保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
バッファーPreservative: 0.02% Sodium azide
Concentration information loading...
精製度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab2002 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Detects a band of approximately 25 kDa. Can be blocked with ARC (C-term) peptide (human).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
機能Isoform 1 may be involved in RNA splicing.
Isoform 2 may inhibit apoptosis.
組織特異性Highly expressed in heart and skeletal muscle. Detected at low levels in placenta, liver, kidney and pancreas.
配列類似性Contains 1 CARD domain.
細胞内局在Cytoplasm and Nucleus > nucleolus.
- Information by UniProt
- Apoptosis repressor with CARD antibody
- ARC antibody
- Muscle enriched cytoplasmic protein antibody
All lanes : Anti-NOL3 antibody (ab2002) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : KB whole cell lysate
Lane 3 : A549 whole cell lysate
Observed band size: 25 kDa why is the actual band size different from the predicted?
Immunohistochemistry of NOL3 in human heart tissue with Anti-NOL3 (ab2002) at 5 µg/ml.
Immunohistochemistry of NOL3 in skeletal muscle with Anti-NOL3 (ab2002) at 5 µg/ml.
This product has been referenced in:
- Razorenova OV et al. The apoptosis repressor with a CARD domain (ARC) gene is a direct hypoxia-inducible factor 1 target gene and promotes survival and proliferation of VHL-deficient renal cancer cells. Mol Cell Biol 34:739-51 (2014). WB, IHC-P ; Human . Read more (PubMed: 24344197) »