Normal Chicken Serum (sterile) (ab7477)

Submit a review Q&A (3)References (2)

製品の概要

  • 製品名

    Normal Chicken Serum (sterile)

  • 製品の詳細

    Normal Chicken Serum (sterile)

  • 由来種

    Chicken

  • アプリケーション

    適用あり: Blocking, IHC-P, IHC-Frmore details

  • 特記事項

    Chicken serum for blocking in antibody-based applications or use as an antibody diluent. From mixed sex mixed strain chickens. Origin: North America.

    Concentration not determined.

    Purity: Whole antiserum

    Sterilization: This product was aseptically filtered through a 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...

  • 精製度

    Whole antiserum

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab7477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Blocking Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (2)

ab7477 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab7477 は 2 報の論文で使用されています。

  • Evanno E  et al. Tri-methylation of H3K79 is decreased in TGF-ß1-induced epithelial-to-mesenchymal transition in lung cancer. Clin Epigenetics 9:80 (2017). PubMed: 28804523
  • Lachke SA  et al. The cell adhesion gene PVRL3 is associated with congenital ocular defects. Hum Genet : (2011). Blocking, IHC-Fr . PubMed: 21769484

レビューと Q&A

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1-3 of 3 Abreviews or Q&A

Question

We currently have a shipment detained by port health and customs, the product in question is the Chicken Serum AB7477, please see below in yellow the questions that was raised by the permit issuing department and please respond to me at your earliest convenience.

Dear Liza,

Thank you for your inquiry.

We currently do not have an import permit for chicken serum. The disease risks will have to be evaluated and an import permit and health certificate drawn up for this product. This will then have to be negotiated with the vet authority in the UK.

Please confirm exactly what use the serum will be imported for (a full description).

Please also provide any information on processing that the serum has undergone – eg. Irradiation, heat treatment etc. If the serum is raw, please provide information on disease testing that it has undergone – bacteria, fungi, mycoplasma etc.

Please also confirm what kind of facility the blood is drawn from: live/dead chickens; abattoirs/laboratories; are the facilities government approved?


Thank you and kind regards,

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Answer

Thank you fory our enquiry which has been forwarded to the Scientific support team. I am sorry it has taken a few days to obtain this information and I appreciate your patience.

I can confirm that this chicken serum ab7477 Chicken Serumis raw sterile frozen serum processed from blooddrawn from healthy animals without apparent signs of disease in USDA inspected facilities. No specific pathogen testing is performed on the serum.

I hope this will be helpful. Please feel free to contact me for additional assistance or if you have any other questions regarding this issue.

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Question

Dear Technical team, Our user would like to order ab7477(Chicken Serum). He wants to confirm that is ab7477 inactivation or activation? Please check this for our user. Thank you for your consderation all the time.. I look forward to hearing from you. Best regards,

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Answer

Thank you for your enquiry. I can confirm that most batches of ab7477 Chicken Serum (sterile) are not heat inactivated. However, a few batches are heat inactivated. If you wish to be sure, I can recommend to contact us with the batch number and we will be pleased to check this for you. I hope this will be helpful. If you have any further questions, please do not hesitate to contact us.

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Question

Anya would like to know the concentration of IgY in this serum preparation so as to use as a control for ab14078 polyclonal

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Answer

Thank you for your enquiry. According to; "Avian IgY Antibody In vitro and in vivo" BY DAVID CARLANDER the IgY concentration in chicken serum is approximately 5-7 mg/ml. Therefore I would like to recommend adusting your control experiments accordingly. http://products.legacyforlife.net/images/IgYCarlander.pdf#search=%22igy%20concentration%20chicken%20serum%22 I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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