製品名Nicardipine hydrochloride, L-type Ca2+ channel antagonist
製品の詳細L-type Ca2+ channel antagonist
生理活性の詳細Selective, L-type Ca2+ channel blocker (IC50 = 24.1 μM). Dihydropyridine derivative. Potent coronary and cerebral vasodilator.
体系名1,4-Dihydro-2,6-dimethyl-4-(3-nitrophenyl)methyl-2-[methyl(phenylmethyl)amino]-3,5-pyridinedicarboxylic acid ethyl ester hydrochloride
保存方法Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
溶解性Soluble in DMSO to 100 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information.
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Our Abpromise guarantee covers the use of ab120531 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
HepG2 cells were incubated at 37°C for 48h with vehicle control (0 µM) and different concentrations of nicardipine hydrochloride (ab120531) in DMSO. Increased expression of cytochrome P450 3A4 (ab124921) correlates with an increase in nicardipine hydrochloride concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab124921 at 1/10000 dilution and ab9484 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
This product has been referenced in:
- Yui H et al. Comparative effects of verapamil, nicardipine, and nitroglycerin on myocardial ischemia/reperfusion injury. Anesthesiol Res Pract 2011:521084 (2011). Read more (PubMed: 21403860) »
- Furukawa T et al. Selectivities of dihydropyridine derivatives in blocking Ca(2+) channel subtypes expressed in Xenopus oocytes. J Pharmacol Exp Ther 291:464-73 (1999). Read more (PubMed: 10525060) »
- Endo T et al. Comparative effects of nicardipine, a new calcium antagonist, on size of myocardial infarction after coronary artery occlusion in dogs. Circulation 74:420-30 (1986). Read more (PubMed: 3731430) »