Anti-NPTN 抗体 (ab272652)
Key features and details
- Rabbit polyclonal to NPTN
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-NPTN antibody -
製品の詳細
Rabbit polyclonal to NPTN -
由来種
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
-
免疫原
Recombinant fragment corresponding to Human NPTN aa 200-350.
Database link: Q9Y639 -
ポジティブ･コントロール
- WB: A-549 cells. IHC-P: Human cerebellum tissue. IF: MCF7 cells.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272652 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| WB |
Human
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
|
| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
|
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ターゲット情報
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機能
Probable homophilic and heterophilic cell adhesion molecule involved in long term potentiation at hippocampal excitatory synapses through activation of p38MAPK. May also regulate neurite outgrowth by activating the FGFR1 signaling pathway. May play a role in synaptic plasticity. -
組織特異性
Isoform 1 is ubiquitously expressed. Isoform 2 is expressed in brain cortex and cerebellum (at protein level). -
配列類似性
Contains 3 Ig-like (immunoglobulin-like) domains. -
ドメイン
Some isoforms lack the first Ig-like domain which may confer homophilic adhesion activity. However, they can bind and activate FGFR1. -
翻訳後修飾
N-glycosylated. -
細胞内局在
Cell membrane. Enriched at post-synaptic density. - Information by UniProt
-
参照データベース
- Entrez Gene: 27020 Human
- Omim: 612820 Human
- SwissProt: Q9Y639 Human
- Unigene: 713221 Human
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別名
- GP55 antibody
- GP65 antibody
- Neuroplastin antibody
see all
画像
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Western blot - Anti-NPTN antibody (ab272652)Lane 2 : Anti-NPTN antibody (ab272652) at 0.4 µg/ml
Lane 1 : Molecular Weight ladder
Lane 2 : A-549 cells (Human lung carcinoma cell line) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPTN antibody (ab272652)Paraffin-embedded formalin-fixed human cerebellum tissue stained for NPTN using ab272652 at 1/50 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-NPTN antibody (ab272652)PFA fixed, Triton X-100 permeablized MCF7 cells (human breast adenocarcinoma cell line) labeling NPTN (green) using ab272652 at 2 ug/ml in ICC/IF analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab272652 は論文での使用が確認できていません。
