製品の概要
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製品名Anti-NQO1 antibody
NQO1 一次抗体 製品一覧 -
製品の詳細Goat polyclonal to NQO1
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由来種Goat
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特異性This products is expected to recognize all three reported isoforms (NP_000894.1; NP_001020604.1; NP_001020605.1)
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アプリケーション適用あり: ICC/IF, WB, IHC-FoFr, ELISA, IHC-Frmore details
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種交差性交差種: Mouse, Rat, Dog, Human
交差が予測される動物種: Pig
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免疫原
Synthetic peptide: SIPTDNQIKARK, corresponding to C terminal amino acids 267-274 of Human NQO1.
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ポジティブ･コントロール
- Human kidney, lung and liver lysate
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特記事項Principal Names – NQO1; NAD(P)H dehydrogenase, quinone 1; DTD; QR1; DHQU; DIA4; NMOR1; NMORI; diaphorase-4; diaphorase (NADH/NADPH); diaphorase (NADH/NADPH) (cytochrome b-5 reductase); NAD(P)H:menadione oxidoreductase 1, dioxin-inducible 1. Official Gene Symbol - NQO1. GenBank Accession Number – NP_000894. LocusLink ID - 1728. Gene Ontology terms - cytoplasm; xenobiotic metabolism; detoxification response; cytochrome b5 reductase; nitric oxide biosynthesis; NAD(P)H dehydrogenase (quinone); synaptic transmission, cholinergic.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab2346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use a concentration of 1 µg/ml. | |
| WB | Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 26-30 kDa.Can be blocked with Human NQO1 peptide (ab22904). | |
| IHC-FoFr | 1/250. PubMed: 19186165 | |
| ELISA | 1/64000. | |
| IHC-Fr | Use at an assay dependent dilution. PubMed: 7715346 |
ターゲット情報
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機能The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
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配列類似性Belongs to the NAD(P)H dehydrogenase (quinone) family.
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細胞内局在Cytoplasm.
- Information by UniProt
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参照データベース
- Entrez Gene: 610935 Dog
- Entrez Gene: 1728 Human
- Entrez Gene: 18104 Mouse
- Entrez Gene: 100286873 Pig
- Entrez Gene: 24314 Rat
- Omim: 125860 Human
- SwissProt: P15559 Human
- SwissProt: Q64669 Mouse
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別名
- Azoreductase antibody
- Cytochrome b 5 reductase antibody
- DHQU antibody
see all
画像
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Western blot - Anti-NQO1 antibody (ab2346)This image was kindly supplied as part of the review submitted by Lauren Aleksunes. Various mouse liver cytosol samples and human recombinant protein were run on the gel and then incubated with ab2346. This image was kindly supplied as part of the review submitted by Lauren Aleksunes. Various mouse liver cytosol samples and human recombinant protein were run on the gel and then incubated with ab2346. -
Western blot - Anti-NQO1 antibody (ab2346)Anti-NQO1 antibody (ab2346) at 0.03 µg/ml + Human Kidney lysate at 35 µg
Observed band size: 30 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour. Detected by chemiluminescence. -
Immunocytochemistry/ Immunofluorescence - Anti-NQO1 antibody (ab2346)ICC/IF image of ab2346 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2346, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
参考文献
This product has been referenced in:
- Lv W et al. Schwann Cell Plasticity is Regulated by a Weakened Intrinsic Antioxidant Defense System in Acute Peripheral Nerve Injury. Neuroscience 382:1-13 (2018). Read more (PubMed: 29684504) »
- Forootan SS et al. Real-time in vivo imaging reveals localised Nrf2 stress responses associated with direct and metabolism-dependent drug toxicity. Sci Rep 7:16084 (2017). Read more (PubMed: 29167567) »
レビューと Q&A
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