Normal Mouse Serum (sterile) (ab7486)

Submit a review Q&A (13)References (5)

製品の概要

  • 製品名

    Normal Mouse Serum (sterile)

  • 製品の詳細

    Normal Mouse Serum (sterile)

  • 由来種

    Mouse

  • アプリケーション

    適用あり: Blocking, IHC-P, IHC-Frmore details

  • 特記事項

    Mouse serum for blocking in antibody-based applications or use as an antibody diluent.

    Origin: North America.

    Total Protein Concentration : normal
    Strain: Mouse - Mixed
    Sex: Mixed sex
    Raised in: Mouse

    Purity: Whole antiserum

    Sterilization: This product was aseptically filtered through a 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Constituent: Whole serum
  • Concentration information loading...

  • 精製度

    Whole antiserum

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab7486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Blocking Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (5)

ab7486 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab7486 は 5 報の論文で使用されています。

  • Yuan H  et al. Heat-induced radiolabeling and fluorescence labeling of Feraheme nanoparticles for PET/SPECT imaging and flow cytometry. Nat Protoc 13:392-412 (2018). PubMed: 29370158
  • Lai A  et al. Annular puncture with tumor necrosis factor-alpha injection enhances painful behavior with disc degeneration in vivo. Spine J 16:420-31 (2016). PubMed: 26610672
  • Guo Y  et al. PEG-Like Nanoprobes: Multimodal, Pharmacokinetically and Optically Tunable Nanomaterials. PLoS One 9:e95406 (2014). PubMed: 24781778
  • Marvin J  et al. Normal bone marrow signal-transduction profiles: a requisite for enhanced detection of signaling dysregulations in AML. Blood 117:e120-30 (2011). PubMed: 21233314
  • Im E  et al. Corticotropin-releasing hormone family of peptides regulates intestinal angiogenesis. Gastroenterology 138:2457-67, 2467.e1-5 (2010). PubMed: 20206175

レビューと Q&A

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Question

Article: AB7486
Decription: MOUSE SERUM (STERILE)
Since it an animal by product. We need to know the country of origin of the
animal used for the product.
Kindly support us for further process

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Answer

This origin of this product is North America.

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Question


Inquiry: Does this product ( ab7486) contain azide as preservative? I am looking for azide -free normal mouse serum. Also do you know the strain of mice that this product is derived from, or is it a pool of multiple strains? Thanks, Vk

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Answer

Thank you for contacting us.


This mouse serum is preservative free and is from mixed strains.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Bonjour
je suis intéressée par l'achat de votre sérum de souris référencé ab 7486.
Pourriez vous me dire si ce sérum contient des conservateurs toxiques pour une utilisation in vivo?
Merci pour votre réponse

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Answer

Merci de nous avoir contactés.

Ce sérum est collecté dans des tubes en plastique et laissé coagulé sur glace pour 20 à 30 minutes. Ensuite le sérum est centrifugé pour séparer le sérum du coagulat. Nous n'avons pas testé ce sérum pour la concentration restante de fibrine. Je peux confirmer que ce sérum ne contient pas des agents ajoutés, comme des agents anti-coagulation ou des conservateurs toxiques.

Le sérum est ensuite filtré 0.22um pour stériliser et a été testé pour confirmer l’absence de bactérie. Ce sérum est obtenu de souris saines sans médication ou antibiotiques.

Par contre ce produit n'a pas été testé pour une utilisation in vivo et donc cette application n’est pas couverte par la garantie Abpromise pour ce produit.

J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

Vous utilisez nos produits? Partagez vos résultats sous forme d'Abreview et recevez en échange des CADEAUX!
https://www.abcam.com/abreviews

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Question

I would like to request the MSDS for this product.

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Answer

Thank you for contacting Abcam.


Attached please find the MSDS you requested.


I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

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Question

Hello, what is the quantity (in grammes in 10 mL) of the product ab7486 Mouse serum?
Thanks you

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Answer

Thank you for your enquiry and interest.

The total protein concentration of this mouse serum (ab7486) usually ranges from 60-90 mg/ml.

If you need any further assistance in the future, please do not hesitate to contact me.

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Question

details of the procedure of the serum preparation.

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Answer

Il y a quelques semaines, vous nous avez appelé pour des informations relatives au sérum de souris, ab7486. https://www.abcam.com/index.html?datasheet=7486 (or use the following: https://www.abcam.com/index.html?datasheet=7486).

Je ne suis pas sûre que ceci vous intéresse toujours, et je m'excuse sincèrement pour ce long délais de réponse.

Je vous transmets ici les informations que j'ai trouvé:

Ce sérum est collecté dans des tubes en plastique et laissé coagulé sur glace pour 20 à 30 minutes. Ensuite le sérum est centrifugé pour séparer le sérum du coagulat. Nous n'avons pas testéce sérum pourla concentration restantede fibrine. Je peux confirmer que ce sérum ne contient pas des agents ajoutées, comme des agents anti-coagulation.

Le sérum est ensuite filtré 0.22um pour stériliser. Ce sérum est obtenue de souris sains sans médication ou antibiotiques.

Encore une fois, toutes mes excuses pour ce délais.

N'hésitez s'il vous plait pas à nous recontacter quand-même si vous avez des autres questions.

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Question

Dear technical team,

Our customer is going to cytotoxicity assay and needs mouse serum to apply to cell culture media.

ab7486 is suitable for this application?

Let me know.

Best regards,

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Answer

Thank you for contacting us.

We have not used the product for the application the customer wishes to use it for but I see no reason why it would not be suitable. The product has been aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers and tested to confirm the absence of bacteria.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

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Question

As Yun has left. I will continue her experiments.  I am wondering if it is possible to exchange another antibody (such as ab13537 DNMT1 antibody) instead of having a new GFP antibody?  

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Answer

Thank you for your email. Yes, a replacement of ab290 with ab13537 is possible. In order to do so, could you please send me the order details (order number, date, name of distributor) ? Thank you.

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Question

Is this serum heat inactivated?

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Answer

Thank you for your telephone enquiry yesterday afternoon. I can confirm that this mouse serum ab7486 has not been heat inactivated. I can recommend if you do need to heat inactivate it to use the following procedure: 1. Thaw the serum slowly to 37°C and mix the contents thoroughly. 2. Place the thawed bottle of serum into a 56°C water bath containing enough water to immerse the vial to just above the level of the serum. 3. Begin timing for 30 minutes. Swirl the serum every 5 to 10 minutes to ensure uniform heating and to prevent protein coagulation. 4. After 30 minutes at 56°C cool the serum immediately, preferrably in an ice bath. I hope this information is helpful. Should you have any further questions, please do not hesitate to contact us.

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Question

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Answer

Thank you for your enquiry. Further to correspondence with the source of this antibody I have determined that the concentration of ab11842 is 0.125 ug/ml. ab11842 is a tissue culture supernatant. The total protein concentration of ab7486 has not been determined but ranges from 80mg - 90mg/mL of serum. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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1-10 of 13 Abreviews or Q&A

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