Anti-NOL3 抗体 (ab2002)
Key features and details
- Rabbit polyclonal to NOL3
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-NOL3 antibody
NOL3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NOL3 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human NOL3 aa 191-208 (C terminal).
Sequence:PDPEPEPDFEERDESEDS
(Peptide available asab8371) -
ポジティブ・コントロール
- HeLa whole cell lysate
-
特記事項
NOL3 domain
This product was previously labelled as Apoptosis repressor with CARD
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab2002 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P | ||
WB |
WB: Use at an assay dependent dilution. Detects a band of approximately 25 kDa. Can be blocked with ARC (C-term) peptide (human).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
-
機能
Isoform 1 may be involved in RNA splicing.
Isoform 2 may inhibit apoptosis. -
組織特異性
Highly expressed in heart and skeletal muscle. Detected at low levels in placenta, liver, kidney and pancreas. -
配列類似性
Contains 1 CARD domain. -
細胞内局在
Cytoplasm and Nucleus > nucleolus. - Information by UniProt
-
参照データベース
- Entrez Gene: 8996 Human
- Omim: 605235 Human
- SwissProt: O60936 Human
- Unigene: 513667 Human
-
別名
- Apoptosis repressor with CARD antibody
- ARC antibody
- Muscle enriched cytoplasmic protein antibody
see all
画像
-
All lanes : Anti-NOL3 antibody (ab2002) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : KB whole cell lysate
Lane 3 : A549 whole cell lysate
Observed band size: 25 kDa why is the actual band size different from the predicted? -
Immunohistochemistry of NOL3 in human heart tissue with Anti-NOL3 (ab2002) at 5 µg/ml.
-
Immunohistochemistry of NOL3 in skeletal muscle with Anti-NOL3 (ab2002) at 5 µg/ml.
プロトコール
データシートおよび資料
参考文献 (1)
ab2002 は 1 報の論文で使用されています。
- Razorenova OV et al. The apoptosis repressor with a CARD domain (ARC) gene is a direct hypoxia-inducible factor 1 target gene and promotes survival and proliferation of VHL-deficient renal cancer cells. Mol Cell Biol 34:739-51 (2014). WB, IHC-P ; Human . PubMed: 24344197