Application
Flow Cytometry
Sample
Human Cell (White Blood Cells (Mixed Population))
Specification
White Blood Cells (Mixed Population)
Preparation
Cell harvesting/tissue preparation method: WBC were isolated spinning Blood on Ficoll Gradient after removal of RBC's
Sample buffer: PBS
Sample buffer: PBS
Fixation
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X100 in 2% BSA for 15min
Gating Strategy
Monocytes
Other product details
Dilution
1/100
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% BSA in PBS
Secondary antibody
Dilution
1/250
Name
Non-Abcam antibody was used: Alexa Flour488 Donkey Anti Goat IgG (H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Mahesh Shivananjappa
Verified customer
投稿 Feb 15 2011