Anti-NHE-7 抗体 (ab272649)
Key features and details
- Rabbit polyclonal to NHE-7
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-NHE-7 antibody
NHE-7 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NHE-7 -
由来種
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
-
免疫原
Recombinant fragment corresponding to Human NHE-7 aa 600-700.
Database link: Q96T83 -
ポジティブ･コントロール
- WB: RT4 and U-251 MG cells. IHC-P: Human kidney tissue.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272649 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| IHC-P |
Human
|
| WB |
Human
|
| All applications |
Mouse
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
| 特記事項 |
|---|
|
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ターゲット情報
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機能
Mediates electroneutral exchange of protons for Na(+) and K(+) across endomembranes. May contribute to Golgi volume and cation homeostasis. -
組織特異性
Ubiquitously expressed. -
配列類似性
Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family. -
細胞内局在
Golgi apparatus, trans-Golgi network membrane. Recycling endosome membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 84679 Human
- Entrez Gene: 236727 Mouse
- Omim: 300368 Human
- SwissProt: Q96T83 Human
- SwissProt: Q8BLV3 Mouse
- Unigene: 91389 Human
- Unigene: 132983 Mouse
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別名
- Na(+)/H(+) exchanger 7 antibody
- NHE-7 antibody
- NHE7 antibody
see all
画像
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Western blot - Anti-NHE-7 antibody (ab272649)Lanes 2-3 : Anti-NHE-7 antibody (ab272649) at 0.4 µg/ml
Lane 1 : Molecular Weight ladder
Lane 2 : RT4 cells (Human urinary bladder cancer cell line)
Lane 3 : U-251 MG cells (Human brain glioma cell line) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NHE-7 antibody (ab272649)Paraffin-embedded formalin-fixed human kidney tissue stained for NHE-7 using ab272649 at 1/20 dilution in immunohistochemical analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab272649 は論文での使用が確認できていません。
