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  1. Link

    nlrp3-antibody-ab4207.pdf

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Cell Biology Apoptosis Intracellular Associated Proteins
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Anti-NLRP3 抗体 (ab4207)

  • Datasheet
  • SDS
Reviews (8)Q&A (1)References (88)

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Abpromise

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Western blot - Anti-NLRP3 antibody (ab4207)
  • Flow Cytometry (Intracellular) - Anti-NLRP3 antibody (ab4207)
  • Flow Cytometry (Intracellular) - Anti-NLRP3 antibody (ab4207)

Key features and details

  • Goat polyclonal to NLRP3
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-NLRP3 antibody [EPR23073-96] (ab270449)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-NLRP3 antibody
    NLRP3 一次抗体 製品一覧
  • 製品の詳細

    Goat polyclonal to NLRP3
  • 由来種

    Goat
  • アプリケーション

    適用あり: Flow Cyt (Intra), WBmore details
    適用なし: ICC/IF
  • 種交差性

    交差種: Human
    交差が予測される動物種: Rat, Cow
  • 免疫原

    Synthetic peptide corresponding to Human NLRP3 aa 1024-1036 (C terminal).
    Sequence:

    C-EKPELTVVFEPSW


    Database link: Q96P20-1
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ・コントロール

    • Flow Cyt (intra): U937 cells. WB: THP-1 cells
  • 特記事項

    No signal has been obtained in Western blot but low background has observed in Daudi, A431, Jurkat, U937 and MOLT-4 lysates at up to 1µg/ml. We would appreciate any feedback from people in the field.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 特記事項(精製)

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Associated Proteins
    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Cancer
    • Growth factors
    • TNF
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Neuroscience
    • Processes

関連製品

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Recombinant Protein

    • Recombinant Human NLRP3 protein (ab165022)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab4207の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
Use a concentration of 10 µg/ml.

ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

WB (2)
1/1000. Predicted molecular weight: 118 kDa.
特記事項
Flow Cyt (Intra)
Use a concentration of 10 µg/ml.

ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000. Predicted molecular weight: 118 kDa.
追加情報
Is unsuitable for ICC/IF.

ターゲット情報

  • 機能

    May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18.
  • 組織特異性

    Expressed in blood leukocytes. Strongly expressed in polymorphonuclear cells and osteoblasts. Undetectable or expressed at a lower magnitude in B- and T-lymphoblasts, respectively. High level of expression detected in chondrocytes. Detected in non-keratinizing epithelia of oropharynx, esophagus and ectocervix and in the urothelial layer of the bladder.
  • 関連疾患

    Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1) [MIM:120100]; also known as familial cold urticaria. FCAS are rare autosomal dominant systemic inflammatory diseases characterized by episodes of rash, arthralgia, fever and conjunctivitis after generalized exposure to cold.
    Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS) [MIM:191900]; also known as urticaria-deafness-amyloidosis syndrome. MWS is a hereditary periodic fever syndrome characterized by fever, chronic recurrent urticaria, arthralgias, progressive sensorineural deafness, and reactive renal amyloidosis. The disease may be severe if generalized amyloidosis occurs.
    Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA) [MIM:607115]; also known as neonatal onset multisystem inflammatory disease (NOMID). CINCA is a rare congenital inflammatory disorder characterized by a triad of neonatal onset of cutaneous symptoms, chronic meningitis and joint manifestations with recurrent fever and inflammation.
  • 配列類似性

    Belongs to the NLRP family.
    Contains 1 DAPIN domain.
    Contains 9 LRR (leucine-rich) repeats.
    Contains 1 NACHT domain.
  • 細胞内局在

    Cytoplasm.
  • Target information above from: UniProt accession Q96P20 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 114548 Human
    • Entrez Gene: 287362 Rat
    • Omim: 606416 Human
    • SwissProt: Q96P20 Human
    • Unigene: 159483 Human
    • 別名

      • AGTAVPRL antibody
      • AII/AVP antibody
      • Angiotensin/vasopressin receptor AII/AVP like antibody
      • Angiotensin/vasopressin receptor AII/AVP-like antibody
      • C1orf7 antibody
      • Caterpiller protein 1.1 antibody
      • CIAS 1 antibody
      • CIAS1 antibody
      • CLR1.1 antibody
      • Cold autoinflammatory syndrome 1 antibody
      • Cold autoinflammatory syndrome 1 protein antibody
      • Cryopyrin antibody
      • Familial cold autoinflammatory syndrome antibody
      • FCAS antibody
      • FCU antibody
      • LRR and PYD domains-containing protein 3 antibody
      • Muckle-Wells syndrome antibody
      • MWS antibody
      • NACHT antibody
      • NACHT LRR and PYD containing protein 3 antibody
      • NALP 3 antibody
      • NALP3 antibody
      • NALP3_HUMAN antibody
      • NLR family pyrin domain containing 3 antibody
      • NLRP3 antibody
      • PYPAF 1 antibody
      • PYPAF1 antibody
      • PYRIN containing APAF1 like protein 1 antibody
      • PYRIN-containing APAF1-like protein 1 antibody
      see all

    画像

    • Western blot - Anti-NLRP3 antibody (ab4207)
      Western blot - Anti-NLRP3 antibody (ab4207)
      All lanes : Anti-NLRP3 antibody (ab4207) at 1/1000 dilution

      Lane 1 : Wild-type THP-1 cell lysate
      Lane 2 : NLRP3 knockout THP-1 cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 118 kDa
      Observed band size: 118 kDa



      False colour image of Western blot: Anti-NLRP3 antibody staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab4207 was shown to bind specifically to NLRP3. A band was observed at 118 kDa in wild-type THP-1 cell lysates with no signal observed at this size in NLRP3 knockout cell line ab280063 (knockout cell lysate ab280122). To generate this image, wild-type and NLRP3 knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Donkey anti-Goat IgG H&L (IRDye® 800CW) preabsorbed (ab216775) and Donkey anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216778) at 1/20000 dilution.

    • Flow Cytometry (Intracellular) - Anti-NLRP3 antibody (ab4207)
      Flow Cytometry (Intracellular) - Anti-NLRP3 antibody (ab4207)

      Flow cytometric analysis of paraformaldehyde fixed U937 cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab4207 was 1hr (10ug/ml) followed by Alexa Fluor® 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.

    • Flow Cytometry (Intracellular) - Anti-NLRP3 antibody (ab4207)
      Flow Cytometry (Intracellular) - Anti-NLRP3 antibody (ab4207)This image is courtesy of an Abreview submitted by Mahesh Shivananjappa.

      ab4207 staining NLRP3 in the Human White Blood Cells (Mixed Population) by Flow Cytometry. WBC were isolated spinning Blood on Ficoll Gradient after removal of RBC's and permeabilized with 0.1% Triton-X100 in 2% BSA for 15 minutes. The sample was incubated with the primary antibody (1/100 in PBS + 2% BSA in PBS) for 16 hours at 4°C. An Alexa Flour® 488 Donkey Anti Goat IgG (H+L) (1/250) was used as the secondary antibody.
      Gating Strategy: Monocytes

      See Abreview

    プロトコール

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (88)

    ab4207 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab4207 は 88 報の論文で使用されています。

    • Qin Y  et al. Impaired autophagy in microglia aggravates dopaminergic neurodegeneration by regulating NLRP3 inflammasome activation in experimental models of Parkinson's disease. Brain Behav Immun 91:324-338 (2021). PubMed: 33039664
    • Chen Y  et al. NLRP3 regulates alveolar bone loss in ligature-induced periodontitis by promoting osteoclastic differentiation. Cell Prolif 54:e12973 (2021). PubMed: 33382502
    • Cheng Y  et al. iTRAQ-Based Quantitative Proteomics Indicated Nrf2/OPTN-Mediated Mitophagy Inhibits NLRP3 Inflammasome Activation after Intracerebral Hemorrhage. Oxid Med Cell Longev 2021:6630281 (2021). PubMed: 33628368
    • Liu X  et al. Bone marrow mesenchymal stem cell-derived exosomes attenuate cerebral ischemia-reperfusion injury-induced neuroinflammation and pyroptosis by modulating microglia M1/M2 phenotypes. Exp Neurol 341:113700 (2021). PubMed: 33741350
    • Zheng S  et al. microRNA-129 overexpression in endothelial cell-derived extracellular vesicle influences inflammatory response caused by myocardial ischemia/reperfusion injury. Cell Biol Int 45:1743-1756 (2021). PubMed: 33851774
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-9 of 9 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-NLRP3 antibody

    Average
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human Primary Fibroblast)
    Permeabilization
    Yes - 0.1% Triton 1h
    Specification
    Human Primary Fibroblast
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 20 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-NLRP3 antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (brain)
    Permeabilization
    Yes - triton
    Specification
    brain
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 30°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 23 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-NLRP3 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Brain)
    Permeabilization
    Yes - 5% Triton-X in 0.05M Phosphate Buffered Saline
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 28 2018

    Western blot abreview for Anti-NLRP3 antibody

    Below Average
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (macrophage)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Treatment
    0.1 ug/ml LPS 15 min
    Specification
    macrophage
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 12 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CIAS1 / NALP3 antibody

    Good
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (human MDM)
    Specification
    human MDM
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - TRITON-X (0.1 %)
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 06 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CIAS1 / NALP3 antibody

    Good
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (RAW 264.7)
    Specification
    RAW 264.7
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton-X100 in 2% BSA for 15min
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
    Read More

    Dr. Mahesh Shivananjappa

    Verified customer

    投稿 May 24 2012

    Western blot abreview for Anti-CIAS1 / NALP3 antibody

    Poor
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Corpus Cavernosum)
    Loading amount
    20 µg
    Specification
    Corpus Cavernosum
    Gel Running Conditions
    Reduced Denaturing (12)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Jason Davis

    Verified customer

    投稿 Jan 13 2012

    Flow Cytometry abreview for Anti-CIAS1 / NALP3 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (White Blood Cells (Mixed Population))
    Specification
    White Blood Cells (Mixed Population)
    Preparation
    Cell harvesting/tissue preparation method: WBC were isolated spinning Blood on Ficoll Gradient after removal of RBC's
    Sample buffer: PBS
    Fixation
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton-X100 in 2% BSA for 15min
    Gating Strategy
    Monocytes
    Read More

    Dr. Mahesh Shivananjappa

    Verified customer

    投稿 Feb 15 2011

    Question

    I would like to test ab4207 for use on canine samples.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 29 2012

    Answer

    DISCOUNT CODE: *** Expiration date: February 29th, 2013 Value: *** I am very pleased to hear you would like to accept our offer and test ab4207 in canine. This code will give you *** off your next order before the expiration date. To redeem this offer, please submit an Abreview for canine and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

    Read More

    Abcam Scientific Support

    Answered on Oct 29 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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