Anti-nNOS (neuronal) 抗体 (ab1376)
製品の概要
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製品名
Anti-nNOS (neuronal) antibody
nNOS (neuronal) 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to nNOS (neuronal) -
由来種
Goat -
特異性
Preliminary testing was unsuccessful on Rat Brain for the current batch.
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アプリケーション
適用あり: IHC-FrFl, WB, Flow Cyt, IHC-FoFr, IHC-P, IHC-Fr, ICC/IFmore details -
種交差性
交差種: Mouse, Rabbit, Human, Common marmoset
交差が予測される動物種: Cow, Dog -
免疫原
Synthetic peptide: ESKKDTDEVFSS, corresponding to amino acids 1423-1434 of Human NOS1.
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ポジティブ・コントロール
- WB: Human brain tissue lysates. IHC: mouse DRG following spinal nerve ligation (2 weeks). ICC/IF: U2OS cells. Flow Cyt: Kelly cells.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab1376 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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IHC-FrFl | Use at an assay dependent concentration. | |
WB | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 45, 160 kDa (predicted molecular weight: 161 kDa). Lyse in RIPA buffer. |
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Flow Cyt | Use a concentration of 10 µg/ml. | |
IHC-FoFr | 1/300. Ovenight incubation at room temp. | |
IHC-P | Use at an assay dependent concentration. PubMed: 16263087 | |
IHC-Fr | 1/1000. PubMed: 22589245 | |
ICC/IF | Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. -
組織特異性
Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland. -
配列類似性
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain.
Contains 1 PDZ (DHR) domain. -
ドメイン
The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14. -
翻訳後修飾
Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro). -
細胞内局在
Cell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines. - Information by UniProt
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参照データベース
- Entrez Gene: 477498 Dog
- Entrez Gene: 4842 Human
- Entrez Gene: 18125 Mouse
- Entrez Gene: 100009243 Rabbit
- Omim: 163731 Human
- SwissProt: P29475 Human
- SwissProt: Q9Z0J4 Mouse
- SwissProt: O19132 Rabbit
see all -
別名
- 2310005C01Rik antibody
- BNOS antibody
- Constitutive NOS antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody (ab1376)This image is courtesy of an abreview submitted by Carl Hobbs (King's College London, United Kingdom)
Immunohistochemical analysis of foraldehyde-fixed paraffin-embedded marmoset cerebellum tissue sections, labelling nNOS (neuronal) with ab1376 at a dilution of 1/2000 incubated at 21°C for 2 hours in TBS, azide & BSA diluent. Heat mediated antigen retrival was with citric acid. Blocking was with 1% BSA at 21°C for 10 minutes. Secondary was a Horse anti-Goat polyclonal biotin conjugate at 1/300.
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-nNOS (neuronal) antibody (ab1376)This image is courtesy of Sophie Pezet, King's College London, United Kingdomab1376 nNos antibody immunostaining nNos in dorsal root ganglion (DRG) neurons of an animal who had a spinal nerve ligation 2 weeks previous. [A] Image taken X10 objective, [B]Image taken with X20 objective; the staining is restricted to a subpopulation of neurons and is localized in the cytoplasm of these cells; Scale bar=20 microns. ab1376 was used at 1/300 overnight incubation at room temperature on 30 micron sections of PFA perfusion fixed DRG tissue (on slide). Staining visualised using anti goat FITC secondary.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody (ab1376)This image is courtesy of an abreview submitted by Carl Hobbs (King's College London, United Kingdom)
Immunohistochemical analysis of foraldehyde-fixed paraffin-embedded rat cerebral tissue sections, labelling nNOS (neuronal) with ab1376 at a dilution of 1/2000 incubated at 21°C for 2 hours in TBS, azide & BSA diluent. Heat mediated antigen retrival was with citric acid. Blocking was with 1% BSA at 21°C for 10 minutes. Secondary was a Horse anti-Goat polyclonal biotin conjugate at 1/300.
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Immunohistochemistry (Frozen sections) - Anti-nNOS (neuronal) antibody (ab1376)This image is courtesy of an anonymous abreview.
IHC-Fr image of nNOS staining on marmoset caudate sections using ab1376 (1:1000). The tissue was fixed in paraformaldehyde and the sections were then permeabilized using Triton-X. The sections were them blocked using 2% BSA for 1 hour at 20°C. ab1376 was diluted 1:1000 and incubated with the sections for 18 hours at 20°C. The secondary antibody used was HRP conjugated Rabbit polyclonal to Goat IgG (undiluted)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody (ab1376)This image is courtesy of an abreview submitted by Carl Hobbs (King's College London, United Kingdom)
Immunohistochemical analysis of foraldehyde-fixed paraffin-embedded mouse cerebral tissue sections, labelling nNOS (neuronal) with ab1376 at a dilution of 1/2000 incubated at 21°C for 2 hours in TBS, azide & BSA diluent. Heat mediated antigen retrival was with citric acid. Blocking was with 1% BSA at 21°C for 10 minutes. Secondary was a Horse anti-Goat polyclonal biotin conjugate at 1/300.
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Lane 1 : Anti-nNOS (neuronal) antibody (ab1376) at 2 µg/ml
Lane 2 : Anti-nNOS (neuronal) antibody (ab1376) at 1 µg/ml
Lane 1 : Human frontal cortex lysate (35 µg protein in RIPA buffer)
Lane 2 : Human brain sub ventricular zone lysate (35 µg protein in RIPA buffer)
Predicted band size: 161 kDaDetected by chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody (ab1376)This image is courtesy of an Abreview submitted by Carl Hobbs.
Immunohistochemical analysis of foraldehyde-fixed paraffin-embedded dog cerebral tissue sections, labelling nNOS (neuronal) with ab1376 at a dilution of 1/2000 incubated at 21°C for 2 hours in TBS, azide & BSA diluent. Heat mediated antigen retrieval was with citric acid. Blocking was with 1% BSA at 21°C for 10 minutes. Secondary was a Horse anti-Goat polyclonal biotin conjugate at 1/300.
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Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Incuabted with ab1376 for 1 hour (10 µg/ml) followed by Alexa Fluor® 488 secondary antibody (4 µg/ml), showing nuclear and plasma membrane staining. The nuclear stain is DAPI (blue).
Negative control: Unimmunized goat IgG (10 µg/ml) followed by Alexa Fluor® 488 secondary antibody (4 µg/ml).
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Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab1376 for 1 hour (10 µg/ml) followed by Alexa Fluor® 488 secondary antibody (2 µg/ml).
IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.
データシートおよび資料
参考文献
This product has been referenced in:
- Bossolani GDP et al. Comparative analysis reveals Ce3D as optimal clearing method for in toto imaging of the mouse intestine. Neurogastroenterol Motil 31:e13560 (2019). Read more (PubMed: 30761698) »
- Wang S et al. Effects of aerobic exercise on the expressions and activities of nitric oxide synthases in the blood vessel endothelium in prediabetes mellitus. Exp Ther Med 17:4205-4212 (2019). Read more (PubMed: 31007752) »