Anti-nNOS (neuronal) 抗体 (ab1376)
Key features and details
- Goat polyclonal to nNOS (neuronal)
- Suitable for: ICC, Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-nNOS (neuronal) antibody
nNOS (neuronal) 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to nNOS (neuronal) -
由来種
Goat -
アプリケーション
適用あり: ICC, Flow Cyt (Intra)more details -
種交差性
交差種: Human -
免疫原
Synthetic peptide: ESKKDTDEVFSS, corresponding to amino acids 1423-1434 of Human NOS1.
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ポジティブ･コントロール
- ICC: HeLa cells. Flow Cyt (intra): KELLY cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab1376の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC |
Use a concentration of 10 µg/ml.
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| Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
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| 特記事項 |
|---|
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ICC
Use a concentration of 10 µg/ml. |
|
Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. -
組織特異性
Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland. -
配列類似性
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain.
Contains 1 PDZ (DHR) domain. -
ドメイン
The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14. -
翻訳後修飾
Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro). -
細胞内局在
Cell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines. - Information by UniProt
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参照データベース
- Entrez Gene: 4842 Human
- Omim: 163731 Human
- SwissProt: P29475 Human
- Unigene: 654410 Human
- Unigene: 684465 Human
- Unigene: 684466 Human
- Unigene: 684467 Human
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別名
- 2310005C01Rik antibody
- BNOS antibody
- Constitutive NOS antibody
see all
画像
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Immunocytochemistry - Anti-nNOS (neuronal) antibody (ab1376)
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Flow Cytometry (Intracellular) - Anti-nNOS (neuronal) antibody (ab1376)Flow cytometric analysis of paraformaldehyde fixed KELLY (Human neuroblastoma cell line) cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab1376 for 1 hour (10 µg/ml) followed by Alexa Fluor® 488 secondary antibody (2 µg/ml).
IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (60)
ab1376 は 60 報の論文で使用されています。
- Özdemir-Kumral ZN et al. High-fat Diet Enhances Gastric Contractility, but Abolishes Nesfatin-1-induced Inhibition of Gastric Emptying. J Neurogastroenterol Motil 27:265-278 (2021). PubMed: 33795544
- Abot A et al. Identification of new enterosynes using prebiotics: roles of bioactive lipids and mu-opioid receptor signalling in humans and mice. Gut 70:1078-1087 (2021). PubMed: 33020209
- Yilmaz O et al. An Immunohistochemical Study on the Presence of Nitric Oxide Synthase Isoforms (nNOS, iNOS, eNOS) in the Spinal Cord and Nodose Ganglion of Rats Receiving Ionising Gamma Radiation to their Liver. J Vet Res 64:445-453 (2020). PubMed: 32984637
- Kakizawa S et al. Functional maintenance of calcium store by ShcB adaptor protein in cerebellar Purkinje cells. Sci Rep 10:14475 (2020). PubMed: 32879382
- Masugi-Tokita M et al. Metabotropic Glutamate Receptor Subtype 7 Is Essential for Ejaculation. Mol Neurobiol 57:5208-5218 (2020). PubMed: 32865662
