Application
Western blot
Sample
Human Purified protein (HT1080)
Gel Running Conditions
Reduced Denaturing (Tris-Acetate NuPage Gel 3-8%)
Loading amount
10000 cells
Specification
HT1080
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Diluent: 1.5% milk in PBST
Dilution
1/2000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: AntiRat HPRT
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
15 minute(s) and 0 second(s)
Bands
Specific: 315 kDa
Additional data
Additional Notes
Spectra Multicolor High Range Protein Marker in first lane and HT1080 Protein Extract in Second. Light image showing markers alongside HPRT exposure.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Leisha Nolen
Verified customer
投稿 Feb 03 2012