Anti-NLK 抗体 (ab97642)
Key features and details
- Rabbit polyclonal to NLK
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-NLK antibody
NLK 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NLK -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human NLK aa 284-500.
Database link: Q9UBE8 -
ポジティブ･コントロール
- WB: HeLa, HEK-293T cell extract. Mouse brain lysate. ICC/IF: HeLa cells. IHC-P: Mouse ovary tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97642の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/100 - 1/1000.
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| WB | (3) |
1/500 - 1/3000. Predicted molecular weight: 58 kDa.
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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IHC-P
1/100 - 1/1000. |
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WB
1/500 - 1/3000. Predicted molecular weight: 58 kDa. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Role in cell fate determination, required for differentiation of bone marrow stromal cells. Acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt/beta-catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. May suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP (By similarity). Involved in TGFbeta-mediated mesoderm induction, acting downstream of MAP3K7/TAK1 to phosphorylate STAT3. -
配列類似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Dually phosphorylated on Thr-303 and Tyr-305, which activates the enzyme. -
細胞内局在
Nucleus. Cytoplasm. Predominantly nuclear. A smaller fraction is cytoplasmic. - Information by UniProt
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参照データベース
- Entrez Gene: 51701 Human
- Entrez Gene: 18099 Mouse
- Omim: 609476 Human
- SwissProt: Q9UBE8 Human
- SwissProt: O54949 Mouse
- Unigene: 208759 Human
- Unigene: 9001 Mouse
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別名
- DKFZp761G1211 antibody
- FLJ21033 antibody
- LAK1 antibody
see all
画像
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Western blot - Anti-NLK antibody (ab97642)Anti-NLK antibody (ab97642) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 58 kDa
7.5% SDS-PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-NLK antibody (ab97642)Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab97642 at 1/100 dilution. Lower image is co-stained with Hoechst 33342. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLK antibody (ab97642)Paraffin embedded mouse ovary tissue stained for NLK using ab97642 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-NLK antibody (ab97642)All lanes : Anti-NLK antibody (ab97642) at 1/500 dilution
Lane 1 : Non-transfected HEK-293T whole cell extract
Lane 2 : Transfected HEK-293T whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 58 kDa10% SDS-PAGE
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Western blot - Anti-NLK antibody (ab97642)Anti-NLK antibody (ab97642) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 58 kDa7.5% SDS PAGE
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab97642 は 5 報の論文で使用されています。
- Wilkes MC et al. The active component of ginseng, ginsenoside Rb1, improves erythropoiesis in models of Diamond-Blackfan anemia by targeting Nemo-like kinase. J Biol Chem 297:100988 (2021). PubMed: 34298020
- Li CZ et al. RIP1 regulates TNF-a-mediated lymphangiogenesis and lymphatic metastasis in gallbladder cancer by modulating the NF-?B-VEGF-C pathway. Onco Targets Ther 11:2875-2890 (2018). PubMed: 29844685
- Yan X et al. Impact of miR-208 and its Target Gene Nemo-Like Kinase on the Protective Effect of Ginsenoside Rb1 in Hypoxia/Ischemia Injuried Cardiomyocytes. Cell Physiol Biochem 39:1187-95 (2016). PubMed: 27577116
- Huang T et al. A regulatory circuit of miR-125b/miR-20b and Wnt signalling controls glioblastoma phenotypes through FZD6-modulated pathways. Nat Commun 7:12885 (2016). PubMed: 27698350
- Shaw-Hallgren G et al. Association of nuclear-localized Nemo-like kinase with heat-shock protein 27 inhibits apoptosis in human breast cancer cells. PLoS One 9:e96506 (2014). PubMed: 24816797