Key features and details
- Goat polyclonal to NIPP1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
NIPP1 一次抗体 製品一覧
製品の詳細Goat polyclonal to NIPP1
アプリケーション適用あり: IHC-P, WBmore details
種交差性交差種: Mouse, Human
交差が予測される動物種: Rat, Cow, Dog
- WB: HeLa, HepG2, Jurkat and NIH3T3 nuclear lysates. IHC-P: Human thymus tissue.
This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA.
保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA
Concentration information loading...
精製度Immunogen affinity purified
特記事項（精製）Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
一次抗体 備考This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA.
Our Abpromise guarantee covers the use of ab5300 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 2.5 - 3.8 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
1 hour primary incubation is recommended for this product.
機能Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation.
Isoform Gamma is a site-specific single-strand endoribonuclease that cleaves single strand RNA 3' to purines and pyrimidines in A+U-rich regions. It generates 5'-phosphate termini at the site of cleavage. This isoform does not inhibit PP-1. May be implicated in mRNA splicing.
組織特異性Ubiquitously expressed, with highest levels in heart and skeletal muscle, followed by brain, placenta, lung, liver and pancreas. Less abundant in kidney. The concentration and ratio between isoforms is cell-type dependent. Isoform Alpha (>90%) and isoform Beta were found in brain, heart and kidney. Isoform Gamma is mainly found in B-cells and T-lymphocytes, and has been found in 293 embryonic kidney cells.
配列類似性Contains 1 FHA domain.
ドメインHas a basic N- and C-terminal and an acidic central domain.
翻訳後修飾May be inactivated by phosphorylation on Ser-199 or Ser-204 (By similarity). Phosphorylated by Lyn in vitro on Tyr-264, and also on Tyr-335 in the presence of RNA.
細胞内局在Nucleus. Nucleus speckle. Primarily, but not exclusively, nuclear and Cytoplasm. Found mainly in the cytoplasm.
- Information by UniProt
- Activator of RNA decay antibody
- ARD 1 antibody
- ARD-1 antibody
All lanes : Anti-NIPP1 antibody (ab5300) at 1 µg/ml
Lane 1 : HeLa nuclear lysate in RIPA buffer
Lane 2 : HepG2 nuclear lysate in RIPA buffer
Lane 3 : Jurkat nuclear lysate in RIPA buffer
Lane 4 : NIH3T3 nuclear lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 40 kDa
Primary incubation for 1 hour. Detected using chemiluminescence.
ab5300 (3.8µg/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
ab5300 は 1 報の論文で使用されています。
- Nuytten M et al. The transcriptional repressor NIPP1 is an essential player in EZH2-mediated gene silencing. Oncogene 27:1449-60 (2008). WB . PubMed: 17724462