Normal Goat Serum (ab7481)

Reviews (1)Q&A (10)References (139)
Functional Studies - Normal Goat Serum (ab7481)
  • Functional Studies - Normal Goat Serum (ab7481)

製品の概要

  • 製品名

    Normal Goat Serum
    Goat Serum ihc 試薬 製品一覧

  • 由来種

    Goat

  • アプリケーション

    適用あり: Blocking, IHC-Fr, ICC/IF, IHC-Pmore details

  • 特記事項

    Normal goat serum ab7481 is used extensively for the blocking of non-specific antibody binding in tissue and cell staining, and in other applications of antibodies.

    The goat serum blocks the binding of Fc receptors in the sample to the primary and secondary antibodies used in the experiment, and also blocks non-specific binding of the antibodies to the sample.

    Typically the serum used for blocking is from a different species than the species in which the primary antibody was raised. Often the blocking serum is from the species in which the secondary antibody was raised. 

    Serum can be used directly for blocking, or as a constituent of a blocking buffer.

     

    Strain: Mixed breed and sex.

    Raised in: Goat

    Purity: Whole antiserum

    This product is for research use only and not intended for diagnostic or therapeutic use of any kind. 

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab7481の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Blocking
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent dilution.
IHC-P
Use at an assay dependent dilution.
特記事項
Blocking
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent dilution.
IHC-P
Use at an assay dependent dilution.

画像

  • Functional Studies - Normal Goat Serum (ab7481)
    Functional Studies - Normal Goat Serum (ab7481)Dzinic, Sijana H et al., PloS one vol. 8,11 e74502., Fig 2, doi:10.1371/journal.pone.0074502

    Cells grown in 8-well chamber slides to 70% confluence were fixed with 4% paraformaldehyde (15 min at room temperature (RT)), and permeabilized with 100% ice cold methanol (10 min at 20°C). The slides were incubated with 10% normal goat serum (ab7481) in PBS for 1 hr, and incubated with anti-maspin (1:100) antibody alone or in a combination with either anti-lamin B (1:50), anti-HDAC1 (1:50) or anti-GRP78 (1:50) at 4°C overnight. Cells were washed and incubated for 2 hrs at room temperature (RT) with Alexa Fluor 488 (1:500) alone or in combination with Alexa Fluor 594 (1:500). The nuclei were counterstained with DAPI.
    DU145 cells infected with adenovirus expressing either maspinWT or maspinD346E Confocal immunofluorescence imaging of maspin (green) and nuclear envelope marker lamin B (red) in DU145 cells after infection.

  • Functional Studies - Normal Goat Serum (ab7481)
    Functional Studies - Normal Goat Serum (ab7481)Witasp, Anna et al., PloS one vol. 8,5 e63493., Fig 3, doi:10.1371/journal.pone.0063493
    Slides were pretreated with Hydrogen Peroxide Block followed by 15% normal goat serum (Abcam, Cambridge, UK) for 1 hour. The primary antibody, anti-PTX3, N-terminal antibody produced in rabbit was diluted 1:300 in PBS with 2.5% goat serum (ab7481), applied to slides and incubated for 3 hours at 4°C. The primary antibody was omitted in the negative controls. The PTX3 binding was revealed using a universal secondary antibody polymer formulation conjugated to horseradish peroxidase (HRP). The HRP activity was subsequently visualized with diaminobenzidine (DAB) substrate/chromogen and counterstaining with hematoxylin was performed.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (139)

ab7481 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab7481 は 139 報の論文で使用されています。

  • Jin Q  et al. Allisartan isoproxil attenuates oxidative stress and inflammation through the SIRT1/Nrf2/NF-?B signalling pathway in diabetic cardiomyopathy rats. Mol Med Rep 23:N/A (2021). PubMed: 33495841
  • Meng F  et al. Naloxone Facilitates Contextual Learning and Memory in a Receptor-Independent and Tet1-Dependent Manner. Cell Mol Neurobiol 41:1031-1038 (2021). PubMed: 32989585
  • Alkhalifa H  et al. Inhibition of aquaporins as a potential adjunct to breast cancer cryotherapy. Oncol Lett 21:458 (2021). PubMed: 33907568
  • Yatsenko AS  et al. Exocyst-mediated membrane trafficking of the lissencephaly-associated ECM receptor dystroglycan is required for proper brain compartmentalization. Elife 10:N/A (2021). PubMed: 33620318
  • Yang X  et al. FOXD3-AS1/miR-128-3p/LIMK1 axis regulates cervical cancer progression. Oncol Rep 45:N/A (2021). PubMed: 33760158
View all Publications for this product

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1-10 of 11 Abreviews or Q&A

Perfect for immunostain

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
I've been using this product for a couple of years as a blocking agent before an immunostain of cultured cells and tissue samples.
So far so good. I'll keep using this product.

Mr. Manuel Chacon

Verified customer

投稿 Oct 04 2021

Question

I bought normal goat serum and plan to use it as a blocking reagent before an immunostain of cells and tissue. Should I heat-inactivate the serum before use?

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Answer

There is no need to heat-inactivate serum if you are using it as a blocking reagent before staining fixed cells or tissue. Heat-inactivation is only necessary if you are using the serum for tissue culture. (The heat inactivates complement, and this eliminates complement-mediated cell lysis).

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Question

I'm trying to understand the difference between ab138478and ab7481. I will be using the serum for blocking of IHC on frozen tissue with fluorescent tagged antibodies.

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Answer

ab138478 is sterilized, whereas ab7481 is not. That is the only difference between the two products and the reason that ab138478 is more expensive.

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Question

Kindly advise if ab7481 is guaranteed by Abpromise as a blocking for IP procedure.

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Answer

ab7481 was not experimentally tested as blocking agent in IP procedures. But I am confident that it should be suitable in pre-clearing steps etc in the IP procedure. Please follow this link to a protocol for IP:
https://www.abcam.com/index.html?pageconfig=resource&rid=11385

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Question

On the datasheet of these sera I have a concentration of 86mg/ml for the goat serum (8,6% concentration) and 71mg/ml for the rabbit serum (7,1% concentration). So no chance I can use them at 10% for IHC blocking on free-floating sections? 

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Answer

Those values are for whole protein value which we supply as a customer service. The total protein value is not considered when making blocking solutions as the percentage used is of the whole serum. Therefore to create a 100 ml or a 10% IHC block you would use 10 ml of serum in 90 ml of diluent.

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Question

What is the optimal dilution to use these sera for blocking in IHC on free-floating sections?

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Answer

According to the standard protocol we have for free floating sections, we recommend to incubate the sections with 0.1M PB/0.3% triton/1% blocking serum for at least 1 h.
The whole protocol can be found here:

https://www.abcam.com/index.html?pageconfig=resource&rid=13633

As always certain steps including the amount of blocking agent might have to be optimized according to circumstances like sample, results, etc.

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Question

I would like to know if I need to heat-inactivate goat serum before aliquoting and storing, and the best protocol to do it.

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Answer

To use this serum for IHC in the blocking step, it does not have to be heat treated. The heat treatment is done to inactivate complement in FCS meant for cell culture for example.

I recommend to aliquot and freeze the serum for storage.

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Question

What is the optimal dilution for ab7481?

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Answer

The dilution that works best for your experiment depends on multiple variables so you should do some titrations to find the best dilution. A good starting point for ab7481 is 10%.

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Question

Was this product heat inactivated?

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Answer

ab7481 (Normal Goat Serum) is not heat-inactivated.

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Question

I am using goat serum as a IHC blocking agent. Are there different "grades" of serum and should I be concerned about this?

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Answer

Serums will vary in how they are prepared, but there are no specific "grades" that we are aware of. Please note that when using serum as a blocking agent, there is a chance that the animal may have produced antibodies to the antigen in question and if present, the antibodies may bind to the antigen and prevent the primary antibody from binding. If this happens, just switch blocking agents.

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