Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (brain)
Permeabilization
Yes - triton
Specification
brain
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 30°C
Fixative
Paraformaldehyde
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti goat 488
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
I don't think there was a positive signal in the staining.
Abcam response
We would like to thank the reviewer for submitting this AbReview. As the product had not yet been tested in this species/application we were unsure about the result. We appreciate the time and effort that the reviewer has put in to testing this product.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jan 23 2020