Anti-nNOS (neuronal) 抗体 (ab6175)

Thank you for your reply.

As for ab72428, I will need to check with the lab regarding tested tissue,localization of staining and potential cross-reactivity with eNOS and iNOS. I will letyou know what I find out. Thank you for your patience.

As for references, we list them on the online datasheet in a tab called "Specific references" if we are aware of any. Also, customer reviews can contain testing data on new species and those would be listed in the tab "Abreviews" on the online datasheet.

For ab72428:
References: https://www.abcam.com/nNOS-neuronal-antibody-C-terminal-ab72428-references.html
Abreviews: wehave not yet received Abreviews from other customers for this antibody.

As for ab106417, a rabbit polyclonal, yes, high background due to crossreactivity of the secondary antibody (anti-rabbit) with the endogenous rabbit IgG (just as mouse-on-mouse staining)could potentially pose a problem, but there are ways to get around this.
Here is our mouse-on-mouse staining advice:
https://www.abcam.com/index.html?pageconfig=resource&rid=11465
which can be adapted to rabbit-on-rabbit staining as well.

I hope this information is of some help so far. I will be in touch with more details soon.
In the meantime, please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Thank you for contacting us.

We have 3 antibodies that were tested and are guaranteed (https://www.abcam.com/Abpromise) for IHC-P and rabbit samples: ab1376,ab6175 and ab72428. I would recommend trying one of thosethat were tested as they are covered under our guarantee.
We do not compare antibodies side by side, thus, I have no infomation on which of these antibodies works best in rabbit tissue. If you had unsuccessful experiences with immunogens similar to ab6175 and ab1376, I would suggest to try ab72428.

We have 3 other nNOS antibodies that are tested in IHC-P and predicted to react with rabbit (based on sequence homology) but have not yet been tested in this species:
ab79051, ab106417, ab95436

As for ab3511: This antibody was not tested in IHC-P, thus we do not know how well it would work in this application.

If you decide to try any of the 4 untestedantibodies, you can use our testing discount program. For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein/kit on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody/protein at the full price (100%) of the antibody/protein you have tested, or a full price discount for the amount paid for the kit. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.
This programapplies to ELISA kits and other kits we offer.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

I'm sorry to hear you are experiencing problems with ab6175, I have received feedback from the laboratory that informed me that your protocol is very similar to ours and we would expect it to work, except the addition of tritonx100 in the antibody dilution buffer which significantly helps penetration of the antibody into the tissue. Indeed rat brain is a very good positive control and in our hands the antibody works at 1:2000 (with some background occasionally). This suggests that the antibody has been damaged during shipping or storage. If you purchased the antibody in the last 90 days I can arrange for a replacement vial or refund/credit note if you can provide me with your order details,

All the information available for this product is listed on the on-line datasheet (price, datasheet, publication, suitability, cross-reactivity).

A positive control tissue for this antibody is the thoracic spinal cord (of rat). Pre-ganglionic sympathetic neurons stain for NOS.

We cannot provide a definitive answer to this query. However, it is unlikely that our antibody to bNOS recognizes inducible NOS.

This antibody, ab6175, is known to react with bNOS in rabbits.

Yes the antibody does cross react with mouse.

This anti-NOS antibody has not, to our knowledge, been tested in paraffin sections, but we would be surprised if this antibody did not work in paraffin. It is an antibody that is well suited to immunohistochemistry and we have found it works well for human brain (frozen sections).