Anti-nNOS (neuronal) 抗体 (ab3511)

Submit a review Q&A (2)References (3)
Western blot - Anti-nNOS (neuronal) antibody (ab3511)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody (ab3511)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody (ab3511)
  • Immunohistochemistry (Frozen sections) - Anti-nNOS (neuronal) antibody (ab3511)
  • Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) antibody (ab3511)

Key features and details

  • Rabbit polyclonal to nNOS (neuronal)
  • Suitable for: IHC-Fr, WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-nNOS (neuronal) antibody [EP1855Y] - Low endotoxin, Azide free (ab219373)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-nNOS (neuronal) antibody
    nNOS (neuronal) 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to nNOS (neuronal)

  • 由来種

    Rabbit

  • 特異性

    Detects nNOS from bovine, mouse, rabbit and rat tissues. This antibody does not detect endothelial NOS (eNOS) or inducible NOS (iNOS).

  • アプリケーション

    適用あり: IHC-Fr, WB, IHC-P, ICC/IFmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide corresponding to Rat nNOS (neuronal) aa 700-800.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • WB: Mouse and rat brain tissue lysate. IHC-P: Mouse skeletal muscle tissue, rat cerebellum tissue. IHC-Fr: Rat brain tissue. ICC/IF: SH-SY5Y cells.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab3511の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-Fr
1/800.
WB
1/1000.
IHC-P
1/200 - 1/400.
ICC/IF
Use at an assay dependent concentration.
特記事項
IHC-Fr
1/800.
WB
1/1000.
IHC-P
1/200 - 1/400.
ICC/IF
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.

  • 組織特異性

    Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.

  • 配列類似性

    Belongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
    Contains 1 PDZ (DHR) domain.

  • ドメイン

    The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14.

  • 翻訳後修飾

    Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).

  • 細胞内局在

    Cell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • 2310005C01Rik antibody
    • BNOS antibody
    • Constitutive NOS antibody
    • EC 1.14.13.39 antibody
    • IHPS 1 antibody
    • IHPS1 antibody
    • N-NOS antibody
    • NC-NOS antibody
    • neuronal Nitric Oxide Synthase antibody
    • Neuronal NOS antibody
    • Nitric oxide synthase , neuronal, included antibody
    • Nitric oxide synthase 1 (neuronal) antibody
    • Nitric oxide synthase 1 antibody
    • Nitric oxide synthase, brain antibody
    • Nitric oxide synthase, penile neuronal, included antibody
    • NNOS antibody
    • NO antibody
    • NOS 1 antibody
    • NOS antibody
    • NOS type I antibody
    • NOS-I antibody
    • NOS1 antibody
    • NOS1_HUMAN antibody
    • Peptidyl-cysteine S-nitrosylase NOS1 antibody
    see all

画像

  • Western blot - Anti-nNOS (neuronal) antibody (ab3511)
    Western blot - Anti-nNOS (neuronal) antibody (ab3511)

    Western blot analysis of nNOS was performed by loading 40µg of Mouse (Lane 1) and Rat Brain (Lane 2) tissue lysate onto a 4-12% Bis-Tris polyacrylamide gel. Proteins were transferred to a Nitrocellulose membrane. Membranes were probed with a rabbit polyclonal antibody (ab3511) recognizing nNOS at a dilution of 1:1000.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody (ab3511)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody (ab3511)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse skeletal muscle tissue sections labeling nNOS (neuronal) with ab3511 at 1/400 (left). Antigen retrieval was performed using 10M sodium citrate (pH 6.0) buffer, microwaved for 8-15 minutes. Tissues were blocked in 3% BSA in PBS for 30 minutes at room temperature. A biotin-conjugated secondary antibody was used. Right - negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody (ab3511)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody (ab3511)

    Immunohistochemistry was performed on normal biopsies of deparaffinized Rat cerebellum tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer and microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a Rabbit Polyclonal Antibody recognizing nNOS (ab3511 ) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry (Frozen sections) - Anti-nNOS (neuronal) antibody (ab3511)
    Immunohistochemistry (Frozen sections) - Anti-nNOS (neuronal) antibody (ab3511)

    ab3511 at 1/800 dilution staining nNOS (neuronal) in rat brain tissue section by immunohistochemistry (Frozen sections).

  • Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) antibody (ab3511)
    Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) antibody (ab3511)

    Immunofluorescence analysis of nNOS was done on 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with nNOS Rabbit Polyclonal Antibody (ab3511) at 1/250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin (1/300 dilution). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

参考文献 (3)

ab3511 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab3511 は 3 報の論文で使用されています。

  • Gao Y  et al. Calcium Receptor and Nitric Oxide Synthase Expression in Circular Muscle of Lower Esophagus from Patients with Achalasia. Chin Med J (Engl) 131:2882-2885 (2018). PubMed: 30511698
  • Evers J  et al. Is there a nitrergic modulation of the rat external anal sphincter? Exp Physiol 98:397-404 (2013). ICC/IF ; Rat . PubMed: 22872659
  • Sastre E  et al. Opposite effect of mast cell stabilizers ketotifen and tranilast on the vasoconstrictor response to electrical field stimulation in rat mesenteric artery. PLoS One 8:e73232 (2013). WB . PubMed: 23977380

レビューと Q&A

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1-2 of 2 Abreviews or Q&A

Question

with regard to the antibodies that have been tested and guaranteed (especially for ab72428), what rabbit tissues were used, and what was the localization of the staining? using the same tissue will help to at least confirm the reported reactivity. Aside from your own in-house validation, do you have record of publications that use your antibodies? specifically, are there any that use the antibodies that you've suggested in rabbit tissue IHC-P?

specifically regarding ab72428, the antigen for the antibody is a pentamer from the nNOS c-terminus. with such a short sequence, is there concern for cross-reactivity, especially to iNOS or eNOS, forexample?

thanks for suggesting some other alternative antibodies. Unfortunately ab79051 and ab95436 use a similar antigen to ab6175 and ab1376 (1400-1429 aa region in nNOS), so I would not consider those. ab106417, the last one you suggested, is a rabbit polyclonal. is this an issue when applying the antibody to rabbit tissue?

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Answer

Thank you for your reply.

As for ab72428, I will need to check with the lab regarding tested tissue,localization of staining and potential cross-reactivity with eNOS and iNOS. I will letyou know what I find out. Thank you for your patience.

As for references, we list them on the online datasheet in a tab called "Specific references" if we are aware of any. Also, customer reviews can contain testing data on new species and those would be listed in the tab "Abreviews" on the online datasheet.

For ab72428:
References: https://www.abcam.com/nNOS-neuronal-antibody-C-terminal-ab72428-references.html
Abreviews: wehave not yet received Abreviews from other customers for this antibody.

As for ab106417, a rabbit polyclonal, yes, high background due to crossreactivity of the secondary antibody (anti-rabbit) with the endogenous rabbit IgG (just as mouse-on-mouse staining)could potentially pose a problem, but there are ways to get around this.
Here is our mouse-on-mouse staining advice:
https://www.abcam.com/index.html?pageconfig=resource&rid=11465
which can be adapted to rabbit-on-rabbit staining as well.

I hope this information is of some help so far. I will be in touch with more details soon.
In the meantime, please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

Inquiry: I am interested in purchasing an nNOS antibody for use in immunohistochemistry/immunofluorescence in rabbit paraffin sections. A number of your antibodies in your catalog state they have reactivity with rabbit, and have been documented for use in IHC-P. In particular, I am interested in ab3511 and ab72428. ab6175 and ab1376 meet the criteria, but use a similar antigen to another antibody that I tested and didn't work.
Have any antibodies in your catalog been documented to work specifically for IHC-P in rabbit tissue? which would you recommend that I try?

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Answer

Thank you for contacting us.

We have 3 antibodies that were tested and are guaranteed (https://www.abcam.com/Abpromise) for IHC-P and rabbit samples: ab1376,ab6175 and ab72428. I would recommend trying one of thosethat were tested as they are covered under our guarantee.
We do not compare antibodies side by side, thus, I have no infomation on which of these antibodies works best in rabbit tissue. If you had unsuccessful experiences with immunogens similar to ab6175 and ab1376, I would suggest to try ab72428.

We have 3 other nNOS antibodies that are tested in IHC-P and predicted to react with rabbit (based on sequence homology) but have not yet been tested in this species:
ab79051, ab106417, ab95436

As for ab3511: This antibody was not tested in IHC-P, thus we do not know how well it would work in this application.

If you decide to try any of the 4 untestedantibodies, you can use our testing discount program. For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein/kit on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody/protein at the full price (100%) of the antibody/protein you have tested, or a full price discount for the amount paid for the kit. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.
This programapplies to ELISA kits and other kits we offer.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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