Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (hippocampus and cortex)
Specification
hippocampus and cortex
Fixative
Paraformaldehyde
Other product details
Concentration
0.5 µg/ml
Incubation time
18 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit alexa fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
This antibody gave a very nice staining in the dendrites of cortical and hippocampal neurons. This staining is quenched by pre-incubation with specific peptide (see figure, panel C). In the spinal cord, it produced a staining somehow different from the staining we are used to obtain with antibodies against NR1 in that staining was also detected in neuron processes of these neurons in addition to the cell body.
Abcam response
Many thanks for your feedback on ab17345. We would advise using a blocking step in your protocol to minimize non-specific binding of the antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Sophie Pezet
Verified customer
投稿 Apr 12 2006