Western blot abreview for Anti-non-muscle Myosin IIB/MYH10 antibody [3H2]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HepG2 Cells)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Loading amount
20 µg
Specification
HepG2 Cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
3 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA in PBS
Dilution
1/2000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Donkey anti-Mouse
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 250 kDa Non-specific: Between 130 and 95 kDa

Dr. Armen Petrosyan

Verified customer

投稿 Dec 21 2016

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