Anti-Nogo 抗体 [EPR12265] (ab180847)
![Western blot - Anti-Nogo antibody [EPR12265] (ab180847)](https://www.abcam.co.jp/ps/products/180/ab180847/Images/ab180847-210296-anti-nogo-antibody-epr12265-western-blot.jpg "Western blot - Anti-Nogo antibody [EPR12265] (ab180847)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12265] to Nogo
- Suitable for: WB, Flow Cyt (Intra), ICC/IF, IP, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Nogo antibody [EPR12265]
Nogo 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR12265] to Nogo -
由来種
Rabbit -
アプリケーション
適用あり: WB, Flow Cyt (Intra), ICC/IF, IP, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human Nogo aa 100-200 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9NQC3 -
ポジティブ･コントロール
- U87-MG, SH-SY5Y and HeLa whole cell lysate (ab150035). Human brain and breast carcinoma tissues. U87-MG and HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR12265 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab180847の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 130 kDa.
|
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| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| ICC/IF |
1/100 - 1/250.
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| IP |
1/10 - 1/100.
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
The use of an HRP/AP polymerized secondary antibody is recommended. |
| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 130 kDa. |
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
ICC/IF
1/100 - 1/250. |
|
IP
1/10 - 1/100. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended. |
ターゲット情報
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機能
Developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. Regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex (By similarity). Isoform 2 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 inhibit BACE1 activity and amyloid precursor protein processing. -
組織特異性
Isoform 1 is specifically expressed in brain and testis and weakly in heart and skeletal muscle. Isoform 2 is widely expressed except for the liver. Isoform 3 is expressed in brain, skeletal muscle and adipocytes. Isoform 4 is testis-specific. -
配列類似性
Contains 1 reticulon domain. -
ドメイン
Three regions, residues 59-172, 544-725 and the loop 66 amino acids, between the two transmembrane domains, known as Nogo-66 loop, appear to be responsible for the inhibitory effect on neurite outgrowth and the spreading of neurons. This Nogo-66 loop, mediates also the binding of RTN4 to its receptor. -
細胞内局在
Endoplasmic reticulum membrane. Anchored to the membrane of the endoplasmic reticulum through 2 putative transmembrane domains. - Information by UniProt
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参照データベース
- Entrez Gene: 57142 Human
- Omim: 604475 Human
- SwissProt: Q9NQC3 Human
- Unigene: 727710 Human
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別名
- 1110020G17Rik antibody
- AA407876 antibody
- AA409940 antibody
see all
画像
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Western blot - Anti-Nogo antibody [EPR12265] (ab180847)All lanes : Anti-Nogo antibody [EPR12265] (ab180847) at 1/1000 dilution
Lane 1 : U87-MG cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 130 kDa
Observed band size: 40-55 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nogo antibody [EPR12265] (ab180847)](https://www.abcam.co.jp/ps/products/180/ab180847/Images/ab180847-210295-anti-nogo-antibody-epr12265-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nogo antibody [EPR12265] (ab180847)Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human brain tissue labeling Nogo with ab180847 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nogo antibody [EPR12265] (ab180847)](https://www.abcam.co.jp/ps/products/180/ab180847/Images/ab180847-210294-anti-nogo-antibody-epr12265-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nogo antibody [EPR12265] (ab180847)Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human breast carcinoma tissue labeling Nogo with ab180847 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Nogo antibody [EPR12265] (ab180847)](https://www.abcam.co.jp/ps/products/180/ab180847/Images/ab180847-210293-anti-nogo-antibody-epr12265-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Nogo antibody [EPR12265] (ab180847)Immunofluorescence analysis of U87-MG cells labeling Nogo with ab180847 at a 1/100 dilution.
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![Flow Cytometry (Intracellular) - Anti-Nogo antibody [EPR12265] (ab180847)](https://www.abcam.co.jp/ps/products/180/ab180847/Images/ab180847-210292-anti-nogo-antibody-epr12265-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Nogo antibody [EPR12265] (ab180847)Intracellular Flow Cytometry analysis of permeabilized HeLa cells labeling Nogo (red) with ab180847 at a 1/10 dilution, or negative control rabbit IgG (green)
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![Immunoprecipitation - Anti-Nogo antibody [EPR12265] (ab180847)](https://www.abcam.co.jp/ps/products/180/ab180847/Images/ab180847-210291-anti-nogo-antibody-epr12265-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Nogo antibody [EPR12265] (ab180847)Western blot analysis on Immunoprecipitation pellet from either 1) HeLa cell lysate, or 2) 1xPBS (negative control); showing Nogo, using ab180847 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
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![Anti-Nogo antibody [EPR12265] (ab180847)](https://www.abcam.co.jp/ps/products/180/ab180847/Images/ab180847-7-benefits-of-recombinant-antibodies.png)
Anti-Nogo antibody [EPR12265] (ab180847)
プロトコール
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (1)
ab180847 は 1 報の論文で使用されています。
- Liu H et al. Effects of repetitive magnetic stimulation on motor function and GAP43 and 5-HT expression in rats with spinal cord injury. J Int Med Res 48:300060520970765 (2020). WB ; Rat . PubMed: 33356694
