Anti-NOL3 抗体 (ab2003)

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Western blot - Anti-NOL3 antibody (ab2003)

    Key features and details

    • Rabbit polyclonal to NOL3
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-NOL3 antibody
      NOL3 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to NOL3

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Synthetic peptide corresponding to Human NOL3 aa 2-18 (N terminal).
      Sequence:

      GNAQERPSETIDRERKR


      (Peptide available as ab8372)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • ポジティブ･コントロール

      • HeLa whole cell lysate.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C.

    • バッファー

      pH: 7.2
      Preservative: 0.02% Sodium azide
    • Concentration information loading...

    • 精製度

      Immunogen affinity purified

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab2003の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    1/500.
    特記事項
    WB
    1/500.

    ターゲット情報

    • 機能

      Isoform 1 may be involved in RNA splicing.
      Isoform 2 may inhibit apoptosis.

    • 組織特異性

      Highly expressed in heart and skeletal muscle. Detected at low levels in placenta, liver, kidney and pancreas.

    • 配列類似性

      Contains 1 CARD domain.

    • 細胞内局在

      Cytoplasm and Nucleus > nucleolus.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Apoptosis repressor with CARD antibody
        • ARC antibody
        • Muscle enriched cytoplasmic protein antibody
        • Muscle-enriched cytoplasmic protein antibody
        • MYC antibody
        • MYP antibody
        • Nol3 antibody
        • NOL3_HUMAN antibody
        • NOP antibody
        • Nop30 antibody
        • Nucleolar protein 3 (apoptosis repressor with CARD domain) antibody
        • Nucleolar protein 3 antibody
        • Nucleolar protein of 30 kDa antibody
        see all

      画像

      参考文献 (1)

      ab2003 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab2003 は 1 報の論文で使用されています。

      • Razorenova OV  et al. The apoptosis repressor with a CARD domain (ARC) gene is a direct hypoxia-inducible factor 1 target gene and promotes survival and proliferation of VHL-deficient renal cancer cells. Mol Cell Biol 34:739-51 (2014). WB ; Human . PubMed: 24344197

      レビューと Q&A

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      Question

      I`m wondering if I could use your ARC(NT)- antibody for immunofluorescence staining of tissue sections and second, I`m wondering if you get any cross reactivity on your Western blot? Thank you,

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      Answer

      The anti-ARC (NT) was affinity purified antibody using immunogenic peptide. It was shown very clean in Western bloting using HeLa, mouse and rat tissue lysates. We havn't test immunohistochemistry with this antibody. It worth to try.

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