Anti-Nidogen 2/NID-2 抗体 (ab14513)
Key features and details
- Rabbit polyclonal to Nidogen 2/NID-2
- Suitable for: WB, ELISA, IHC-Fr, ICC/IF, Flow Cyt
- Reacts with: Mouse, Recombinant fragment
- Isotype: IgG
製品の概要
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製品名
Anti-Nidogen 2/NID-2 antibody
Nidogen 2/NID-2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Nidogen 2/NID-2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ELISA, IHC-Fr, ICC/IF, Flow Cytmore details -
種交差性
交差種: Mouse, Recombinant fragment -
免疫原
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab14513の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 153 kDa.
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| ELISA |
Use at an assay dependent concentration.
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| IHC-Fr |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration. PubMed: 23409002
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| Flow Cyt |
Use at an assay dependent concentration. PubMed: 23211522
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 153 kDa. |
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ELISA
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. PubMed: 23409002 |
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Flow Cyt
Use at an assay dependent concentration. PubMed: 23211522 ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Cell adhesion glycoprotein which is widely distributed in basement membranes. Binds to collagens I and IV, to perlecan and to laminin 1. Does not bind fibulins. It probably has a role in cell-extracellular matrix interactions. -
組織特異性
Heart, placenta and bone. Less in pancreas, kidney and skeletal muscle. -
配列類似性
Contains 5 EGF-like domains.
Contains 5 LDL-receptor class B repeats.
Contains 1 NIDO domain.
Contains 1 nidogen G2 beta-barrel domain.
Contains 2 thyroglobulin type-1 domains. -
翻訳後修飾
Highly N- and O-glycosylated. -
細胞内局在
Secreted > extracellular space > extracellular matrix > basement membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 18074 Mouse
- SwissProt: O88322 Mouse
- Unigene: 20348 Mouse
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別名
- NID 2 antibody
- NID-2 antibody
- Nid2 antibody
see all
画像
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Western blot - Anti-Nidogen 2/NID-2 antibody (ab14513)All lanes : Anti-Nidogen 2/NID-2 antibody (ab14513) at 1/400 dilution
Lane 1 : Recombinant Nidogen 2 at 0.005 µg
Lane 2 : Recombinant Nidogen 1
Lane 3 : Protein extract of mouse femoral heads. The femoral head extract was prepared by incubating 3 mouse femoral heads (total weight 20 mg) in 200 µl buffer (0.05 M Tris; 0.15 M NaCl; 10 mM EDTA; 6 M Urea, pH 7.4) overnight. at 20 µl
Performed under reducing conditions.
Predicted band size: 153 kDa
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (9)
ab14513 は 9 報の論文で使用されています。
- Wells HRR et al. GWAS Identifies 44 Independent Associated Genomic Loci for Self-Reported Adult Hearing Difficulty in UK Biobank. Am J Hum Genet 105:788-802 (2019). PubMed: 31564434
- Nordquist E et al. Postnatal and Adult Aortic Heart Valves Have Distinctive Transcriptional Profiles Associated With Valve Tissue Growth and Maintenance Respectively. Front Cardiovasc Med 5:30 (2018). IHC-Fr ; Mouse . PubMed: 29740591
- Murphy PA et al. Tumor angiogenesis in the absence of fibronectin or its cognate integrin receptors. PLoS One 10:e0120872 (2015). PubMed: 25807551
- Torricelli AA et al. Epithelial basement membrane proteins perlecan and nidogen-2 are up-regulated in stromal cells after epithelial injury in human corneas. Exp Eye Res 134:33-8 (2015). PubMed: 25797478
- Bercsenyi K et al. Tetanus toxin entry. Nidogens are therapeutic targets for the prevention of tetanus. Science 346:1118-23 (2014). PubMed: 25430769
- Hummitzsch K et al. A new model of development of the mammalian ovary and follicles. PLoS One 8:e55578 (2013). ICC/IF ; Cow . PubMed: 23409002
- Behrens DT et al. The epidermal basement membrane is a composite of separate laminin- or collagen IV-containing networks connected by aggregated perlecan, but not by nidogens. J Biol Chem 287:18700-9 (2012). PubMed: 22493504
- Irving-Rodgers HF et al. PANCREATIC ISLET BASEMENT MEMBRANE LOSS AND REMODELING AFTER MOUSE ISLET ISOLATION AND TRANSPLANTATION: IMPACT FOR ALLOGRAFT REJECTION. Cell Transplant N/A:N/A (2012). IHC-Fr, Flow Cyt ; Mouse . PubMed: 23211522
- Lee HK et al. Nidogen is a prosurvival and promigratory factor for adult Schwann cells. J Neurochem 102:686-98 (2007). PubMed: 17437540
