Anti-nNOS (neuronal) 抗体 (ab1376)
Key features and details
- Goat polyclonal to nNOS (neuronal)
- Suitable for: IHC-Fr, IHC-P, IHC-FoFr, WB, ICC, Flow Cyt (Intra), IHC-FrFl, ICC/IF
- Reacts with: Mouse, Rabbit, Human, Common marmoset
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-nNOS (neuronal) antibody
nNOS (neuronal) 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to nNOS (neuronal) -
由来種
Goat -
アプリケーション
適用あり: IHC-Fr, IHC-P, IHC-FoFr, WB, ICC, Flow Cyt (Intra), IHC-FrFl, ICC/IFmore details -
種交差性
交差種: Mouse, Rabbit, Human, Common marmoset
交差が予測される動物種: Cow, Dog
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免疫原
Synthetic peptide: ESKKDTDEVFSS, corresponding to amino acids 1423-1434 of Human NOS1.
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ポジティブ･コントロール
- ICC: HeLa cells. Flow Cyt (intra): KELLY cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab1376の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr | (2) |
Use at an assay dependent concentration. PubMed: 22589245
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| IHC-P | (9) |
Use at an assay dependent concentration. PubMed: 16263087
|
| IHC-FoFr |
Use at an assay dependent concentration.
Ovenight incubation at room temp. |
|
| WB | (4) |
Use at an assay dependent concentration. Predicted molecular weight: 160 kDa.
Lyse in RIPA buffer. |
| ICC |
Use a concentration of 10 µg/ml.
|
|
| Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
|
|
| IHC-FrFl | (1) |
Use at an assay dependent concentration.
|
| ICC/IF |
Use a concentration of 10 µg/ml.
|
| 特記事項 |
|---|
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IHC-Fr
Use at an assay dependent concentration. PubMed: 22589245 |
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IHC-P
Use at an assay dependent concentration. PubMed: 16263087 |
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IHC-FoFr
Use at an assay dependent concentration. Ovenight incubation at room temp. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 160 kDa. Lyse in RIPA buffer. |
|
ICC
Use a concentration of 10 µg/ml. |
|
Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
|
IHC-FrFl
Use at an assay dependent concentration. |
|
ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. -
組織特異性
Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland. -
配列類似性
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain.
Contains 1 PDZ (DHR) domain. -
ドメイン
The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14. -
翻訳後修飾
Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro). -
細胞内局在
Cell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines. - Information by UniProt
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参照データベース
- Entrez Gene: 477498 Dog
- Entrez Gene: 4842 Human
- Entrez Gene: 18125 Mouse
- Entrez Gene: 100009243 Rabbit
- Omim: 163731 Human
- SwissProt: P29475 Human
- SwissProt: Q9Z0J4 Mouse
- SwissProt: O19132 Rabbit
see all -
別名
- 2310005C01Rik antibody
- BNOS antibody
- Constitutive NOS antibody
see all
画像
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Immunocytochemistry - Anti-nNOS (neuronal) antibody (ab1376)
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Flow Cytometry (Intracellular) - Anti-nNOS (neuronal) antibody (ab1376)Flow cytometric analysis of paraformaldehyde fixed KELLY (Human neuroblastoma cell line) cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab1376 for 1 hour (10 µg/ml) followed by Alexa Fluor® 488 secondary antibody (2 µg/ml).
IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.
データシートおよび資料
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Datasheet download
参考文献 (59)
ab1376 は 59 報の論文で使用されています。
- Özdemir-Kumral ZN et al. High-fat Diet Enhances Gastric Contractility, but Abolishes Nesfatin-1-induced Inhibition of Gastric Emptying. J Neurogastroenterol Motil 27:265-278 (2021). PubMed: 33795544
- Abot A et al. Identification of new enterosynes using prebiotics: roles of bioactive lipids and mu-opioid receptor signalling in humans and mice. Gut 70:1078-1087 (2021). PubMed: 33020209
- Yilmaz O et al. An Immunohistochemical Study on the Presence of Nitric Oxide Synthase Isoforms (nNOS, iNOS, eNOS) in the Spinal Cord and Nodose Ganglion of Rats Receiving Ionising Gamma Radiation to their Liver. J Vet Res 64:445-453 (2020). PubMed: 32984637
- Kakizawa S et al. Functional maintenance of calcium store by ShcB adaptor protein in cerebellar Purkinje cells. Sci Rep 10:14475 (2020). PubMed: 32879382
- Masugi-Tokita M et al. Metabotropic Glutamate Receptor Subtype 7 Is Essential for Ejaculation. Mol Neurobiol 57:5208-5218 (2020). PubMed: 32865662
