidogenesis in rats exposed to cadmium during adulthood. Drug Des Devel Ther 13:2811-2824 (2019). PubMed: 31496657
- Ravasz D et al. Reduction of 2-methoxy-1,4-naphtoquinone by mitochondrially-localized Nqo1 yielding NAD+ supports substrate-level phosphorylation during respiratory inhibition. Biochim Biophys Acta N/A:N/A (2018). WB . PubMed: 29746824
- Hevir N et al. Disturbed balance between phase I and II metabolizing enzymes in ovarian endometriosis: A source of excessive hydroxy-estrogens and ROS? Mol Cell Endocrinol : (2012). WB, IHC-P ; Human . PubMed: 23277161