製品の概要

  • 製品名

    Nocodazole, Microtubule inhibitor
  • 製品の詳細

    Microtubule inhibitor
  • 生理活性の詳細

    Microtubule inhibitor. Rapidly depolymerises microtubules in vivo and inhibits tubulin polymerisation in vitro. Inhibits mitosis. Also reduces stem cell pluripotency factors Oct 4 and Nanog.
  • 精製度

    > 98%
  • CAS 番号

    31430-18-9
  • 構造式

    Chemical Structure

製品の特性

  • 体系名

    Methyl [5-(2-thienylcarbonyl)-1H-benzimidazol-2-yl]carbamate
  • 分子量

    301.32
  • 分子式

    C14H11N3O3S
  • PubChem 登録番号

    4122
  • 保存方法

    Store at +4°C. The product can be stored for up to 12 months.
  • 溶解性

    Soluble in DMSO to 50 mM
  • 使用に関する注意

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES 線形表記

    COC(=O)NC1=NC2=C(N1)C=C(C=C2)C(=O)C3=CC=CS3
  • 由来

    Synthetic

画像

  • All lanes : Anti-Vimentin (phospho S56) antibody [EPR21084] (ab217673) at 1/1000 dilution

    Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 100 ng/ml nocodazole (ab120630) for 18 hours, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Observed band size: 57 kDa
    why is the actual band size different from the predicted?


    Exposure time: 23 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Vimentin (phospho S56) was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 100 ng/ml nocodazole (ab120630) for 18 hours, whole cell lysate with ab217673 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab217673 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Lane 1: HeLa treated with 100 ng/ml nocodazole (ab120630) for 18 hours, whole cell lysate 10 μg (Input).
    Lane 2: ab217673 IP in HeLa treated with 100 ng/ml nocodazole (ab120630) for 18 hours, whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab217673 in HeLa treated with 100 ng/ml nocodazole (ab120630) for 18 hours, whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

参考文献

ab120630 has not yet been referenced specifically in any publications.

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