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Anti-NPR-C 抗体 (ab97389)

Reviews (2)Q&A (1)References (2)
Western blot - Anti-NPR-C antibody (ab97389)
  • Immunocytochemistry/ Immunofluorescence - Anti-NPR-C antibody (ab97389)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPR-C antibody (ab97389)

Key features and details

  • Rabbit polyclonal to NPR-C
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-NPR-C antibody [EPR12716] - C-terminal (ab177954)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-NPR-C antibody
    NPR-C 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to NPR-C

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IFmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow

  • 免疫原

    Recombinant fragment corresponding to Human NPR-C aa 265-514.
    Database link: NP_000899

  • ポジティブ･コントロール

    • WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lines IHC-P: U87 xenograft ICC/IF: Hela cell

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab97389の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/500 - 1/3000. Predicted molecular weight: 60 kDa.
IHC-P (1)
1/100 - 1/500.
ICC/IF
1/100 - 1/200.
特記事項
WB
1/500 - 1/3000. Predicted molecular weight: 60 kDa.
IHC-P
1/100 - 1/500.
ICC/IF
1/100 - 1/200.

ターゲット情報

  • 機能

    Receptor for the natriuretic peptide hormones, binding with similar affinities atrial natriuretic peptide NPPA/ANP, brain natriuretic peptide NPPB/BNP, and C-type natriuretic peptide NPPC/CNP. May function as a clearance receptor for NPPA, NPPB and NPPC, regulating their local concentrations and effects. May regulate diuresis, blood pressure and skeletal development. Does not have guanylate cyclase activity.

  • 配列類似性

    Belongs to the ANF receptor family.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • ANP C antibody
    • ANP-C antibody
    • ANPC antibody
    • ANPR-C antibody
    • ANPRC antibody
    • ANPRC_HUMAN antibody
    • Atrial natriuretic peptide C type receptor antibody
    • Atrial natriuretic peptide clearance receptor antibody
    • Atrial natriuretic peptide receptor 3 antibody
    • Atrial natriuretic peptide receptor type C antibody
    • Atrionatriuretic peptide receptor C antibody
    • C5orf23 antibody
    • FLJ14054 antibody
    • GUCY2B antibody
    • MGC22189 antibody
    • Natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptide receptor C) antibody
    • Natriuretic peptide receptor C/guanylate cyclase C antibody
    • NPR 3 antibody
    • NPR C antibody
    • NPR-C antibody
    • Npr3 antibody
    • NPRC antibody
    see all

画像

  • Western blot - Anti-NPR-C antibody (ab97389)
    Western blot - Anti-NPR-C antibody (ab97389)
    Anti-NPR-C antibody (ab97389) at 1/10000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 60 kDa



    7.5% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-NPR-C antibody (ab97389)
    Immunocytochemistry/ Immunofluorescence - Anti-NPR-C antibody (ab97389)

    ab97389, at a 1/200 dilution, staining NPR-C in paraformaldehyde fixed HeLa cell by Immunofluorescence.
    The lower image (Panel 2) was merged with DNA probe.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPR-C antibody (ab97389)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPR-C antibody (ab97389)

    ab97389, at a 1/500 dilution, staining NPR-C in paraffin embedded U87 xenograft by Immunohistochemistry.

参考文献 (2)

ab97389 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab97389 は 2 報の論文で使用されています。

  • Zayed MA  et al. Natriuretic Peptide Receptor-C is Up-Regulated in the Intima of Advanced Carotid Artery Atherosclerosis. J Med Surg Pathol 1:N/A (2016). IHC-P ; Human . PubMed: 27547837
  • Peake N  et al. Natriuretic peptide receptors regulate cytoprotective effects in a human ex vivo 3D/bioreactor model. Arthritis Res Ther 15:R76 (2013). WB, IF . PubMed: 23883591

レビューと Q&A

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1-3 of 3 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Natriuretic Peptide Receptor C antibody

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Placenta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, pH9.0, 20 min, 100C
Permeabilization
No
Specification
Placenta
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Dec 02 2016

Western blot abreview for Anti-Natriuretic Peptide Receptor C antibody

 Average
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Intestinal Myofibroblasts)
Loading amount
30 µg
Specification
Intestinal Myofibroblasts
Gel Running Conditions
Reduced Denaturing (7.5 % gel)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Dec 18 2012

Question

Would like a testing code to try this antibody with mouse samples.

Read More
Answer

DISCOUNT CODE: ABTRIAL-TBSWD
Expiration date: March 1, 2013
Value: $337

I am very pleased to hear you would like to test ab97389 in with mouse samples. This code will give you $337 off your next order before the expiration date. To redeem this offer, please submit an Abreview for ab97389 and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

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