Anti-NGF 抗体 (ab6199)
Key features and details
- Rabbit polyclonal to NGF
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Chicken, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-NGF antibody
NGF 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NGF -
由来種
Rabbit -
特異性
Less than 1% cross-reactivity against recombinant human Brain Derived Neurotrophic Factor, Neurotrophin 3 and Neurotrophin 4/5 by ELISA. -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Rat, Chicken, Human
非交差種: Cow -
免疫原
Full length native protein (purified) corresponding to Mouse NGF.
Database link: P01139 -
ポジティブ･コントロール
-
特記事項
This antibody has been shown to be useful for a variety of techniques and its specificity has been demonstrated by immunoblot.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Constituent: PBS
pH: 7.2-7.6 without preservatives. -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab6199 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use a concentration of 10 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. |
ターゲット情報
-
機能
Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177). -
関連疾患
Neuropathy, hereditary sensory and autonomic, 5 -
配列類似性
Belongs to the NGF-beta family. -
細胞内局在
Secreted. - Information by UniProt
-
参照データベース
- Entrez Gene: 4803 Human
- Entrez Gene: 18049 Mouse
- Entrez Gene: 310738 Rat
- Omim: 162030 Human
- SwissProt: P05200 Chicken
- SwissProt: P01138 Human
- SwissProt: P01139 Mouse
- SwissProt: P25427 Rat
see all -
別名
- Beta nerve growth factor antibody
- Beta NGF antibody
- Beta-nerve growth factor antibody
see all
画像
-
Western blot - Anti-NGF antibody (ab6199)All lanes : Anti-NGF antibody (ab6199) at 5 µg/ml
Lane 1 : rhNGF protein at 100 µg
Lane 2 : Mouse salivary gland tissue lysate at 50 µgab6199 detects a strong band at 13 kDa consistent with the expected molecular weight of mature NGF monomer.
-
Western blot - Anti-NGF antibody (ab6199) -
Immunocytochemistry/ Immunofluorescence - Anti-NGF antibody (ab6199)ICC/IF image of ab6199 stained MEF1 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6199, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (52)
ab6199 は 52 報の論文で使用されています。
- Zheng Y et al. Altered expression of glycoprotein non-metastatic melanoma protein B in the distal sciatic nerve following injury. Int J Mol Med 45:1909-1917 (2020). PubMed: 32236569
- Yuan H et al. Hypomethylation of nerve growth factor (NGF) promotes binding of C/EBPa and contributes to inflammatory hyperalgesia in rats. J Neuroinflammation 17:34 (2020). PubMed: 31980031
- Liao D et al. Involvement of neurotrophic signaling in doxorubicin-induced cardiotoxicity. Exp Ther Med 19:1129-1135 (2020). PubMed: 32010279
- Sun Q et al. microRNA-9 and -29a regulate the progression of diabetic peripheral neuropathy via ISL1-mediated sonic hedgehog signaling pathway. Aging (Albany NY) 12:11446-11465 (2020). PubMed: 32544883
- Jung DH et al. Therapeutic effects of anodal transcranial direct current stimulation in a rat model of ADHD. Elife 9:N/A (2020). PubMed: 32955434
