Anti-nNOS (neuronal) 抗体 (ab1376)
Key features and details
- Goat polyclonal to nNOS (neuronal)
- Suitable for: WB, Flow Cyt, IHC-FoFr, ICC/IF
- Reacts with: Mouse, Rabbit
- Isotype: IgG
製品の概要
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製品名
Anti-nNOS (neuronal) antibody
nNOS (neuronal) 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to nNOS (neuronal) -
由来種
Goat -
特異性
Preliminary testing was unsuccessful on Rat Brain for the current batch.
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アプリケーション
適用あり: WB, Flow Cyt, IHC-FoFr, ICC/IFmore details -
種交差性
交差種: Mouse, Rabbit
交差が予測される動物種: Cow, Dog
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免疫原
Synthetic peptide: ESKKDTDEVFSS, corresponding to amino acids 1423-1434 of Human NOS1.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab1376 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 45, 160 kDa (predicted molecular weight: 161 kDa). A 1 hour primary incubation is recommended for this product. Lyse in RIPA buffer. |
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| Flow Cyt | Use a concentration of 10 µg/ml. | |
| IHC-FoFr | 1/300. Ovenight incubation at room temp. | |
| ICC/IF | Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. -
組織特異性
Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland. -
配列類似性
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain.
Contains 1 PDZ (DHR) domain. -
ドメイン
The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14. -
翻訳後修飾
Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro). -
細胞内局在
Cell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines. - Information by UniProt
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参照データベース
- Entrez Gene: 477498 Dog
- Entrez Gene: 18125 Mouse
- Entrez Gene: 100009243 Rabbit
- SwissProt: Q9Z0J4 Mouse
- SwissProt: O19132 Rabbit
- Unigene: 442195 Mouse
- Unigene: 44249 Mouse
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別名
- 2310005C01Rik antibody
- BNOS antibody
- Constitutive NOS antibody
see all
画像
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-nNOS (neuronal) antibody (ab1376)This image is courtesy of Sophie Pezet, King's College London, United Kingdomab1376 nNos antibody immunostaining nNos in dorsal root ganglion (DRG) neurons of an animal who had a spinal nerve ligation 2 weeks previous. [A] Image taken X10 objective, [B]Image taken with X20 objective; the staining is restricted to a subpopulation of neurons and is localized in the cytoplasm of these cells; Scale bar=20 microns. ab1376 was used at 1/300 overnight incubation at room temperature on 30 micron sections of PFA perfusion fixed DRG tissue (on slide). Staining visualised using anti goat FITC secondary. -
Western blot - Anti-nNOS (neuronal) antibody (ab1376)Lane 1 : Anti-nNOS (neuronal) antibody (ab1376) at 2 µg/ml
Lane 2 : Anti-nNOS (neuronal) antibody (ab1376) at 1 µg/ml
Lane 1 : Human frontal cortex lysate (35 µg protein in RIPA buffer)
Lane 2 : Human brain sub ventricular zone lysate (35 µg protein in RIPA buffer)
Predicted band size: 161 kDaDetected by chemiluminescence.
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Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) antibody (ab1376)Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Incuabted with ab1376 for 1 hour (10 µg/ml) followed by Alexa Fluor® 488 secondary antibody (4 µg/ml), showing nuclear and plasma membrane staining. The nuclear stain is DAPI (blue).
Negative control: Unimmunized goat IgG (10 µg/ml) followed by Alexa Fluor® 488 secondary antibody (4 µg/ml).
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Flow Cytometry - Anti-nNOS (neuronal) antibody (ab1376)Flow cytometric analysis of paraformaldehyde fixed KELLY (Human neuroblastoma cell line) cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab1376 for 1 hour (10 µg/ml) followed by Alexa Fluor® 488 secondary antibody (2 µg/ml).
IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.
プロトコール
データシートおよび資料
参考文献 (43)
ab1376 は 43 報の論文で使用されています。
- Moiseev KY et al. Changes of nNOS expression in the tuberal hypothalamic nuclei during ageing. Nitric Oxide 100-101:1-6 (2020). PubMed: 32283261
- Aziz W et al. Multi-input Synapses, but Not LTP-Strengthened Synapses, Correlate with Hippocampal Memory Storage in Aged Mice. Curr Biol 29:3600-3610.e4 (2019). PubMed: 31630953
- Williams RH et al. Excitation of Cortical nNOS/NK1R Neurons by Hypocretin 1 is Independent of Sleep Homeostasis. Cereb Cortex 29:1090-1108 (2019). PubMed: 29462275
- Bossolani GDP et al. Comparative analysis reveals Ce3D as optimal clearing method for in toto imaging of the mouse intestine. Neurogastroenterol Motil 31:e13560 (2019). PubMed: 30761698
- Wang S et al. Effects of aerobic exercise on the expressions and activities of nitric oxide synthases in the blood vessel endothelium in prediabetes mellitus. Exp Ther Med 17:4205-4212 (2019). PubMed: 31007752
