Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (rat neurons)
Permeabilization
Yes - 0,5% Triton
Specification
rat neurons
Blocking step
Duolink blocking solution as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 37°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Duolink Antibody Diluent solution
Dilution
1/2000
Secondary antibody
Dilution
1/5
Name
Non-Abcam antibody was used: Duolink In Situ PLA Probe anti-Mouse MINUS
Host species: Donkey
Clonality: Polyclonal
Conjugation: Other
Host species: Donkey
Clonality: Polyclonal
Conjugation: Other
Additional data
Additional Notes
The antibody was used in PLA method (Proximity Ligation Assay). After incubation with 2ndary antibodies the samples were incubated with Duolink Ligation-Ligase solution for 30 min at 37 °C, followed by washing and incubation with Duolink Amplification-Polymerase solution for 100 min at 37 °C.
On the image the PLA signals, recognized as a fluorescent green dots, show the close proximity of NMDAR2B and STIM2 antigens (complexes).
PubMed ID
31936514: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Mar 18 2020