Nitric Oxide Assay Kit (ab272517)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate
- Sample type: Cell culture supernatant, Cell Lysate, Plasma, Saliva, Serum, Tissue Extracts, Urine, Whole Blood
製品の概要
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製品名
Nitric Oxide Assay Kit
Nitric oxide キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Saliva, Urine, Serum, Plasma, Whole Blood, Tissue Extracts, Cell Lysate -
アッセイタイプ
Quantitative -
検出範囲
0.6 µM - 200 µM -
製品の概要
Nitric Oxide Assay Kit (ab272517) is a simple, direct and automation-ready procedure for measuring NO. Since NO is oxidized to nitrite and nitrate, it is common practice to quantitate total NO2 - /NO3 - as a measure for NO level. This Nitric Oxide Assay Kit is designed to accurately measure NO production following reduction of nitrate to nitrite using an improved Griess method. The procedure is simple and the time required for sample pre-treatment and assay is reduced to as little as 30 minutes.
Sensitive and accurate: Detection range 0.6 - 200 µM in 96-well plate.
Rapid and reliable: Using an optimized VCl3 reagent, the time required for reduction of NO3 – to NO2 - is 10 min at 60°C.
Simple and high-throughput: The procedure involves mixing sample with three reagents, incubation for 10 minutes at 60°C and reading the optical density. Can be readily automated to measure thousands of samples per day.
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アプリケーション
適用あり: Functional Studiesmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests NaOH 1 x 1ml Nitrite Standard (1 mM) 1 x 1ml Reagent A 1 x 12ml Reagent B 1 x 500µl Reagent C 1 x 12ml ZnSO4 1 x 1ml -
研究分野
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関連性
Nitric oxide (NO) is a key vertebrate biological messenger, playing an important role in neurotransmission, vascular regulation, immune responses and apoptosis. NO , also known as "endothelium-derived relaxing factor" or "EDRF", is synthesized from L-arginine, oxygen and NADPH by various NO synthases. Most of the NO in the cell is oxidized to nitrite (NO2-) and nitrate (NO3-), and therefore the concentrations of these anions are generally as a quantitative measure of NO production.
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab272517の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Functional Studies |
Use at an assay dependent concentration.
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特記事項 |
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Functional Studies
Use at an assay dependent concentration. |
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データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab272517 は 5 報の論文で使用されています。
- Giannasi C et al. Human Osteochondral Explants as an Ex Vivo Model of Osteoarthritis for the Assessment of a Novel Class of Orthobiologics. Pharmaceutics 14:N/A (2022). PubMed: 35745803
- El-Mancy EM et al. α-Lipoic Acid Protects against Cyclosporine A-Induced Hepatic Toxicity in Rats: Effect on Oxidative Stress, Inflammation, and Apoptosis. Toxics 10:N/A (2022). PubMed: 36006121
- Liu X et al. The protective role of miR-132 targeting HMGA2 through the PI3K/AKT pathway in mice with Alzheimer's disease. Am J Transl Res 13:4632-4643 (2021). PubMed: 34150043
- Abdolmaleki A et al. New rat to mouse xenograft transplantation of endometrium as a model of human endometriosis. Animal Model Exp Med 4:268-277 (2021). PubMed: 34557653
- Ghoneim FM et al. Evaluation of Endothelial Dysfunction and Autophagy in Fibromyalgia-Related Vascular and Cerebral Cortical Changes and the Ameliorative Effect of Fisetin. Cells 11:N/A (2021). PubMed: 35011610