Anti-Nonspecific Cytotoxic Cells 抗体 [5C.6] (ab2778)

Submit an Abreview Q&A (2)Specific References (1)

製品の概要

  • 製品名
    Anti-Nonspecific Cytotoxic Cells antibody [5C.6]
  • 製品の詳細
    Mouse monoclonal [5C.6] to Nonspecific Cytotoxic Cells
  • 由来種
    Mouse
  • アプリケーション
    適用あり: ELISA, WB, Flow Cyt, Inhibition Assay, IPmore details
  • 種交差性
    交差種: Rat, Horse, Cow, Dog, Pig, Tilapia, Catfish
  • 免疫原

    Other Immunogen Type corresponding to Nonspecific Cytotoxic Cells. Purified NCC cells from catfish.

  • ポジティブ･コントロール
    • This antibody as been successfully used in cytotoxic inhibition studies with NCC/NK/LAK cells, panning, and ELISA procedures. In cytotoxicity assays, this antibody is used under saturating conditions to inhibit target cell conjugation.
  • 特記事項

    U.S. Patent Numbers: 5,028,424 and 5,229,494.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.05% Sodium azide
    Constituent: 0.1% BSA
  • Concentration information loading...
  • 精製度
    Purified IgM
  • 一次抗体 備考
    Non-specific cytotoxic cells (NCCs) in teleosts and their evolutionary homologue are a subpopulation of lymphocytes with properties that distinguish them from either B- or T-cells. One such property is that NCC/natural killer (NK)/lymphokine activated killer (LAK) cells express spontaneous, non-major histocompatibility complex restricted cytotoxic activity. NCC and LAK lyse a variety of transformed murine and human B-cell, T-cell and myeloid targets. A 32 kDa membrane protein [non-specific cytotoxic cell receptor protein (NCCRP-1)] expressed by NCC and certain mammalian NK/LAK cells mediates this cytotoxicity. NCCRP-1 is evolutionarily conserved and is found in species ranging from marine and freshwater teleosts to higher mammals.
  • ポリ/モノ
    モノクローナル
  • クローン名
    5C.6
  • アイソタイプ
    IgM
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab2778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
WB Use a concentration of 10 µg/ml.

By Western blot, this antibody detects a 32 kDa protein representing nonspecific cytotoxic cell receptor protein.
Flow Cyt Use at an assay dependent concentration.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

 
Inhibition Assay Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

参考文献

This product has been referenced in:
  • Jaso-Friedmann L  et al. NCCRP-1: a novel receptor protein sequenced from teleost nonspecific cytotoxic cells. Mol Immunol 34:955-65 (1997). Read more (PubMed: 9464530) »

See 1 Publication for this product

ab2778 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

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2 reviews or Q&A

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using horse peripheral lymphocytes sees no signal used 1 ug Ab for 10^6 cells in 100 uL used controls to show that flow cytometry is working What concentration and buffer was successfully tested?

Thank you for contacting us. I received the following information from the lab: We don’t have the specific protocol used to test this antibody in flow cytometry. This product was developed outside our lab, and upon checking our files, I ...

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Hi, I want ues your products ab 2778 and ab37131 in ELISA method. Please inform me how can i prepare a standard curve for the concerned proteins to quantify them? Do you also have the standard proteins concerned with the above antibodies? Please let me...

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I have been informed by the suppliers for ab37131 and ab2778 that we do not supply the standard proteins unfortunately. For ab2778, anti-Nonspecific Cytotoxic Cell antibody, the lab team has informed me that this antibody was actually tested in ELIS...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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