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Anti-NLRP3 抗体 [EPR23073-96] - BSA and Azide free (ab272702)

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Immunocytochemistry - Anti-NLRP3 antibody [EPR23073-96] - BSA and Azide free (ab272702)
  • Immunoprecipitation - Anti-NLRP3 antibody [EPR23073-96] - BSA and Azide free (ab272702)
  • Western blot - Anti-NLRP3 antibody [EPR23073-96] - BSA and Azide free (ab272702)
  • Anti-NLRP3 antibody [EPR23073-96] - BSA and Azide free (ab272702)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23073-96] to NLRP3 - BSA and Azide free
  • Suitable for: ICC, IP, WB
  • Reacts with: Mouse

製品の概要

  • 製品名

    Anti-NLRP3 antibody [EPR23073-96] - BSA and Azide free
    NLRP3 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23073-96] to NLRP3 - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC
    Mouse
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: RAW 264.7 (+/- treatment with LPS) and J774A.1 whole cell lysates. ICC: RAW 264.7 (+ treatment with LPS) cells. IP: RAW 264.7 (+ treatment with LPS) whole cell lysate.

  • 特記事項

    ab272702 is the carrier-free version of ab270449. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
ICC
Mouse
IP
Mouse
WB
Mouse
アプリケーション Abreviews 特記事項
ICC
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).

The level of NLRP3 expression can be elevated when cells are treated with LPS. There may be multiple bands of NLRP3 in WB assay due to different isoforms and PTMs.

Ms Isoform I: 96kD (predicted) Isoform 2-4: 110-120kD (predicted)

Positive Control: Human THP1 WT cells treated with 200 ng/ml LPS for 3h, RAW 264.7 (+/- treatment with LPS) ab7187, Rat spleen lysate.

Negative Control: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate.
特記事項
ICC
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).

The level of NLRP3 expression can be elevated when cells are treated with LPS. There may be multiple bands of NLRP3 in WB assay due to different isoforms and PTMs.

Ms Isoform I: 96kD (predicted) Isoform 2-4: 110-120kD (predicted)

Positive Control: Human THP1 WT cells treated with 200 ng/ml LPS for 3h, RAW 264.7 (+/- treatment with LPS) ab7187, Rat spleen lysate.

Negative Control: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate.
  • 追加情報
    Is unsuitable for Flow Cyt,IHC-Fr or IHC-P.

    • ターゲット情報

    • 機能

      May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18.

    • 組織特異性

      Expressed in blood leukocytes. Strongly expressed in polymorphonuclear cells and osteoblasts. Undetectable or expressed at a lower magnitude in B- and T-lymphoblasts, respectively. High level of expression detected in chondrocytes. Detected in non-keratinizing epithelia of oropharynx, esophagus and ectocervix and in the urothelial layer of the bladder.

    • 関連疾患

      Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1) [MIM:120100]; also known as familial cold urticaria. FCAS are rare autosomal dominant systemic inflammatory diseases characterized by episodes of rash, arthralgia, fever and conjunctivitis after generalized exposure to cold.
      Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS) [MIM:191900]; also known as urticaria-deafness-amyloidosis syndrome. MWS is a hereditary periodic fever syndrome characterized by fever, chronic recurrent urticaria, arthralgias, progressive sensorineural deafness, and reactive renal amyloidosis. The disease may be severe if generalized amyloidosis occurs.
      Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA) [MIM:607115]; also known as neonatal onset multisystem inflammatory disease (NOMID). CINCA is a rare congenital inflammatory disorder characterized by a triad of neonatal onset of cutaneous symptoms, chronic meningitis and joint manifestations with recurrent fever and inflammation.

    • 配列類似性

      Belongs to the NLRP family.
      Contains 1 DAPIN domain.
      Contains 9 LRR (leucine-rich) repeats.
      Contains 1 NACHT domain.

    • 細胞内局在

      Cytoplasm.
    • Information by UniProt

    • 参照データベース

      • 別名

        • AGTAVPRL antibody
        • AII/AVP antibody
        • Angiotensin/vasopressin receptor AII/AVP like antibody
        • Angiotensin/vasopressin receptor AII/AVP-like antibody
        • C1orf7 antibody
        • Caterpiller protein 1.1 antibody
        • CIAS 1 antibody
        • CIAS1 antibody
        • CLR1.1 antibody
        • Cold autoinflammatory syndrome 1 antibody
        • Cold autoinflammatory syndrome 1 protein antibody
        • Cryopyrin antibody
        • Familial cold autoinflammatory syndrome antibody
        • FCAS antibody
        • FCU antibody
        • LRR and PYD domains-containing protein 3 antibody
        • Muckle-Wells syndrome antibody
        • MWS antibody
        • NACHT antibody
        • NACHT LRR and PYD containing protein 3 antibody
        • NALP 3 antibody
        • NALP3 antibody
        • NALP3_HUMAN antibody
        • NLR family pyrin domain containing 3 antibody
        • NLRP3 antibody
        • PYPAF 1 antibody
        • PYPAF1 antibody
        • PYRIN containing APAF1 like protein 1 antibody
        • PYRIN-containing APAF1-like protein 1 antibody
        see all

      画像

      • Immunocytochemistry - Anti-NLRP3 antibody [EPR23073-96] - BSA and Azide free (ab272702)
        Immunocytochemistry - Anti-NLRP3 antibody [EPR23073-96] - BSA and Azide free (ab272702)

        Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (untreated)(-) and  RAW 264.7 (+LPS 10ug/ml 8h)(+) cells labelling NLRP3 with ab270449 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in RAW 264.7 after treatment with LPS. ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

        Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270449).

      • Immunoprecipitation - Anti-NLRP3 antibody [EPR23073-96] - BSA and Azide free (ab272702)
        Immunoprecipitation - Anti-NLRP3 antibody [EPR23073-96] - BSA and Azide free (ab272702)

        NLRP3 was immunoprecipitated from 0.35 mg RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) (treated with 10 ug/ml Lipopolysaccharides (LPS) for 8 hours) whole cell lysate with ab270449 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab270449 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

        Lane 1: RAW 264.7 treated with 10 ug/ml Lipopolysaccharides (LPS) for 8 hours, whole cell lysate 10 ug

        Lane 2: ab270449 IP in RAW 264.7 treated with 10 ug/ml Lipopolysaccharides(LPS) for 8 hours, whole cell lysate

        Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab270449 in RAW 264.7 treated with 10 ug/ml Lipopolysaccharides(LPS) for 8 hours, whole cell lysate

        Blocking and dilution buffer and concentration: 5% NFDM/TBST.

        Exposure time: 24 seconds.

        The band around 75kDa corresponds to the short form of NLRP3.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270449).

      • Western blot - Anti-NLRP3 antibody [EPR23073-96] - BSA and Azide free (ab272702)
        Western blot - Anti-NLRP3 antibody [EPR23073-96] - BSA and Azide free (ab272702)
        All lanes : Anti-NLRP3 antibody [EPR23073-96] (ab270449) at 1/1000 dilution

        Lane 1 : Untreated RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
        Lane 2 : RAW 264.7 treated with /ml Lipopolysaccharides(LPS) for 8 hours whole cell lysate
        Lane 3 : J774A.1 (mouse reticulum cell sarcoma monocyte macrophage) whole cell lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

        Predicted band size: 118 kDa
        Observed band size: 118 kDa



        Blocking and diluting buffer and concentration: 5% NFDM/TBST.

        The molecular weight/expression profile observed is consistent with what has been described in the literature (PMID:26939933, 30315268 ).

        The band around 75kDa corresponds to the short form of NLRP3.

        Exposure time: 54 seconds. 

        This data was develoed using the same antibody clone in a different buffer formulation containing PBS, glycerol, BSA and sodium azide (ab270449).

      • Anti-NLRP3 antibody [EPR23073-96] - BSA and Azide free (ab272702)
        Anti-NLRP3 antibody [EPR23073-96] - BSA and Azide free (ab272702)

      プロトコール

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      参考文献 (0)

      ab272702 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab272702 は論文での使用が確認できていません。

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