Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 抗体 (ab41172)
Key features and details
- Rabbit polyclonal to Nicotinic Acetylcholine Receptor alpha 4/CHRNA4
- Suitable for: ELISA, ICC/IF, IHC-P, WB, IHC-Fr
- Reacts with: Mouse, Rat, Chicken, Human, Chimpanzee, Pufferfish, Zebrafish, Snail
- Isotype: IgG
製品の概要
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製品名
Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody
Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 -
由来種
Rabbit -
特異性
Antiserum Specificity Polypeptide % Cross Reactivity Nicotinic a4 receptor (620-627) 100 Nicotinic a4 receptor ~80 Nicotinic a3 receptor 0 Nicotinic a5 receptor 0 Nicotinic a7 receptor 0 Nicotinic ß2 receptor 0 Nicotinic ß3 receptor 0 Nicotinic ß4 receptor 0 -
アプリケーション
適用あり: ELISA, ICC/IF, IHC-P, WB, IHC-Frmore details -
種交差性
交差種: Mouse, Rat, Chicken, Human, Chimpanzee, Pufferfish, Zebrafish, Snail -
免疫原
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Constituents: PBS, 1% BSA -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab41172の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P |
1/250.
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WB |
1/800. Predicted molecular weight: 70 kDa. With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.
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IHC-Fr | (1) |
Use at an assay dependent concentration.
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特記事項 |
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ELISA
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
1/250. |
WB
1/800. Predicted molecular weight: 70 kDa. With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk. |
IHC-Fr
Use at an assay dependent concentration. |
ターゲット情報
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機能
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. -
関連疾患
Defects in CHRNA4 are the cause of nocturnal frontal lobe epilepsy type 1 (ENFL1) [MIM:600513]; also symbolized ADNFLE. ENFL1 is an autosomal dominant epilepsy characterized by nocturnal seizures with hyperkinetic automatisms and poorly organized stereotyped movements. -
配列類似性
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-4/CHRNA4 sub-subfamily. -
細胞内局在
Cell junction > synapse > postsynaptic cell membrane. Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 395606 Chicken
- Entrez Gene: 1137 Human
- Entrez Gene: 11438 Mouse
- Entrez Gene: 25590 Rat
- Omim: 118504 Human
- SwissProt: P09482 Chicken
- SwissProt: P43681 Human
- SwissProt: O70174 Mouse
see all -
別名
- A4 nicotinic receptor antibody
- Acetylcholine receptor alpha 4 neural antibody
- Acetylcholine receptor neuronal nicotinic alpha 4 subunit antibody
see all
画像
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All lanes : Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody (ab41172) at 1/800 dilution
Lane 1 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 70 kDa -
ab41172 (1/250 dilution) staining Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 in human cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclear and membrane compartment within the glia region of cells
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (5)
ab41172 は 5 報の論文で使用されています。
- Wang Q et al. Nicotine Activating α4β2 Nicotinic Acetylcholine Receptors to Suppress Neuroinflammation via JAK2-STAT3 Signaling Pathway in Ischemic Rats and Inflammatory Cells. Mol Neurobiol 59:3280-3293 (2022). PubMed: 35297011
- Seoane-Collazo P et al. Central nicotine induces browning through hypothalamic ? opioid receptor. Nat Commun 10:4037 (2019). PubMed: 31492869
- Montaño-Velázquez BB et al. Influence of Tobacco Smoke Exposure on the Protein Expression of a7 and a4 Nicotinic Acetylcholine Receptors in Squamous Cell Carcinoma Tumors of the Upper Aerodigestive Tract (Out of the Larynx). Subst Abuse 12:1178221818801316 (2018). PubMed: 31068752
- Chen N et al. An acetylcholine-activated microcircuit drives temporal dynamics of cortical activity. Nat Neurosci 18:892-902 (2015). IHC . PubMed: 25915477
- Pubill D et al. 3,4-Methylenedioxy-methamphetamine induces in vivo regional up-regulation of central nicotinic receptors in rats and potentiates the regulatory effects of nicotine on these receptors. Neurotoxicology 35:41-9 (2013). PubMed: 23261423