Key features and details
- Rabbit polyclonal to Nicastrin
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Nicastrin 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to Nicastrin
アプリケーション適用あり: WBmore details
種交差性交差種: Mouse, Rat, Human
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファーPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
Concentration information loading...
精製度Immunogen affinity purified
一次抗体 備考Amyloid beta peptide is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimer’s disease. This peptide is generated from the beta-amyloid precursor protein (beta APP) in a two-step process. The first step involves cleavage of the extracellular, amino-terminal domain of beta APP. Protein cleavage is performed by an aspartyl protease termed beta-secretase (BACE). This enzyme is synthesized as a propeptide that must be modified to the mature and active form by the prohormone convertase, furin. Beta APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta APP and a membrane-bound remnant. This remnant is then processed by another protease termed gamma-secretase. Gamma-secretase cleaves an intra-membrane site in the carboxyl-terminal domain of beta APP, thus generating the amyloid beta peptide. Gamma-secretase is believed to be a multi-subunit complex containing presenilin-1 and 2 as central components. Found associated with the presenilins is the transmembrane glycoprotein nicastrin. Nicastrin has been found to bind to the carboxyl-terminus of beta APP and helps to modulate the production of the amyloid beta peptide. Also found in the neurofibrillary lesions associated with Alzheimer’s disease is a protein termed Tau. Tau is a neuronal microtubule-associated protein found predominantly on axons. Tau functions to promote tubulin polymerization and stabilize microtubules. Tau, in its hyperphosphorylated form, is the major constituent of paired helical filaments (PHF), which are the building block of neurofibrillary lesions found in brain tissue of Alzheimer’s diseased patients.
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab3444 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 78 kDa).Can be blocked with Nicastrin peptide (ab4977).|
機能Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor required for the assembly of the gamma-secretase complex.
配列類似性Belongs to the nicastrin family.
細胞内局在Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- Anterior pharynx defective 2 antibody
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ab3444 は 3 報の論文で使用されています。
- Pintchovski SA et al. Evidence that enzyme processivity mediates differential Aß production by PS1 and PS2. Curr Alzheimer Res 10:4-10 (2013). WB . PubMed: 22950863
- Steuble M et al. Calsyntenin-1 shelters APP from proteolytic processing during anterograde axonal transport. Biol Open 1:761-74 (2012). PubMed: 23213470
- Massey LK et al. Ubiquilin regulates presenilin endoproteolysis and modulates gamma-secretase components, Pen-2 and nicastrin. Biochem J 391:513-25 (2005). PubMed: 15975090