Anti-NLRP3 抗体 (ab4207)
Key features and details
- Goat polyclonal to NLRP3
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-NLRP3 antibody
NLRP3 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to NLRP3 -
由来種
Goat -
アプリケーション
適用あり: Flow Cyt, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat, Cow
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免疫原
Synthetic peptide corresponding to Human NLRP3 aa 1024-1036 (C terminal).
Sequence:C-EKPELTVVFEPSW
Database link: Q96P20-1 -
ポジティブ･コントロール
- ICC: U937 cells. Flow Cytometry: U937 cells.
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特記事項
No signal has been obtained in Western blot but low background has observed in Daudi, A431, Jurkat, U937 and MOLT-4 lysates at up to 1µg/ml. We would appreciate any feedback from people in the field.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab4207 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt | Use a concentration of 10 µg/ml. ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. -
組織特異性
Expressed in blood leukocytes. Strongly expressed in polymorphonuclear cells and osteoblasts. Undetectable or expressed at a lower magnitude in B- and T-lymphoblasts, respectively. High level of expression detected in chondrocytes. Detected in non-keratinizing epithelia of oropharynx, esophagus and ectocervix and in the urothelial layer of the bladder. -
関連疾患
Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1) [MIM:120100]; also known as familial cold urticaria. FCAS are rare autosomal dominant systemic inflammatory diseases characterized by episodes of rash, arthralgia, fever and conjunctivitis after generalized exposure to cold.
Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS) [MIM:191900]; also known as urticaria-deafness-amyloidosis syndrome. MWS is a hereditary periodic fever syndrome characterized by fever, chronic recurrent urticaria, arthralgias, progressive sensorineural deafness, and reactive renal amyloidosis. The disease may be severe if generalized amyloidosis occurs.
Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA) [MIM:607115]; also known as neonatal onset multisystem inflammatory disease (NOMID). CINCA is a rare congenital inflammatory disorder characterized by a triad of neonatal onset of cutaneous symptoms, chronic meningitis and joint manifestations with recurrent fever and inflammation. -
配列類似性
Belongs to the NLRP family.
Contains 1 DAPIN domain.
Contains 9 LRR (leucine-rich) repeats.
Contains 1 NACHT domain. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 114548 Human
- Entrez Gene: 287362 Rat
- Omim: 606416 Human
- SwissProt: Q96P20 Human
- Unigene: 159483 Human
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別名
- AGTAVPRL antibody
- AII/AVP antibody
- Angiotensin/vasopressin receptor AII/AVP like antibody
see all
画像
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Immunocytochemistry - Anti-NLRP3 antibody (ab4207)
Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Incubated with the primary antibody (ab4207) for 1hr at 10 µg/ml, followed by Alexa Fluor® 488 secondary antibody at 2 µg/ml. Shows cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/ml) followed by Alexa Fluor® 488 secondary antibody (2µg/ml).
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Flow Cytometry - Anti-NLRP3 antibody (ab4207)Flow cytometric analysis of paraformaldehyde fixed U937 cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab4207 was 1hr (10ug/ml) followed by Alexa Fluor® 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.
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Flow Cytometry - Anti-NLRP3 antibody (ab4207)This image is courtesy of an Abreview submitted by Mahesh Shivananjappa.ab4207 staining NLRP3 in the Human White Blood Cells (Mixed Population) by Flow Cytometry. WBC were isolated spinning Blood on Ficoll Gradient after removal of RBC's and permeabilized with 0.1% Triton-X100 in 2% BSA for 15 minutes. The sample was incubated with the primary antibody (1/100 in PBS + 2% BSA in PBS) for 16 hours at 4°C. An Alexa Flour® 488 Donkey Anti Goat IgG (H+L) (1/250) was used as the secondary antibody.
Gating Strategy: Monocytes -
Immunocytochemistry/ Immunofluorescence - Anti-NLRP3 antibody (ab4207)This image is courtesy of an anonymous AbreviewImmunofluorescence analysis of Human MDM cells, staining CIAS1 / NALP3 with ab4207.
Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% goat serum for 1 hour at room temp. Samples were incubated with primary antibody (1/200 in 1% BSA in PBS) for 12 hours at 4°C. A Cy5® conjugated donkey anti-goat polyclonal IgG (1/400) was used as the secondary antibody.
プロトコール
参考文献 (63)
ab4207 は 63 報の論文で使用されています。
- Garré JM et al. P2X7 receptor inhibition ameliorates dendritic spine pathology and social behavioral deficits in Rett syndrome mice. Nat Commun 11:1784 (2020). PubMed: 32286307
- Yan S et al. Pharmacological Inhibition of HDAC6 Attenuates NLRP3 Inflammatory Response and Protects Dopaminergic Neurons in Experimental Models of Parkinson's Disease. Front Aging Neurosci 12:78 (2020). PubMed: 32296327
- Chen F et al. Melatonin alleviates intervertebral disc degeneration by disrupting the IL-1ß/NF-?B-NLRP3 inflammasome positive feedback loop. Bone Res 8:10 (2020). PubMed: 32133213
- Josefs T et al. Neutrophil extracellular traps promote macrophage inflammation and impair atherosclerosis resolution in diabetic mice. JCI Insight 5:N/A (2020). PubMed: 32191637
- Zhao Y et al. Cortistatin protects against intervertebral disc degeneration through targeting mitochondrial ROS-dependent NLRP3 inflammasome activation. Theranostics 10:7015-7033 (2020). PubMed: 32550919
レビューと Q&A
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