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Normal Bovine Calf Serum (sterile) (ab7479)

Submit a review Q&A (2)References (1)

製品の概要

  • 製品名

    Normal Bovine Calf Serum (sterile)

  • 由来種

    Cow

  • アプリケーション

    適用あり: Blocking, IHC-P, IHC-Frmore details

  • 特記事項

    Total Protein Concentration : normal. Strain: Mixed breed and sex

    Raised in: Cow

    Purity: Whole antiserum

    Sterilization: This product was aseptically filtered through a 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab7479の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Blocking
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
特記事項
Blocking
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (1)

ab7479 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab7479 は 1 報の論文で使用されています。

  • Linghu C  et al. Spatial Multiplexing of Fluorescent Reporters for Imaging Signaling Network Dynamics. Cell 183:1682-1698.e24 (2020). PubMed: 33232692

レビューと Q&A

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1-2 of 2 Abreviews or Q&A

Question

What is the country of origin of ab138477 and ab7479?

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Answer

Both of these sera were producted using starting materials of North American origin in USDA inspected facilities.

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Question

I recently bought ab7479 (bovine serum albumin), I will be using it for blockage in western blotting for monoclonal and polyclonal antibodies for phosphorylated antigens. Since I was using BSA in a different (powder) form, I couldnt be sure of the dilution necessary for this product. There is no data on the protein amount in the datasheet. WB protocol that I follow recommends to use 5% weight to volume preparation for the powder form. What is the recommended amount for this product? Thank you very much.

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Answer

Thank you for your enquiry. The product ab7479 is not bovine serum albumin but bovine serum therefore it contains more proteins that just albumin. This product is typically used to reduce non-specific background staining which may be caused by a bovine secondary antibody during immunohistochemistry staining procedures. It may however work in western blotting. We typically recommend a 10% dilution (10ml serum plus 90ml PBST) as blocking agent for immunohistochemistry so I would recommend trying 5% and 10% (in TBST rather than PBST) and a one hour incubation. Please let me know if you need further help,

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