Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Liver progenitor)
Permeabilization
Yes - Triton X-100
Specification
Liver progenitor
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100
Secondary antibody
Dilution
1/50
Name
Abcam antibody:Donkey Anti-Rabbit IgG H&L (DyLight 488) preadsorbed
Additional data
Additional Notes
Cells were treated with TGFÞ1, which is thought to activate Notch signaling in these cells, at 1.5 ng/ml for 3 days.
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投稿 Jul 21 2017