製品の概要

  • 製品名
    Anti-Noxa antibody [114C307]
    Noxa 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [114C307] to Noxa
  • 由来種
    Mouse
  • アプリケーション
    適用あり: Flow Cyt, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Fusion protein containing human Noxa.

  • ポジティブ･コントロール
    • WB: RL-7 cell lysate. Flow Cyt: Jurkat cells. IHC-P: Chronic Lymphocytic Leukemia (CLL) xenograft tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab13654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P Use a concentration of 5 µg/ml.

Bouin-fixed.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 11 kDa.

ターゲット情報

  • 機能
    Promotes activation of caspases and apoptosis. Promotes mitochondrial membrane changes and efflux of apoptogenic proteins from the mitochondria. Contributes to p53/TP53-dependent apoptosis after radiation exposure. Promotes proteasomal degradation of MCL1. Competes with BAK1 for binding to MCL1 and can displace BAK1 from its binding site on MCL1 (By similarity). Competes with BIM/BCL2L11 for binding to MCL1 and can displace BIM/BCL2L11 from its binding site on MCL1.
  • 組織特異性
    Highly expressed in adult T-cell leukemia cell line.
  • 配列類似性
    Belongs to the PMAIP1 family.
  • ドメイン
    The BH3 motif is essential for pro-apoptotic activity.
  • 細胞内局在
    Mitochondrion.
  • Information by UniProt
  • 参照データベース
    • 別名
      • Adult T cell leukemia derived PMA responsive antibody
      • APR antibody
      • APR_HUMAN antibody
      • ATL-derived antibody
      • Immediate early response protein APR antibody
      • Immediate-early-response protein APR antibody
      • NOXA antibody
      • Phorbol 12 myristate 13 acetate induced protein 1 antibody
      • Phorbol-12-myristate-13-acetate-induced protein 1 antibody
      • PMA induced protein 1 antibody
      • PMA-induced protein 1 antibody
      • PMA-responsive gene antibody
      • Pmaip1 antibody
      • Protein Noxa antibody
      see all

    画像

    • Western blot - Anti-Noxa antibody [114C307] (ab13654)
      Western blot - Anti-Noxa antibody [114C307] (ab13654)

      Western blot detection of Noxa in RL-7 cell (a follicular lymphoma) lysate. A protein of approximate molecular weight of 11 kDa is detected using ab13654 at 2 µg/ml.

      Western blot detection of Noxa in RL-7 cell (a follicular lymphoma) lysate. A protein of approximate molecular weight of 11 kDa is detected using ab13654 at 2 µg/ml.
    • Flow Cytometry - Anti-Noxa antibody [114C307] (ab13654)
      Flow Cytometry - Anti-Noxa antibody [114C307] (ab13654)
      Overlay histogram showing Jurkat cells stained with ab13654 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13654, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions.

    • Immunohistochemistry paraffin embedded sections - Anti-Noxa antibody [114C307] (ab13654)
      Immunohistochemistry paraffin embedded sections - Anti-Noxa antibody [114C307] (ab13654)

      Bouin-fixed, paraffin-embedded human tonsil tissue stained for Chronic Lymphocytic Leukemia (CLL) xenograft tissue stained for Noxa using ab13654 at 1/2000 dilution in immunohistochemical analysis.

    参考文献

    This product has been referenced in:
    • Fitzsimmons L  et al. Coordinated repression of BIM and PUMA by Epstein-Barr virus latent genes maintains the survival of Burkitt lymphoma cells. Cell Death Differ 25:241-254 (2018). WB . Read more (PubMed: 28960205) »
    • Hepp MI  et al. A Trichostatin A (TSA)/Sp1-mediated mechanism for the regulation of SALL2 tumor suppressor in Jurkat T cells. Biochim Biophys Acta N/A:N/A (2018). Human . Read more (PubMed: 29778644) »

    See all 41 Publications for this product

    ab13654 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    9 reviews or Q&A

    10
    Votes: Highest First

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Noxa antibody [114C307]

     Good
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (U2OS cells)
    Permeabilization
    Yes - Tween-20
    Specification
    U2OS cells
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Acetone
    Read More
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    Username

    Abcam user community

    Verified customer

    投稿 Dec 28 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Noxa antibody [114C307]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Human Stomach Adenocarcinoma)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 6.0 Citric Acid
    Permeabilization
    Yes - Tween-20
    Specification
    Human Stomach Adenocarcinoma
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
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    Username

    Abcam user community

    Verified customer

    投稿 Dec 28 2017

    Western blot abreview for Anti-Noxa antibody [114C307]

     Excellent
    Abreviews
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (18% gel)
    Sample
    Human Cell lysate - whole cell (Breast)
    Specification
    Breast
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More
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    Abcam user community

    Verified customer

    投稿 Jan 02 2014

    WB with mouse B-cells
    faint band at 11 kDa, but lots of stronger non-specific bands (e.g. at 20 kDa)
    loaded 1 ug protein
    12% gel
    block w/milk
    Ab: 1/1000 overnight 4 degree
    secondary: OK

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

    Read More

    Dear Abcam, I need some information about this product: Anti-Noxa antibody [114C307] (ab13654). I bought this product and you suggest, in the antibody protocol, this: " This antibody gave a positive signal in Jurkat cells fixed with 80% methanol/pe...

    Read More

    Thank you for your enquiry regarding ab13654. Noxa is highly expressed in adult T-cell leukemia cell line. Since Jurkat cells are T cell leukemia cell line, it is a good positive control for studying Noxa. Under the References section of Swiss-Pr...

    Read More

    I am writing this e-mail to you because we have been experiencing problems with your monoclonal antibody to NOXA (Cat N°. ab13654) for several weeks now.  We have tested this antibody on PVDF and nitrocellulose membrane, with 20, 30 and 40 ug of protei...

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    Thank you for contacting us.  I agree that the antibody is not working as expected, and I would be happy to offer you a free of charge replacement, credit, or refund if you have purchased the antibody in the past six months.  It would be very helpful f...

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    Western blot abreview for Anti-Noxa antibody [114C307]

     Average
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Myeloma)
    Loading amount
    30 µg
    Specification
    Myeloma
    Treatment
    1.25 uM RITA for 6 hrs
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Read More
    Username

    Manujendra

    Verified customer

    投稿 Jan 07 2011

    LOT NUMBER 815787 ORDER NUMBER PR862521 DESCRIPTION OF THE PROBLEM Non-specific band, and high background SAMPLE Human multiple myeloma cell lines PRIMARY ANTIBODY Mouse monoclonal to Noxa (ab1...

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    Thank you for your enquiry. Here are some comments and suggestions: 1. You mention using a high dilution of the secondary antibody? What dilution did you use? 2. Further dilution of the primary antibody, perhaps to 0.5ug/ml or 0.25ug/ml, will ...

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    Western blot abreview for Anti-Noxa antibody [114C307]

     Average
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HL60)
    Loading amount
    20 µg
    Specification
    HL60
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
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    Username

    Abcam user community

    Verified customer

    投稿 Jun 26 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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