Anti-NMDAR2B (phospho S1480) 抗体 (ab73014)
Key features and details
- Rabbit polyclonal to NMDAR2B (phospho S1480)
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
製品の概要
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製品名
Anti-NMDAR2B (phospho S1480) antibody
NMDAR2B 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NMDAR2B (phospho S1480) -
由来種
Rabbit -
特異性
ab73014 is specific for ~180k NMDAR2B protein phosphorylated at Ser1480. Immunolabeling of the NMDAR2B band is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide. Immunolabeling is also blocked by lambda-phosphatase treatment. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Rat
交差が予測される動物種: Mouse, Chicken, Cow, Dog, Human, Non human primates, Zebrafish -
免疫原
Synthetic peptide corresponding to Rat NMDAR2B (phospho S1480).
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ポジティブ・コントロール
- Rat hippocampal lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.50
Constituents: 0.01% BSA, 50% Glycerol, 0.87% Sodium chloride, 0.238% HEPES -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab73014 is prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab73014の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Detects a band of approximately 180 kDa (predicted molecular weight: 166 kDa).
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特記事項 |
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WB
1/1000. Detects a band of approximately 180 kDa (predicted molecular weight: 166 kDa). |
ターゲット情報
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機能
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. -
組織特異性
Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia. -
配列類似性
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily. -
細胞内局在
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 417965 Chicken
- Entrez Gene: 537804 Cow
- Entrez Gene: 494009 Dog
- Entrez Gene: 2904 Human
- Entrez Gene: 14812 Mouse
- Entrez Gene: 24410 Rat
- Entrez Gene: 100334883 Zebrafish
- Omim: 138252 Human
see all -
別名
- AW490526 antibody
- EIEE27 antibody
- Glutamate [NMDA] receptor subunit epsilon 2 antibody
see all
画像
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All lanes : Anti-NMDAR2B (phospho S1480) antibody (ab73014) at 1/1000 dilution
Lane 1 : Rat hippocampal lysate
Lane 2 : Rat hippocampal lysate incubated in lambda-phosphatase (1200 units for 30 min)
Predicted band size: 166 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Additional bands at: 85 kDa. We are unsure as to the identity of these extra bands.
The phosphospecificity of the labeling is shown in the second lane (lambda-phosphatase). The immunolabeling is completely eliminated by treatment with lambda-phosphatase.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab73014 は 4 報の論文で使用されています。
- Sun Z et al. Casein kinase 2 attenuates brain injury induced by intracerebral hemorrhage via regulation of NR2B phosphorylation. Front Cell Neurosci 16:911973 (2022). PubMed: 35928572
- Zhang C et al. Protopanaxadiol ginsenoside Rd protects against NMDA receptor-mediated excitotoxicity by attenuating calcineurin-regulated DAPK1 activity. Sci Rep 10:8078 (2020). PubMed: 32415270
- McKay S et al. The Developmental Shift of NMDA Receptor Composition Proceeds Independently of GluN2 Subunit-Specific GluN2 C-Terminal Sequences. Cell Rep 25:841-851.e4 (2018). PubMed: 30355491
- Emanuele M et al. Exogenous Alpha-Synuclein Alters Pre- and Post-Synaptic Activity by Fragmenting Lipid Rafts. EBioMedicine 7:191-204 (2016). PubMed: 27322472