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Anti-NGF 抗体 (ab6199)

  • Datasheet
  • SDS
Reviews (8)Q&A (20)References (52)

Product price, shipping and contact information

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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-NGF antibody (ab6199)
  • Western blot - Anti-NGF antibody (ab6199)
  • Immunocytochemistry/ Immunofluorescence - Anti-NGF antibody (ab6199)

Key features and details

  • Rabbit polyclonal to NGF
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat, Chicken, Human
  • Isotype: IgG

こちらの製品もご検討ください

一次抗体
Product image
Anti-TrkB antibody (ab18987)
二次抗体
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
タンパク質
Recombinant human NGF protein (Animal Free) (ab217472)

関連製品

製品の概要

  • 製品名

    Anti-NGF antibody
    NGF 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to NGF
  • 由来種

    Rabbit
  • 特異性

    Less than 1% cross-reactivity against recombinant human Brain Derived Neurotrophic Factor, Neurotrophin 3 and Neurotrophin 4/5 by ELISA.
  • アプリケーション

    適用あり: ICC/IF, WBmore details
  • 種交差性

    交差種: Mouse, Rat, Chicken, Human
    非交差種: Cow
  • 免疫原

    Full length native protein (purified) corresponding to Mouse NGF.
    Database link: P01139

  • ポジティブ・コントロール

    • ウェスタン・ブロット用ポジティブコントロールRecombinant human NGF protein (Animal Free)
  • 特記事項

    This antibody has been shown to be useful for a variety of techniques and its specificity has been demonstrated by immunoblot.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7
    Constituent: PBS

    pH: 7.2-7.6 without preservatives.
  • Concentration information loading...
  • 精製度

    Protein G purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Neuroscience
    • Neurology process
    • Growth and Development
    • Neurotrophins
    • Neuroscience
    • Neurology process
    • Neuroregeneration
    • Neuroregeneration

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human NGF protein (Animal Free) (ab217472)

アプリケーション

Our Abpromise guarantee covers the use of ab6199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能

    Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177).
  • 関連疾患

    Neuropathy, hereditary sensory and autonomic, 5
  • 配列類似性

    Belongs to the NGF-beta family.
  • 細胞内局在

    Secreted.
  • Target information above from: UniProt accession P01138 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 4803 Human
    • Entrez Gene: 18049 Mouse
    • Entrez Gene: 310738 Rat
    • Omim: 162030 Human
    • SwissProt: P05200 Chicken
    • SwissProt: P01138 Human
    • SwissProt: P01139 Mouse
    • SwissProt: P25427 Rat
    • Unigene: 2561 Human
    • Unigene: 1259 Mouse
    • Unigene: 22168 Rat
    see all
  • 別名

    • Beta nerve growth factor antibody
    • Beta NGF antibody
    • Beta-nerve growth factor antibody
    • Beta-NGF antibody
    • HSAN5 antibody
    • MGC161426 antibody
    • MGC161428 antibody
    • Nerve growth factor (beta polypeptide) antibody
    • Nerve growth factor antibody
    • Nerve growth factor beta antibody
    • Nerve growth factor beta polypeptide antibody
    • Nerve growth factor beta subunit antibody
    • NGF antibody
    • NGF_HUMAN antibody
    • NGFB antibody
    • NID67 antibody
    see all

画像

  • Western blot - Anti-NGF antibody (ab6199)
    Western blot - Anti-NGF antibody (ab6199)
    All lanes : Anti-NGF antibody (ab6199) at 5 µg/ml

    Lane 1 : rhNGF protein at 100 µg
    Lane 2 : Mouse salivary gland tissue lysate at 50 µg


    ab6199 detects a strong band at 13 kDa consistent with the expected molecular weight of mature NGF monomer.

  • Western blot - Anti-NGF antibody (ab6199)
    Western blot - Anti-NGF antibody (ab6199)
    Anti-NGF antibody (ab6199) at 1 µg/ml + Active human NGF full length protein (ab69759) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 30 seconds
  • Immunocytochemistry/ Immunofluorescence - Anti-NGF antibody (ab6199)
    Immunocytochemistry/ Immunofluorescence - Anti-NGF antibody (ab6199)
    ICC/IF image of ab6199 stained MEF1 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6199, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (52)

    ab6199 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab6199 は 52 報の論文で使用されています。

    • Zheng Y  et al. Altered expression of glycoprotein non-metastatic melanoma protein B in the distal sciatic nerve following injury. Int J Mol Med 45:1909-1917 (2020). PubMed: 32236569
    • Yuan H  et al. Hypomethylation of nerve growth factor (NGF) promotes binding of C/EBPa and contributes to inflammatory hyperalgesia in rats. J Neuroinflammation 17:34 (2020). PubMed: 31980031
    • Liao D  et al. Involvement of neurotrophic signaling in doxorubicin-induced cardiotoxicity. Exp Ther Med 19:1129-1135 (2020). PubMed: 32010279
    • Sun Q  et al. microRNA-9 and -29a regulate the progression of diabetic peripheral neuropathy via ISL1-mediated sonic hedgehog signaling pathway. Aging (Albany NY) 12:11446-11465 (2020). PubMed: 32544883
    • Jung DH  et al. Therapeutic effects of anodal transcranial direct current stimulation in a rat model of ADHD. Elife 9:N/A (2020). PubMed: 32955434
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 28 Abreviews or Q&A

    Western blot abreview for Anti-NGF antibody

    Excellent
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (gastric cancer cell)
    Gel Running Conditions
    Reduced Denaturing (15% gel)
    Loading amount
    30 µg
    Specification
    gastric cancer cell
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1µg/mL · Temperature: 24°C
    Read More

    Mr. JUNGHO YOON

    Verified customer

    投稿 Aug 16 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NGF antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Dako FLEX Peroxidase blocking as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako High pH
    Sample
    Human Tissue sections (Tendon)
    Specification
    Tendon
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 15 2014

    Question

    Can you suggest a positive control for Western blotting?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 11 2013

    Answer

    Several different lysates could be used as a positive control. We have used ab6199 with MEF1 cell line in Western blotting. Alternatively it has also been used with rat brain tissue and should also work with mouse or human brain tissue. We have a range of different brain lysates. These can be viewed under the following catalogue numbers:

    Brain (Human) Tissue Lysate - adult normal tissue (ab29466)

    Brain (Mouse) Whole Cell Lysate - normal tissue (ab30151)

    Brain (Rat) Whole Cell Lysate - normal tissue (ab29475)

    Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188)

    Read More

    Abcam Scientific Support

    Answered on Mar 11 2013

    Question


    Inquiry: For neutralization studies using this anti-NGF antibody, what control rabbit IgG product do you recommend?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 08 2012

    Answer

    Thank you for contacting Abcam.

    I beleive the rabbit IgG control below would work very well for your application.

    https://www.abcam.com/Rabbit-IgG-ChIP-Grade-ab37415.html



    Also, please see the attachment to find out details of our current promotion, of buy one antibody, get a RabMab for free.

    Read More

    Abcam Scientific Support

    Answered on Nov 08 2012

    Question

    Sehr geehrte Damen und Herren,
    gerade haben wir den AB NGF ( ab 6199) erhalten.
    In den tech notes wird geraten, glycerol hinzuzugeben.
    " add glyceerol to a final volume of 50 %".
    Wie soll dies denn verstanden werden. Unser Ab hat ein Volumen von 500 µl.
    Könnten sie die Logik dahinter kurz erläutern. Wir haben gerade darüber
    schwer gegrübelt
    Es gab verschiedene Ansätze und eine Wette dazu. ;-)
    Besten Dank für ihre Antwort
    Mit freundlichen Grüßen
    --

    Read More

    Abcam community

    Verified customer

    Asked on Nov 05 2012

    Answer

    Vielen Dank für Ihre Anfrage.
    Bitte geben Sie bei einem Volumen von 500ul Antikörper 500ul Glycerin (Glycerol) dazu.
    Das ergibt eine Endkonzentration von 50% Glycerin in 1000ul.
    Ich hoffe, der Wetteinsatz ist interessant :-)

    Read More

    Abcam Scientific Support

    Answered on Nov 05 2012

    Question

    Bonjour,
    Merci beaucoup pour votre retour. Je suis disponible toute la matinée au
    Cordialement,

    Read More

    Abcam community

    Verified customer

    Asked on Oct 24 2012

    Answer

    Suite à notre conversation téléphonique, je vous envoie la référence de la publication de Emoto où différentes méthodes de démasquage d'antigène ont été testées et comparées : Emoto et al., J Histochem Cytochem. 2005. 53 (11) : 1311-21.

    J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

    Read More

    Abcam Scientific Support

    Answered on Oct 24 2012

    Question

    Hi,
    I was looking to use the Anti-NGF antibody (ab6199)for Western blotting (reducing conditions)in mouse heart lysates. Thespecification sheet has a picture of a Western blot witha band corresponding to ˜25-37kDa.
    Just wondering if you knew a definitive MW that we should be expecting bands at?
    Doesthe antibodydetect NGF precursor and mature forms?
    Any additionalinformationon the running conditions for the blot shown in the specification sheetwould be greatly appreciated.

    Cheers,

    Read More

    Abcam community

    Verified customer

    Asked on Sep 14 2012

    Answer

    Thank you for your inquiry.

    The calculated molecular weight of NGF is 27kDa according to UniProt (http://www.uniprot.org/uniprot/P01138).

    This molecular weight will be higher when the protein is phosphorylated and glycosylated. According to the SwissProt page there are multiple modifications in NGF.

    We have customer feedback in form of an Abreview that will provide some more protocol information for you:

    https://www.abcam.com/NGF-antibody-ab6199.html&tab=abreviews&intabreviewid=29302

    There are also publications that might be helpful:

    Zhao LH et al. Cornel iridoid glycoside improves memory ability and promotes neuronal survival in fimbria-fornix transected rats. Eur J Pharmacol 647:68-74 (2010). WB; Rat. PubMed: 20826142
    Maezawa I et al. Rett syndrome astrocytes are abnormal and spread MeCP2 deficiency through gap junctions. J Neurosci 29:5051-61 (2009). WB; Mouse. PubMed: 19386901



    I hope this information is helpful and wish you good luck with your experiments.

    Read More

    Abcam Scientific Support

    Answered on Sep 14 2012

    Question


    I am a current user of theAnti-NGF (ab6199), I was wondering whether I could obtain some further information regarding this product.



    1. What is the optimal antigen retreival method.



    2.The pH of the antigen retreival solution, if any.



    3.The optimal dilutionof the Anti-NGF (ab6199).



    4. References on usage of the Anti-NGF (ab6199).

    Read More

    Abcam community

    Verified customer

    Asked on Jun 22 2012

    Answer

    Thank you for your enquiry.

    Here are answers to your questions:

    1.So far we know heat mediated antigen retrieval has worked.

    2. citrate buffer pH 6,0

    3. Optimal dilution must be determined emperically, but 5ug/ml has been used succesfully.

    4. Here is a link to published papers using ab6199:
    https://www.abcam.com/NGF-antibody-ab6199-references.html

    I hope this is helpful. Please contact us again if you have any further questions.

    Read More

    Abcam Scientific Support

    Answered on Jun 22 2012

    Question

    Product code: 6199
    Lot number: GR74133-1

    Inquiry: Hello I'm trying to make the '5 % acetic acid buffer containing 0.9 % NaCL (ASB) at pH 3' for perfusion prior to fixing as your protocol states. Could you let me know a recipie for this please? Does the acetic acid buffer contain NaAcetate as well? I have tried to look at the reference you provide (Zhou et al 1994) but can not access it. Kind regards

    Read More

    Abcam community

    Verified customer

    Asked on Jun 15 2012

    Answer

    Thank you for your inquiry about a reference that was mentioned by a customer that submitted an Abreview (http://www.ncbi.nlm.nih.gov/pubmed/7815824)


    Unfortunately, we do not have access to the specific article that was mentioned, nor do we have subscriptions to these journals. If you would like additional information regarding specific protocols or samples used, we would like to suggest contacting the authors of the article directly or ask your library to order the article for you.

    For advice regarding our products and protocols, we are happy to assist you. Please do not hesitate to contact us if you would like additional information.

    Read More

    Abcam Scientific Support

    Answered on Jun 15 2012

    Question

    Désolée j'ai oublié de vous préciser le tissus, c'est du cerveau humain.

    Read More

    Abcam community

    Verified customer

    Asked on Jun 13 2012

    Answer

    Merci pour votre réponse et ces précisions.

    J'ai de nouveau regardé vos marquages et je validerais personnellement ces résultats. Les deux anticorps montrent un bon marquage spécifique et lescontroles négatifs avec l'isotype sont d'aucune ambiguité.

    J'espère que ceci vous sera utile. Merci de nous avoir contactés. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

    Read More

    Abcam Scientific Support

    Answered on Jun 13 2012

    1-10 of 28 Abreviews or Q&A

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    For licensing inquiries, please contact partnerships@abcam.com

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