Anti-Noxa 抗体 [114C307] (ab13654)
Key features and details
- Mouse monoclonal [114C307] to Noxa
- Suitable for: IHC-P, WB, Flow Cyt (Intra)
- Reacts with: Mouse, Human
- Isotype: IgG1
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Noxa antibody [114C307]
Noxa 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [114C307] to Noxa -
由来種
Mouse -
アプリケーション
適用あり: IHC-P, WB, Flow Cyt (Intra)more details -
種交差性
交差種: Mouse, Human -
免疫原
Fusion protein containing human Noxa.
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ポジティブ・コントロール
- WB: RL-7 cell lysate. Flow Cyt (Intra): Jurkat cells. IHC-P: Chronic Lymphocytic Leukemia (CLL) xenograft tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
114C307 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab13654の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 5 µg/ml.
Bouin-fixed. |
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WB | (4) |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 11 kDa.
|
Flow Cyt (Intra) |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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IHC-P
Use a concentration of 5 µg/ml. Bouin-fixed. |
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 11 kDa. |
Flow Cyt (Intra)
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Promotes activation of caspases and apoptosis. Promotes mitochondrial membrane changes and efflux of apoptogenic proteins from the mitochondria. Contributes to p53/TP53-dependent apoptosis after radiation exposure. Promotes proteasomal degradation of MCL1. Competes with BAK1 for binding to MCL1 and can displace BAK1 from its binding site on MCL1 (By similarity). Competes with BIM/BCL2L11 for binding to MCL1 and can displace BIM/BCL2L11 from its binding site on MCL1. -
組織特異性
Highly expressed in adult T-cell leukemia cell line. -
配列類似性
Belongs to the PMAIP1 family. -
ドメイン
The BH3 motif is essential for pro-apoptotic activity. -
細胞内局在
Mitochondrion. - Information by UniProt
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参照データベース
- Entrez Gene: 5366 Human
- Entrez Gene: 58801 Mouse
- Omim: 604959 Human
- SwissProt: Q13794 Human
- SwissProt: Q9JM54 Mouse
- Unigene: 96 Human
- Unigene: 271878 Mouse
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別名
- Adult T cell leukemia derived PMA responsive antibody
- APR antibody
- APR_HUMAN antibody
see all
画像
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Western blot detection of Noxa in RL-7 cell (a follicular lymphoma) lysate. A protein of approximate molecular weight of 11 kDa is detected using ab13654 at 2
Western blot detection of Noxa in RL-7 cell (a follicular lymphoma) lysate. A protein of approximate molecular weight of 11 kDa is detected using ab13654 at 2 µg/ml.µ g/ml. -
Overlay histogram showing Jurkat cells stained with ab13654 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13654, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions.
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Bouin-fixed, paraffin-embedded human tonsil tissue stained for Chronic Lymphocytic Leukemia (CLL) xenograft tissue stained for Noxa using ab13654 at 1/2000 dilution in immunohistochemical analysis.
データシートおよび資料
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Datasheet download
参考文献 (78)
ab13654 は 78 報の論文で使用されています。
- Park SA et al. Role of the SIRT1/p53 regulatory axis in oxidative stress-mediated granulosa cell apoptosis. Mol Med Rep 23:N/A (2021). PubMed: 33179088
- Xu S et al. Cullin-5 neddylation-mediated NOXA degradation is enhanced by PRDX1 oligomers in colorectal cancer. Cell Death Dis 12:265 (2021). PubMed: 33712558
- Latif AL et al. BRD4-mediated repression of p53 is a target for combination therapy in AML. Nat Commun 12:241 (2021). PubMed: 33431824
- Ochiiwa H et al. TAS4464, a NEDD8-activating enzyme inhibitor, activates both intrinsic and extrinsic apoptotic pathways via c-Myc-mediated regulation in acute myeloid leukemia. Oncogene 40:1217-1230 (2021). PubMed: 33420360
- Zhu R et al. FLT3 tyrosine kinase inhibitors synergize with BCL-2 inhibition to eliminate FLT3/ITD acute leukemia cells through BIM activation. Signal Transduct Target Ther 6:186 (2021). PubMed: 34024909