Anti-NOL3 抗体 (ab2002)

Submit a review Q&A (2)References (1)

Key features and details

  • Rabbit polyclonal to NOL3
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-NOL3 antibody
    NOL3 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to NOL3

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide corresponding to Human NOL3 aa 191-208 (C terminal).
    Sequence:

    PDPEPEPDFEERDESEDS


    (Peptide available as ab8371)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • HeLa whole cell lysate

  • 特記事項

    NOL3 domain

     This product was previously labelled as Apoptosis repressor with CARD

     

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • バッファー

    pH: 7.2
    Preservative: 0.02% Sodium azide
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab2002 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
WB
  • 追加情報
    IHC-P: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Detects a band of approximately 25 kDa. Can be blocked with ARC (C-term) peptide (human).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • ターゲット情報

    • 機能

      Isoform 1 may be involved in RNA splicing.
      Isoform 2 may inhibit apoptosis.

    • 組織特異性

      Highly expressed in heart and skeletal muscle. Detected at low levels in placenta, liver, kidney and pancreas.

    • 配列類似性

      Contains 1 CARD domain.

    • 細胞内局在

      Cytoplasm and Nucleus > nucleolus.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Apoptosis repressor with CARD antibody
        • ARC antibody
        • Muscle enriched cytoplasmic protein antibody
        • Muscle-enriched cytoplasmic protein antibody
        • MYC antibody
        • MYP antibody
        • Nol3 antibody
        • NOL3_HUMAN antibody
        • NOP antibody
        • Nop30 antibody
        • Nucleolar protein 3 (apoptosis repressor with CARD domain) antibody
        • Nucleolar protein 3 antibody
        • Nucleolar protein of 30 kDa antibody
        see all

      画像

      • Western blot - Anti-NOL3 antibody (ab2002)
        Western blot - Anti-NOL3 antibody (ab2002)
        All lanes : Anti-NOL3 antibody (ab2002) at 1/1000 dilution

        Lane 1 : HeLa whole cell lysate
        Lane 2 : KB whole cell lysate
        Lane 3 : A549 whole cell lysate

        Observed band size: 25 kDa
        why is the actual band size different from the predicted?

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL3 antibody (ab2002)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL3 antibody (ab2002)

        Immunohistochemistry of NOL3 in human heart tissue with Anti-NOL3 (ab2002) at 5 µg/ml.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL3 antibody (ab2002)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL3 antibody (ab2002)

        Immunohistochemistry of NOL3  in skeletal muscle with Anti-NOL3  (ab2002) at 5 µg/ml.

      参考文献 (1)

      ab2002 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab2002 は 1 報の論文で使用されています。

      • Razorenova OV  et al. The apoptosis repressor with a CARD domain (ARC) gene is a direct hypoxia-inducible factor 1 target gene and promotes survival and proliferation of VHL-deficient renal cancer cells. Mol Cell Biol 34:739-51 (2014). WB, IHC-P ; Human . PubMed: 24344197

      レビューと Q&A

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      1-2 of 2 Abreviews or Q&A

      Question

      How are you?

      I thought this may interest Abcam.

      Please see below the response from our customer:

      I have a proposal for your supplier. I am more than happy to write a review in return for a new supply. We have performed both IHC and WB with this antibody and we have fantastic results. By comparison, the IHC images given in your data sheet is not convincing. It look like H&E staining rather than and IHC staining. If your supplier is interested, I am more than happy for further talks.

      I look forward to receiving your response!

      Kind regards,

      Read More
      Answer

      Thank you for forwarding this enquiry.

      Reviewing the IHC images from the datasheet, I understood the customers concerns and contacted the testing laboratory.The images were provided tothem by a collaborator who uses red dyes for detection. In the human heart you can see darker pink/red areas of cytoplasmic staining. However, unfortunately it is difficult to tell with the skeletal muscle tissue. They are going to retest the antibody inIHC with standard DAB detection.

      I am sorry we are not able to offer anything further for this customer on this occasion as the antibody is already tested and covered by our guarantee in IHC-P. We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

      Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet and the customer may like to consider this in return for Abpoint rewards. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

      To find out more about our Abreview system, please see the following link:

      https://www.abcam.com/abreviews

      I hope this information is helpful. Please do not hesitate to contactme again with any other questions.

      Read More

      Question

      Dear Tech support,

      How are you?

      I have a question from a customer and may require your assistance on this please; please see below:

      I am planning to use one of your antibodies (ab 2002-100), nucleolar protein 30 (NOP 30). As you know, NOP30 is the product of the NOL3 gene. Due to alternative splicing, NOL3 produces two protein products, ARC and NOP 30. In the literature, NOL3, ARC and NOP30 are considered synonyms. Your datasheet says the same. Given that the C-terminal region of ARC is proline/glutamic acid (P/E) rich and that of NOP30 is serine/arginine (S/R) rich, I am not sure if it is appropriate to consider both as synonyms. My question, therefore, is what will I be detecting? Is it NOP30 or ARC?

      Please advise!

      Kind regards,

      Read More
      Answer

      Thank you for your enquiry.

      Based on the customers question I would define ab2002Anti-Apoptosis repressor with CARD antibodyas specific to ARC rather than NOP30. Looking at the two isoforms on UniProt the antibody is specific to isoform 2 as listed under O60936.

      I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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