Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8528] to Nicalin - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
製品名Anti-Nicalin antibody [EPR8528] - BSA and Azide free
Nicalin 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR8528] to Nicalin - BSA and Azide free
アプリケーション適用あり: WBmore details
適用なし: Flow Cyt,ICC,IHC-P or IP
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human Nicalin aa 50-150. The exact sequence is proprietary.
Database link: Q969V3
Ab248361 is the carrier-free version of ab131159. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab248361 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
解離定数（KD 値）KD = 8.10 x 10 -11 M Learn more about KD
Concentration information loading...
Our Abpromise guarantee covers the use of ab248361 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 63 kDa).|
機能May antagonize Nodal signaling and subsequent organization of axial structures during mesodermal patterning.
組織特異性Highly expressed in pancreas and skeletal muscle and, at lower levels, in heart.
配列類似性Belongs to the nicastrin family.
細胞内局在Endoplasmic reticulum membrane.
- Information by UniProt
- NCLN antibody
- NCLN_HUMAN antibody
- NET59 antibody
All lanes : Anti-Nicalin antibody [EPR8528] (ab131159) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : BxPC-3 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : C6 cell lysate
Lane 5 : Raw 264.7 cell lysate
Lane 6 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 63 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
This data was developed using ab131159, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.