Anti-NQO1 抗体 (ab2346)
Key features and details
- Goat polyclonal to NQO1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Rat, Human, Pig
- Isotype: IgG
製品の概要
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製品名
Anti-NQO1 antibody
NQO1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to NQO1 -
由来種
Goat -
特異性
This products is expected to recognize all three reported isoforms (NP_000894.1; NP_001020604.1; NP_001020605.1) -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Rat, Human, Pig
交差が予測される動物種: Dog
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免疫原
Synthetic peptide corresponding to Human NQO1 aa 267-274 (C terminal).
Sequence:C-SIPTDNQIKARK
(Peptide available asab22904) -
特記事項
Principal Names – NQO1; NAD(P)H dehydrogenase, quinone 1; DTD; QR1; DHQU; DIA4; NMOR1; NMORI; diaphorase-4; diaphorase (NADH/NADPH); diaphorase (NADH/NADPH) (cytochrome b-5 reductase); NAD(P)H:menadione oxidoreductase 1, dioxin-inducible 1. Official Gene Symbol - NQO1. GenBank Accession Number – NP_000894. LocusLink ID - 1728. Gene Ontology terms - cytoplasm; xenobiotic metabolism; detoxification response; cytochrome b5 reductase; nitric oxide biosynthesis; NAD(P)H dehydrogenase (quinone); synaptic transmission, cholinergic.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab2346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use a concentration of 5 - 10 µg/ml. | |
| WB | Use a concentration of 0.03 - 0.3 µg/ml. Detects a band of approximately 26-30 kDa. Primary incubation for 1 hour at room temperature. |
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| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. -
配列類似性
Belongs to the NAD(P)H dehydrogenase (quinone) family. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 610935 Dog
- Entrez Gene: 1728 Human
- Entrez Gene: 100286873 Pig
- Entrez Gene: 24314 Rat
- Omim: 125860 Human
- SwissProt: P15559 Human
- SwissProt: P05982 Rat
- Unigene: 406515 Human
see all -
別名
- Azoreductase antibody
- Cytochrome b 5 reductase antibody
- DHQU antibody
see all
画像
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Western blot - Anti-NQO1 antibody (ab2346)Anti-NQO1 antibody (ab2346) at 0.1 µg + U251 cell lysate in RIPA buffer at 35 µgDetected using chemiluminescence.
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Immunocytochemistry/ Immunofluorescence - Anti-NQO1 antibody (ab2346)Immunocytochemistry/Immunofluorescence analysis of HepG2 labelling NQO1 with ab2346 at 5 µg/ml. Cells were paraformaldehyde fixed and permeabilized with 0.15% triton. Primary antibody icubation was for 1 hour followed by incubation with an Alexa Fluor® 488-conjugated secondary antibody at 2 µg/ml. DAPI nuclear counterstain was used.
Negative control: Unimmunized goat IgG (5 µg/ml) followed by an Alexa Fluor® 488-conjugated secondary antibody (2 µg/ml).
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Western blot - Anti-NQO1 antibody (ab2346)All lanes : Anti-NQO1 antibody (ab2346) at 0.03 µg
Lane 1 : Human kidney tissue lysate in RIPA buffer
Lane 2 : Human lung tissue lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.Detected using chemiluminescence.
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Immunocytochemistry/ Immunofluorescence - Anti-NQO1 antibody (ab2346)Immunocytochemistry/Immunofluorescence analysis of U251 labelling NQO1 with ab2346 at 10 µg/ml. Cells were paraformaldehyde fixed and permeabilized with 0.15% triton. Primary antibody icubation was for 1 hour followed by incubation with an Alexa Fluor® 488-conjugated secondary antibody at 2 µg/ml. DAPI nuclear counterstain was used.
Negative control: Unimmunized goat IgG (10 µg/ml) followed by an Alexa Fluor® 488-conjugated secondary antibody (2 µg/ml).
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Western blot - Anti-NQO1 antibody (ab2346)All lanes : Anti-NQO1 antibody (ab2346) at 1 µg
Lane 1 : Rat kidney tissue lysate in RIPA buffer
Lane 2 : Pig kidney tissue lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.Detected using chemiluminescence.
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Immunocytochemistry/ Immunofluorescence - Anti-NQO1 antibody (ab2346)ICC/IF image of ab2346 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2346, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
参考文献 (69)
ab2346 は 69 報の論文で使用されています。
- Salama RM et al. LCZ696 (sacubitril/valsartan) protects against cyclophosphamide-induced testicular toxicity in rats: Role of neprilysin inhibition and lncRNA TUG1 in ameliorating apoptosis. Toxicology 437:152439 (2020). PubMed: 32197949
- Huang C et al. TLR3 Ligand PolyI:C Prevents Acute Pancreatitis Through the Interferon-ß/Interferon-a/ß Receptor Signaling Pathway in a Caerulein-Induced Pancreatitis Mouse Model. Front Immunol 10:980 (2019). PubMed: 31130960
- Guo H et al. Apigetrin treatment attenuates LPS-induced acute otitis media though suppressing inflammation and oxidative stress. Biomed Pharmacother 109:1978-1987 (2019). PubMed: 30551453
- Yagishita Y et al. Nrf2 represses the onset of type 1 diabetes in non-obese diabetic mice. J Endocrinol N/A:N/A (2019). PubMed: 30625116
- Xiao X et al. Biogenic nanoselenium particles activate Nrf2-ARE pathway by phosphorylating p38, ERK1/2, and AKT on IPEC-J2 cells. J Cell Physiol 234:11227-11234 (2019). PubMed: 30488492
