Anti-NQO1 抗体 (ab2346)
Key features and details
- Goat polyclonal to NQO1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
製品の概要
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製品名
Anti-NQO1 antibody
NQO1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to NQO1 -
由来種
Goat -
特異性
This products is expected to recognize all three reported isoforms (NP_000894.1; NP_001020604.1; NP_001020605.1) -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human, Pig
交差が予測される動物種: Dog
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免疫原
Synthetic peptide corresponding to Human NQO1 aa 267-274 (C terminal).
Sequence:C-SIPTDNQIKARK
(Peptide available asab22904) -
ポジティブ･コントロール
- WB: U251 cell lysate, Human kidney, human lung, rat kidney and pig kidney tissue lysates. IHC-P: Human kidney tissue. ICC/IF: U251, HeLa and HepG2 cells.
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特記事項
Principal Names – NQO1; NAD(P)H dehydrogenase, quinone 1; DTD; QR1; DHQU; DIA4; NMOR1; NMORI; diaphorase-4; diaphorase (NADH/NADPH); diaphorase (NADH/NADPH) (cytochrome b-5 reductase); NAD(P)H:menadione oxidoreductase 1, dioxin-inducible 1. Official Gene Symbol - NQO1. GenBank Accession Number – NP_000894. LocusLink ID - 1728. Gene Ontology terms - cytoplasm; xenobiotic metabolism; detoxification response; cytochrome b5 reductase; nitric oxide biosynthesis; NAD(P)H dehydrogenase (quinone); synaptic transmission, cholinergic.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab2346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use a concentration of 5 - 10 µg/ml. | |
| WB | Use a concentration of 0.03 - 0.3 µg/ml. Detects a band of approximately 26-30 kDa. Primary incubation for 1 hour at room temperature. |
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| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. -
配列類似性
Belongs to the NAD(P)H dehydrogenase (quinone) family. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 610935 Dog
- Entrez Gene: 1728 Human
- Entrez Gene: 18104 Mouse
- Entrez Gene: 100286873 Pig
- Entrez Gene: 24314 Rat
- Omim: 125860 Human
- SwissProt: P15559 Human
- SwissProt: Q64669 Mouse
see all -
別名
- Azoreductase antibody
- Cytochrome b 5 reductase antibody
- DHQU antibody
see all
画像
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Western blot - Anti-NQO1 antibody (ab2346)Anti-NQO1 antibody (ab2346) at 0.1 µg + U251 cell lysate in RIPA buffer at 35 µgDetected using chemiluminescence.
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Immunocytochemistry/ Immunofluorescence - Anti-NQO1 antibody (ab2346)Immunocytochemistry/Immunofluorescence analysis of HepG2 labelling NQO1 with ab2346 at 5 µg/ml. Cells were paraformaldehyde fixed and permeabilized with 0.15% triton. Primary antibody icubation was for 1 hour followed by incubation with an Alexa Fluor® 488-conjugated secondary antibody at 2 µg/ml. DAPI nuclear counterstain was used.
Negative control: Unimmunized goat IgG (5 µg/ml) followed by an Alexa Fluor® 488-conjugated secondary antibody (2 µg/ml).
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Western blot - Anti-NQO1 antibody (ab2346)All lanes : Anti-NQO1 antibody (ab2346) at 0.03 µg
Lane 1 : Human kidney tissue lysate in RIPA buffer
Lane 2 : Human lung tissue lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.Detected using chemiluminescence.
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Immunocytochemistry/ Immunofluorescence - Anti-NQO1 antibody (ab2346)Immunocytochemistry/Immunofluorescence analysis of U251 labelling NQO1 with ab2346 at 10 µg/ml. Cells were paraformaldehyde fixed and permeabilized with 0.15% triton. Primary antibody icubation was for 1 hour followed by incubation with an Alexa Fluor® 488-conjugated secondary antibody at 2 µg/ml. DAPI nuclear counterstain was used.
Negative control: Unimmunized goat IgG (10 µg/ml) followed by an Alexa Fluor® 488-conjugated secondary antibody (2 µg/ml).
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Western blot - Anti-NQO1 antibody (ab2346)All lanes : Anti-NQO1 antibody (ab2346) at 1 µg
Lane 1 : Rat kidney tissue lysate in RIPA buffer
Lane 2 : Pig kidney tissue lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.Detected using chemiluminescence.
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Immunocytochemistry/ Immunofluorescence - Anti-NQO1 antibody (ab2346)ICC/IF image of ab2346 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2346, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
参考文献
ab2346 は 69 報の論文で使用されています。
- Salama RM et al. LCZ696 (sacubitril/valsartan) protects against cyclophosphamide-induced testicular toxicity in rats: Role of neprilysin inhibition and lncRNA TUG1 in ameliorating apoptosis. Toxicology 437:152439 (2020). PubMed: 32197949
- Huang C et al. TLR3 Ligand PolyI:C Prevents Acute Pancreatitis Through the Interferon-ß/Interferon-a/ß Receptor Signaling Pathway in a Caerulein-Induced Pancreatitis Mouse Model. Front Immunol 10:980 (2019). PubMed: 31130960
- Guo H et al. Apigetrin treatment attenuates LPS-induced acute otitis media though suppressing inflammation and oxidative stress. Biomed Pharmacother 109:1978-1987 (2019). PubMed: 30551453
- Yagishita Y et al. Nrf2 represses the onset of type 1 diabetes in non-obese diabetic mice. J Endocrinol N/A:N/A (2019). PubMed: 30625116
- Xiao X et al. Biogenic nanoselenium particles activate Nrf2-ARE pathway by phosphorylating p38, ERK1/2, and AKT on IPEC-J2 cells. J Cell Physiol 234:11227-11234 (2019). PubMed: 30488492
