Anti-NMDAR2B (phospho S1303) 抗体 [EP1858Y] (ab81271)
![Western blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)](https://www.abcam.co.jp/ps/products/81/ab81271/Images/ab81271-296742-anti-nmdar2b-phospho-s1303-antibody-ep1858y-western-blot.jpg "Western blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1858Y] to NMDAR2B (phospho S1303)
- Suitable for: Dot blot, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-NMDAR2B (phospho S1303) antibody [EP1858Y]
NMDAR2B 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1858Y] to NMDAR2B (phospho S1303) -
由来種
Rabbit -
アプリケーション
適用あり: Dot blot, WBmore details
適用なし: IHC-P or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Human, mouse and rat brain tissue lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1858Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab81271の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Dot blot |
1/1000.
|
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| WB | (1) |
1/1000 - 1/5000. Predicted molecular weight: 166 kDa.
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| 特記事項 |
|---|
|
Dot blot
1/1000. |
|
WB
1/1000 - 1/5000. Predicted molecular weight: 166 kDa. |
ターゲット情報
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機能
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. -
組織特異性
Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia. -
配列類似性
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily. -
細胞内局在
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 2904 Human
- Entrez Gene: 14812 Mouse
- Entrez Gene: 24410 Rat
- Omim: 138252 Human
- SwissProt: Q13224 Human
- SwissProt: Q01097 Mouse
- SwissProt: Q00960 Rat
- Unigene: 654430 Human
see all -
別名
- AW490526 antibody
- EIEE27 antibody
- Glutamate [NMDA] receptor subunit epsilon 2 antibody
see all
画像
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Western blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)All lanes : Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271) at 1/5000 dilution
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat brain tissue lysate. The membrane was incubated with alkaline phosphatase.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 166 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)](https://www.abcam.co.jp/ps/products/81/ab81271/Images/ab81271-296741-anti-nmdar2b-phospho-s1303-antibody-ep1858y-western-blot.jpg)
Western blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)All lanes : Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271) at 1/5000 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : Human brain tissue lysate. The membrane was incubated with alkaline phosphatase.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 166 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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![Dot Blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)](https://www.abcam.co.jp/ps/products/81/ab81271/Images/ab81271-296735-anti-nmdar2b-phospho-s1303-antibody-ep1858y-dot-blot.jpg)
Dot Blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)Dot blot analysis of NMDAR2B (phospho S1303) phospho peptide (Lane 1) and NMDAR2B non-phospho peptide (Lane 2) labelling NMDAR2B (phospho S1303) with ab81271 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000 dilution. Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST.
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![Western blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)](https://www.abcam.co.jp/ps/products/81/ab81271/Images/ab81271-96453-anti-nmdar2b-phospho-s1303-antibody-ep1858y-western-blot.jpg)
Western blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)All lanes : Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271) at 1/5000 dilution
Lane 1 : Mouse brain lysates (untreated)
Lane 2 : Mouse brain lysates (treated with AP)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP labelled IgG at 1/2000 dilution
Predicted band size: 166 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted? -
![Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)](https://www.abcam.co.jp/ps/products/81/ab81271/Images/ab81271-5-benefits-of-recombinant-antibodies.png)
Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (24)
ab81271 は 24 報の論文で使用されています。
- Liu S et al. Icaritin Alleviates Glutamate-Induced Neuronal Damage by Inactivating GluN2B-Containing NMDARs Through the ERK/DAPK1 Pathway. Front Neurosci 15:525615 (2021). PubMed: 33692666
- Gwon DH et al. NFAT5 Deficiency Alleviates Formalin-Induced Inflammatory Pain Through mTOR. Int J Mol Sci 22:N/A (2021). PubMed: 33806698
- Sun W et al. Requirements of Postnatal proBDNF in the Hippocampus for Spatial Memory Consolidation and Neural Function. Front Cell Dev Biol 9:678182 (2021). PubMed: 34336832
- Williams MT et al. Whole brain proton irradiation in adult Sprague Dawley rats produces dose dependent and non-dependent cognitive, behavioral, and dopaminergic effects. Sci Rep 10:21584 (2020). PubMed: 33299021
- Li N et al. LPM580098, a Novel Triple Reuptake Inhibitor of Serotonin, Noradrenaline, and Dopamine, Attenuates Neuropathic Pain. Front Pharmacol 10:53 (2019). PubMed: 30837867