製品の概要

  • 製品名

  • 製品の詳細

    Rabbit polyclonal to NOL3
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: IHC-P, WBmore details
  • 種交差性

    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Human NOL3 aa 191-208 (C terminal).
    Sequence:

    PDPEPEPDFEERDESEDS


    (Peptide available as ab8371)

  • ポジティブ・コントロール

    • HeLa whole cell lysate
  • 特記事項

    NOL3 domain

     This product was previously labelled as Apoptosis repressor with CARD

     

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー

    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab2002 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
WB
  • 追加情報
    IHC-P: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Detects a band of approximately 25 kDa. Can be blocked with ARC (C-term) peptide (human).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能

      Isoform 1 may be involved in RNA splicing.
      Isoform 2 may inhibit apoptosis.
    • 組織特異性

      Highly expressed in heart and skeletal muscle. Detected at low levels in placenta, liver, kidney and pancreas.
    • 配列類似性

      Contains 1 CARD domain.
    • 細胞内局在

      Cytoplasm and Nucleus > nucleolus.
    • Information by UniProt
    • 参照データベース

    • 別名

      • Apoptosis repressor with CARD antibody
      • ARC antibody
      • Muscle enriched cytoplasmic protein antibody
      • Muscle-enriched cytoplasmic protein antibody
      • MYC antibody
      • MYP antibody
      • Nol3 antibody
      • NOL3_HUMAN antibody
      • NOP antibody
      • Nop30 antibody
      • Nucleolar protein 3 (apoptosis repressor with CARD domain) antibody
      • Nucleolar protein 3 antibody
      • Nucleolar protein of 30 kDa antibody
      see all

    画像

    • All lanes : Anti-NOL3 antibody (ab2002) at 1/1000 dilution

      Lane 1 : HeLa whole cell lysate
      Lane 2 : KB whole cell lysate
      Lane 3 : A549 whole cell lysate

      Observed band size: 25 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry of NOL3 in human heart tissue with Anti-NOL3 (ab2002) at 5 µg/ml.

    • Immunohistochemistry of NOL3  in skeletal muscle with Anti-NOL3  (ab2002) at 5 µg/ml.

    参考文献

    This product has been referenced in:

    • Razorenova OV  et al. The apoptosis repressor with a CARD domain (ARC) gene is a direct hypoxia-inducible factor 1 target gene and promotes survival and proliferation of VHL-deficient renal cancer cells. Mol Cell Biol 34:739-51 (2014). WB, IHC-P ; Human . Read more (PubMed: 24344197) »
    See 1 Publication for this product

    レビューと Q&A

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for forwarding this enquiry.

    Reviewing the IHC images from the datasheet, I understood the customers concerns and contacted the testing laboratory.The images were provided tothem by a collaborator who uses red dyes for detection. In the human heart you can see darker pink/red areas of cytoplasmic staining. However, unfortunately it is difficult to tell with the skeletal muscle tissue. They are going to retest the antibody inIHC with standard DAB detection.

    I am sorry we are not able to offer anything further for this customer on this occasion as the antibody is already tested and covered by our guarantee in IHC-P. We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

    Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet and the customer may like to consider this in return for Abpoint rewards. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

    To find out more about our Abreview system, please see the following link:

    https://www.abcam.com/abreviews

    I hope this information is helpful. Please do not hesitate to contactme again with any other questions.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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