Normal Rabbit Serum (Sterile) (ab7487)

Reviews (2)Q&A (12)References (8)

製品の概要

  • 製品名

    Normal Rabbit Serum (Sterile)
    Rabbit serum ihc 試薬 製品一覧

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Blocking, IHC-P, IHC-Frmore details

  • 特記事項

    Sterile rabbit serum ab7487 for blocking in antibody-based applications or use as an antibody diluent.

    Origin: North America.

    Total Protein Concentration : 78 mg/ml (by Refractometry). Strain: New Zealand White. Sex: Mixed sex

    Raised in: Rabbit

    Purity: Whole antiserum

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab7487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Blocking Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (8)

ab7487 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab7487 は 8 報の論文で使用されています。

  • Xu Y  et al. Differentiation of CD45-/CD31+ lung side population cells into endothelial and smooth muscle cells in vitro. Int J Mol Med 43:1128-1138 (2019). PubMed: 30628669
  • Tie D  et al. Bullous Pemphigoid IgG Induces Cell Dysfunction and Enhances the Motility of Epidermal Keratinocytes via Rac1/Proteasome Activation. Front Immunol 10:200 (2019). PubMed: 30809225
  • Li BQ  et al. WT1-associated protein is a novel prognostic factor in pancreatic ductal adenocarcinoma. Oncol Lett 13:2531-2538 (2017). Blocking . PubMed: 28454430
  • Wu M  et al. Aleukemic extramedullary T lymphoid/myeloid bilineage hematopoietic and lymphoid malignancy with progression to bilineage leukemia at relapse: A case report. Oncol Lett 14:7723-7732 (2017). PubMed: 29344217
  • Zhang M  et al. Expression levels of seprase/FAPa and DPPIV/CD26 in epithelial ovarian carcinoma. Oncol Lett 10:34-42 (2015). PubMed: 26170973
  • Hicks MR  et al. Mechanical strain applied to human fibroblasts differentially regulates skeletal myoblast differentiation. J Appl Physiol 113:465-72 (2012). PubMed: 22678963
  • Marvin J  et al. Normal bone marrow signal-transduction profiles: a requisite for enhanced detection of signaling dysregulations in AML. Blood 117:e120-30 (2011). PubMed: 21233314
  • Shi X  et al. Fhl2 interacts with Foxk1 and corepresses Foxo4 activity in myogenic progenitors. Stem Cells 28:462-9 (2010). Blocking . PubMed: 20013826

レビューと Q&A

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1-10 of 14 Abreviews or Q&A

sterile serum for blocking

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
good for blocking variability of immunofluorescent staining.
5-10% dilution for 1 hour in RT is recommended.

Abcam user community

Verified customer

投稿 Nov 11 2019

very effective blocking

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
I use it to effectively block in IHC-P staining, usually 10% serum in TBST is sufficient.

Abcam user community

Verified customer

投稿 Jul 04 2019

Question

Product code: 7487
Lot number: GR 75104-2
Inquiry: We have received the serum and have aliqoted it and stored it at -20C as recommended on the data sheet. But wondered what the recommended storage length (i.e. to be able to establish expiry dates as we have to put one on them) of the frozen serum would be and if there are any general recommendations for expiries of serum/antibodies. Also please could you let me know what the recommended disposal route of serum would be. Thanks

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Answer

Thank you for contacting us.

Generally the serum is OK for 1 year at 4C and for 10 years when stored at -20C.

Please check the waste disposal policy of your organization. We do not include this information in MSDS, this should be taken care of by the laboratory where these substances are being used.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Question

Dear All,



Sending you attached a list of the tendered items. You can see the products at page 3-5. Please give all of the items a check and let us know which items are best available with Abcam.



Early response requested.



Sincere regards,

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Answer

Thank you for your inquiry.

We have a number of products that are comparable with the products on the list you sent us. For future inquiriesI would like to direct you to the search facility on our website (www.abcam.com).

Please enter the name of the protein into the search box at the top of the home page. A list of products will be generated which can be further refined using the filters on the left hand side of the page. Using these filters will help you to identify products that have been tested in the applications and species you are interested in.

Please find a list of the product code and the links to the respective datasheets below:

ab1012 (Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade)

https://www.abcam.com/index.html?datasheet=1012 (or use the following: https://www.abcam.com/index.html?datasheet=1012).

ab12209 (Anti-Histone H3 (tri methyl K4) antibody [mAbcam12209] - ChIP Grade)

https://www.abcam.com/index.html?datasheet=12209 (or use the following: https://www.abcam.com/index.html?datasheet=12209).

ab6002 (Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade)

https://www.abcam.com/index.html?datasheet=6002 (or use the following: https://www.abcam.com/index.html?datasheet=6002).

ab124462 (Anti-Flag Tag antibody)

https://www.abcam.com/index.html?datasheet=124462 (or use the following: https://www.abcam.com/index.html?datasheet=124462).

ab6939 (Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy3 ®), pre-adsorbed)

https://www.abcam.com/index.html?datasheet=6939 (or use the following: https://www.abcam.com/index.html?datasheet=6939).

ab96899 (Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed)

https://www.abcam.com/index.html?datasheet=96899 (or use the following: https://www.abcam.com/index.html?datasheet=96899).

ab96919 (Donkey anti-Rabbit IgG H&L (DyLight® 488) secondary antibody)

https://www.abcam.com/index.html?datasheet=96919 (or use the following: https://www.abcam.com/index.html?datasheet=96919).

ab96879 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 488), pre-adsorbed)

https://www.abcam.com/index.html?datasheet=96879 (or use the following: https://www.abcam.com/index.html?datasheet=96879).

ab96871 (Goat anti-Mouse IgG H&L (DyLight® 488) secondary antibody)

https://www.abcam.com/index.html?datasheet=96871 (or use the following: https://www.abcam.com/index.html?datasheet=96871).

ab97265 (Goat anti-Mouse IgG Fc (HRP) secondary antibody)

https://www.abcam.com/index.html?datasheet=97265 (or use the following: https://www.abcam.com/index.html?datasheet=97265).

ab7481 (Goat Serum)

https://www.abcam.com/index.html?datasheet=7481 (or use the following: https://www.abcam.com/index.html?datasheet=7481).

ab7487 (Rabbit Serum (sterile))

https://www.abcam.com/index.html?datasheet=7487 (or use the following: https://www.abcam.com/index.html?datasheet=7487).

ab46666 (Herring sperm ssDNA)

https://www.abcam.com/index.html?datasheet=46666 (or use the following: https://www.abcam.com/index.html?datasheet=46666).

ab52579 (Ribonuclease A protein (Active))

https://www.abcam.com/index.html?datasheet=52579 (or use the following: https://www.abcam.com/index.html?datasheet=52579).

ab73430 (DNase I protein (Active))

https://www.abcam.com/index.html?datasheet=73430 (or use the following: https://www.abcam.com/index.html?datasheet=73430).

ab64220 (Proteinase K)

https://www.abcam.com/index.html?datasheet=64220 (or use the following: https://www.abcam.com/index.html?datasheet=64220).

ab65621 (Protease Inhibitor Cocktail)

https://www.abcam.com/index.html?datasheet=65621 (or use the following: https://www.abcam.com/index.html?datasheet=65621).

ab116028 (Prism Ultra Protein Ladder (10-245 kDa))

https://www.abcam.com/index.html?datasheet=116028 (or use the following: https://www.abcam.com/index.html?datasheet=116028).


I hope this information is helpful to you. If you have specific questions about the products identified during the search, please do not hesitate to contact me.

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Question

J’aimerais savoir si vous avez les certificat d’analyse des produits suivants:
Rabbit serum ref: ab7487 lot 833644
Donkey serum ref: ab7475 lot: 854038
Sheep serum ref: ab7489 lot: GR66413-1
Merci d’avance pour votre réponse

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Answer



Veuillez trouver ci-joints les certificats d'analyse pour les produits :

Rabbit serum ref : ab7487 lot 833644
Donkey serum ref: ab7475 lot: 854038
Sheep serum ref: ab7489 lot: GR66413-1

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Question

Kindly phoned to ask for a CoC for Ab7487 Lot GR75104-1.

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Answer

Thank you for contacting us.

I have attached the CoC for AB7487 as you have requested.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Our customer needs rabbit serum for the blocking in IP. Kindly advise if ab7487 will be suitable for this purpose.

Thank you.

Kind Regards,

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Answer

Thank you for your inquiry.
Yes, I am happy to confirm that rabbit serum ab7487 can be used for blocking in IP.
I hope this information is helpful. Please let me know if you would need some additional or more specific information.
I wish you a good day!

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Question

Dear Abcam, on the datasheet of these sera I have a concentration of 86mg/ml for the goat serum (8,6% concentration) and 71mg/ml for the rabbit serum (7,1% concentration). So no chance I can use them at 10% for IHC blocking on free-floating sections? 

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Answer

Thank you for contacting Abcam.
Those values are for whole protein value which we supply as a customer service. The total protein value is not considered when making blocking solutions as the percentage used is of the whole serum. Therefore to create a 100 ml or a 10% IHC block you would use 10 ml of serum in 90 ml of diluent.
I hope this information is helpful. Please let me know if you have any questions, concerns or there is any other way that I might help you.

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Question

Dear Abcam,
sorry, I have another request regarding these sera.
In your experience, what is the optimal dilution to use these sera for
blocking in IHC on free-floating sections?
Thank you very much,

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Answer

Thank you for your inquiry.
According to the standard protocol we have for free floating sections, we recommend to incubate the sections with 0.1M PB/0.3% triton/1% blocking serum for at least 1 h.
The whole protocol can be found here:

https://www.abcam.com/index.html?pageconfig=resource&rid=13633

As always certain steps including the amount of blocking agent might have to be optimized according to circumstances like sample, results, etc.
I hope this information is helpful. Please do not hesitate to contact me again with any further questions.

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Question

Dear Abcam,
I would like to know if I need to heat-inactivate goat or both serums
before aliquoting and storing, and the best protocol you suggest do
it. The heat-inactivation is not mentioned in the datasheet, anyway
I would like to be sure about it.
Thank you very much,

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Answer

Thank you for your inquiry.
I assume you would like to use this serum for IHC in the blocking step?
In this case the serum does not have to be heat treated. The heat treatment is done to inactivate complement in FCS meant for cell culture for example.
I recommend to just aliquot and freeze the serum.
I hope this information is helpful. Please do not hesitate to contact me again with any further questions.

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1-10 of 14 Abreviews or Q&A

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