Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human MDM)
Specification
human MDM
Fixative
Paraformaldehyde
Permeabilization
Yes - TRITON-X (0.1 %)
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/200
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS in 1 % BSA
Secondary antibody
Name
Non-Abcam antibody was used: donkey anti goat polyclonal
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy5 ®
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy5 ®
Dilution
1/400
Additional data
Additional Notes
nucleus stained as blue
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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投稿 Feb 06 2013