製品の概要

  • 製品名

    Anti-non-muscle Myosin IIB/MYH10 antibody [3H2]
    non-muscle Myosin IIB/MYH10 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [3H2] to non-muscle Myosin IIB/MYH10
  • 由来種

    Mouse
  • 特異性

    This antibody reacts with non-muscle myosin heavy chain (SMemb).
  • アプリケーション

    適用あり: IHC-P, ICC/IF, WB, IHC-Fr, Flow Cytmore details
  • 種交差性

    交差種: Mouse, Rat, Rabbit, Hamster, Human
  • 免疫原

    Synthetic peptide corresponding to Human non-muscle Myosin IIB/MYH10 (C terminal).
    Sequence:

    TSDVNETQPPQSE

  • 特記事項

     This product was previously labelled as non-muscle Myosin IIB

     

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Concentration information loading...
  • 精製度

    Ascites
  • ポリ/モノ

    モノクローナル
  • クローン名

    3H2
  • アイソタイプ

    IgG2b
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab684 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100.
ICC/IF Use at an assay dependent concentration. PubMed: 17478566Use at an assay dependent dilution.
WB 1/2000.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能

    Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • 組織特異性

    Isoform 1 is expressed in cerebellum and spinal chord. Isoform 2 is expressed in cerebrum and retina. Isoform 3 is expressed in the cerebrum and to a much lower extent in cerebellum.
  • 配列類似性

    Contains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • ドメイン

    The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
  • 翻訳後修飾

    Phosphorylated by ABL2.
  • Information by UniProt
  • 参照データベース

  • 別名

    • Cellular myosin heavy chain antibody
    • Cellular myosin heavy chain type B antibody
    • Cellular myosin heavy chain type B type B antibody
    • MGC134913 antibody
    • MGC134914 antibody
    • MYH 10 antibody
    • Myh10 antibody
    • MYH10_HUMAN antibody
    • Myosin 10 antibody
    • Myosin heavy chain 10 antibody
    • Myosin heavy chain 10 non muscle antibody
    • Myosin heavy chain antibody
    • Myosin heavy chain non muscle 11b antibody
    • Myosin heavy chain nonmuscle IIb antibody
    • Myosin heavy chain nonmuscle type B antibody
    • Myosin heavy polypeptide 10 non muscle antibody
    • Myosin-10 antibody
    • Myosin10 antibody
    • NMMHC B antibody
    • NMMHC II b antibody
    • NMMHC II-b antibody
    • NMMHC IIB antibody
    • NMMHC-B antibody
    • NMMHC-IIB antibody
    • NMMHCB antibody
    • Non muscle myosin heavy chain B antibody
    • Non muscle myosin heavy chain IIB antibody
    • Non muscle myosin II heavy chain B antibody
    • non-muscle IIb antibody
    • Non-muscle myosin heavy chain B antibody
    • Non-muscle myosin heavy chain IIb antibody
    • Nonmuscle myosin heavy chain B antibody
    • Nonmuscle myosin heavy chain IIB antibody
    • Nonmuscle myosin II heavy chain B antibody
    • Nonmuscle myosin IIB antibody
    • type B antibody
    see all

画像

  • All lanes : Anti-non-muscle Myosin IIB/MYH10 antibody [3H2] (ab684) at 1/2000 dilution

    All lanes : Human vascular smooth muscle whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated sheep anti-mouse IgG at 1/10000 dilution

    Performed under reducing conditions.

    Observed band size: 200 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    Blocked with 5% milk for 1 hour at 20°C.

    Incubated with the primary antibody in 5% milk for 16 hours at 4°C.

    The 4 lanes were each loaded with 10ug of protein based upon a BCA assay for protein quantification. However, looking at a subsequent loading control blot (vinvulin) lanes 1 and 4 are under loaded versus lanes 2 and 3.

    See Abreview

  • ab684 staining non-muscle Myosin IIB/MYH10 in WIF-B cells (hepatoma-derived hybrid cell line) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 1% donkey serum in 1% PBST for 1 hour at 22°C. Samples were incubated with primary antibody (1/50 in blocking buffer) for 3 hours at 22°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Ab684 staining nnon-muscle Myosin IIB/MYH10 in Human colon.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

  • Overlay histogram showing HeLa cells stained with ab684 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab684, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.

参考文献

This product has been referenced in:

See all 39 Publications for this product

レビューと Q&A

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1-10 of 10 Abreviews

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mice hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100
Specification
Mice hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Dr. Armen Petrosyan

Verified customer

投稿 Jul 22 2019

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mice Hepatocytes)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Loading amount
35 µg
Specification
Mice Hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Armen Petrosyan

Verified customer

投稿 Feb 18 2019

Application
Western blot
Sample
Human Cell lysate - whole cell (HepG2 Cells)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Loading amount
20 µg
Specification
HepG2 Cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Armen Petrosyan

Verified customer

投稿 Dec 21 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Dr. Armen Petrosyan

Verified customer

投稿 Dec 21 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (WIF-B cells, the hepatoma-derived hybrid cell line)
Permeabilization
Yes - 0.2% Triton X-100
Specification
WIF-B cells, the hepatoma-derived hybrid cell line
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Dr. Armen Petrosyan

Verified customer

投稿 Dec 08 2016

Application
Western blot
Sample
Human Cell lysate - other (293T)
Gel Running Conditions
Reduced Denaturing (4-12% SDS-PAGE)
Loading amount
15 µg
Treatment
siRNA transfected for 2 days
Specification
293T
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

投稿 Aug 15 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Aorta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH6
Permeabilization
No
Specification
Aorta
Blocking step
Blocking reagent from ab127055 kit as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde

Miss. Cristina Rodriguez

Verified customer

投稿 Apr 19 2013

Application
Western blot
Sample
Human Cell lysate - whole cell (VSMC)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
VSMC
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Miss. Cristina Rodriguez

Verified customer

投稿 Apr 12 2013

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (embryonic fibroblast)
Permeabilization
Yes - 0.1% Triton
Specification
embryonic fibroblast
Blocking step
DAKO serum free protein blocker as blocking agent for 20 minute(s) · Concentration: 0.25% · Temperature: 28°C
Fixative
Methanol

Dr. J Chai

Verified customer

投稿 Apr 13 2012

Application
Western blot
Sample
Human Cell lysate - whole cell (vascular smooth muscle cells)
Gel Running Conditions
Reduced Denaturing (Tris-acetate 3-8%)
Loading amount
10 µg
Specification
vascular smooth muscle cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

投稿 Feb 19 2008

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