製品名Nigericin Na+ salt, K+ ionophore
生理活性の詳細K+ ionophore; exchanges K+ for H+ across most biological membranes. Stimulates mitochondrial ATPase activity, and exchanges H+ for K+>Rb+>Cs+>>Na+. Reported to inhibit Poliovirus and influenza virus replication.
体系名Nigericin sodium salt
保存方法Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
溶解性Soluble in ethanol to 100 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Our Abpromise guarantee covers the use of ab120494 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab56889 staining mitofusin 2 in MEF1 cells treated with nigericin Na+ salt (ab120494), by ICC/IF. Decrease in mitofusin 2 expression correlates with increased concentration of nigericin Na+ salt, as described in literature.
The cells were incubated at 37°C for 3h in media containing different concentrations of ab120494 (nigericin Na+ salt) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab56889 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
This product has been referenced in:
- Myskiw C et al. Nigericin is a potent inhibitor of the early stage of vaccinia virus replication. Antiviral Res 88:304-10 (2010). Read more (PubMed: 20951746) »
- Margolis LB et al. K+/H+-antiporter nigericin arrests DNA synthesis in Ehrlich ascites carcinoma cells. Proc Natl Acad Sci U S A 86:6626-9 (1989). Read more (PubMed: 2771947) »
- Daniele RP et al. A potassium ionophore (Nigericin) inhibits stimulation of human lymphocytes by mitogens. J Exp Med 147:571-81 (1978). Read more (PubMed: 146727) »