Anti-NKG2D 抗体 (ab203353)
Key features and details
- Rabbit polyclonal to NKG2D
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-NKG2D antibody
NKG2D 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NKG2D -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide within Rat NKG2D aa 100-200 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O70215 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab203353の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
1/20 - 1/100.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
(or 1/50 -1/200 using a fluorescent secondary antibody). |
特記事項 |
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Flow Cyt
1/20 - 1/100. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. (or 1/50 -1/200 using a fluorescent secondary antibody). |
ターゲット情報
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機能
Receptor for MICA, MICB, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4. Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. Involved in the immune surveillance exerted by T- and B-lymphocytes. -
組織特異性
Natural killer cells. Expressed on essentially all CD56+CD3- NK cells from freshly isolated PBMC. Also detected in gamma-delta cells and CD8+ alpha-beta T-cells. Expressed in interferon-producing killer dendritic cells (IKDCs). -
配列類似性
Contains 1 C-type lectin domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 100528032 Human
- Entrez Gene: 22914 Human
- Entrez Gene: 27007 Mouse
- Omim: 611817 Human
- SwissProt: P26718 Human
- SwissProt: O54709 Mouse
- Unigene: 387787 Human
- Unigene: 8217 Mouse
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別名
- CD314 antibody
- CD314 antigen antibody
- D12S2489E antibody
see all
画像
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Immunohistochemical analysis of formalin-fixed, paraffin embedded Human nasopharyngeal carcinoma tissue labeling NKG2D with ab203353 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Mouse thymus cells probed with ab203353 (green) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)
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ab203353 staining NKG2D in mouse lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 20% serum for 20 minutes at 37°C; antigen retrieval was by heat mediation in a citrate buffer for 15 minutes. Samples were incubated with primary antibody (1/400 in blocking buffer) overnight at 4°C, followed by a conjugated secondary and DAB staining.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab203353 は 9 報の論文で使用されています。
- Wang H et al. CDX2 enhances natural killer cell-mediated immunotherapy against head and neck squamous cell carcinoma through up-regulating CXCL14. J Cell Mol Med 25:4596-4607 (2021). PubMed: 33733587
- Lee GH et al. Clinical Impact of Natural Killer Group 2D Receptor Expression and That of Its Ligand in Ovarian Carcinomas: A Retrospective Study. Yonsei Med J 62:288-297 (2021). PubMed: 33779082
- Čepcová D et al. The protective effect of 1-methyltryptophan isomers in renal ischemia-reperfusion injury is not exclusively dependent on indolamine 2,3-dioxygenase inhibition. Biomed Pharmacother 135:111180 (2021). PubMed: 33433354
- Spielmann J et al. Effects of obesity on NK cells in a mouse model of postmenopausal breast cancer. Sci Rep 10:20606 (2020). PubMed: 33244094
- Kravtsova-Ivantsiv Y et al. Excess of the NF-?B p50 subunit generated by the ubiquitin ligase KPC1 suppresses tumors via PD-L1- and chemokines-mediated mechanisms. Proc Natl Acad Sci U S A 117:29823-29831 (2020). PubMed: 33168738
- Mackiewicz Z et al. Sjögren's Syndrome: Concerted Triggering of Sicca Conditions. J Immunol Res 2019:2075803 (2019). PubMed: 30723748
- Zhang K et al. Splenic NKG2D confers resilience versus susceptibility in mice after chronic social defeat stress: beneficial effects of (R)-ketamine. Eur Arch Psychiatry Clin Neurosci N/A:N/A (2019). PubMed: 31875248
- Mo Z et al. Ultrasound-guided radiofrequency ablation enhances natural killer-mediated antitumor immunity against liver cancer. Oncol Lett 15:7014-7020 (2018). PubMed: 29725428
- Yin X et al. Role of NKG2D in cytokine-induced killer cells against lung cancer. Oncol Lett 13:3139-3143 (2017). PubMed: 28529563